Reviewed, UniProtKB/Swiss-Prot A6VI14 (DP2L_METM7)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	MmarC7_1023

Organism

Methanococcus maripaludis (strain C7 / ATCC BAA-1331) [Complete proteome] [HAMAP]

Taxonomic identifier

426368 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	1131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1131	1131	DNA polymerase II large subunit		PRO_1000019390

Sequences

	Sequence		Length	Mass (Da)
	A6VI14-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 143EE34A3D4BE0B5	FASTA	1,131	128,380
	10 20 30 40 50 60 MLHVSASKGM TEYFKNILDN VTELYTLADD CRKSGYDVTD HVEIPLAKDM ADRVEGIVGP 70 80 90 100 110 120 KNVAERIREL VSDLGKEPAA LEIAKEIVEG KFGEFGREVG AEQAVRTALA VITEGIVAAP 130 140 150 160 170 180 LEGIAHVKIK KNNDSGEYLA IYFAGPIRSA GGTAQALAVL VGDYVRKNMG LDKFKPTEDE 190 200 210 220 230 240 VERYGEEVDL YQSEVTTFQY QPKAEEIRAA VRNISVEITG EATDDVEVSG HRDLPRVETN 250 260 270 280 290 300 QIRGGALLAL VEGVLLKAPK ILRHVDKLGI EGWDWLKELK SKKEEVIEEI EEEKDDFNYE 310 320 330 340 350 360 EEEDLSQYED YEVEAVTKFI GEVIAGRPVF SHPSKKGGFR LRYGRSRNTG FATDGFHPAI 370 380 390 400 410 420 MYLVDDFMAV GTQLKTERPG KATCVVPVDS IEGPIVRLKD KSVLKIDTVE KAKQYRDDVE 430 440 450 460 470 480 EILFLGDILV NYGDFLENNH TILPSSWCTE WYEKILKSEN LEYKKEFIEN PDQKEAVNYA 490 500 510 520 530 540 KLTKTPLHPK YTYFWHDISK DNINVLRNWI IGGKYNESND SWEVTYDSEN PEISNAKRYL 550 560 570 580 590 600 ELIGCPHTVT GEKLEIFEYY PLLYSLGYDF DEKRDIVEEI EEKLQNTKNN MHFINTIAPF 610 620 630 640 650 660 EIRRNAYIYV GARMGRPEKA ASRKMKPPVN GLFPIGNAGA LVRLINKAVE EGKTDEIEIS 670 680 690 700 710 720 NVKCSCGNVS LYRTCPFCGN SVEPSGPSRI KLPIKEYWYK ALENLKINKA GDVKCIKGMT 730 740 750 760 770 780 SKDKIIEPLE KAILRAKNDI YVFKDGTTRF DCTDVPVTHF RPVEIHVGIE KLKSLGYLKD 790 800 810 820 830 840 IHGNSLENED QVLELKVQDV IVPESCMDYF FNVSKFIDDL LEKYYKKDRF YNVNKRDDLV 850 860 870 880 890 900 GHLIIGMAPH TSAGMVGRII GYSNANVGYA HPYFHASKRR NCDGDEDAFF LLLDAFMNFS 910 920 930 940 950 960 KRFLPDKRGG QMDAPLVLTT ILDPKEVDGE VHNMDSMWEY PIEFYEKSLE GIAPKEIKKI 970 980 990 1000 1010 1020 MQTVEDRLDK DSQYEGIGYT HETLKIDEGP LVCAYKTLGS MMEKTSAQLA VAKKIRATDE 1030 1040 1050 1060 1070 1080 RDVAEKVIQS HFVPDLIGNL RAFSRQGVRC KCGAKYRRMP LKGVCRKCGS RLILTVSKGA 1090 1100 1110 1120 1130 VEKYMDVSQT MAEKYEASDY IKQRLEIIRS GIDSLFVNDK RKQVKIEDFF K

References

[1]

"Complete sequence of Methanococcus maripaludis C7." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000745 Genomic DNA. Translation: ABR66090.1.
RefSeq	YP_001330241.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5329159.
GenomeReviews	Gene locus MmarC7_1023 in contig CP000745_GR.
KEGG	mmz:MmarC7_1023.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METM7

Accession

Primary (citable) accession number: A6VI14

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	July 22, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)