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A6VGH7

- BIOB_METM7

UniProt

A6VGH7 - BIOB_METM7

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Protein
Biotin synthase
Gene
bioB, MmarC7_0484
Organism
Methanococcus maripaludis (strain C7 / ATCC BAA-1331)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi142 – 1421Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi201 – 2011Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi277 – 2771Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMMAR426368:GHHL-486-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:MmarC7_0484
OrganismiMethanococcus maripaludis (strain C7 / ATCC BAA-1331)
Taxonomic identifieri426368 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000002298: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Biotin synthaseUniRule annotation
PRO_0000381465Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi426368.MmarC7_0484.

Structurei

3D structure databases

ProteinModelPortaliA6VGH7.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiRDFHGMA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A6VGH7-1 [UniParc]FASTAAdd to Basket

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MKEIELNSDS LEIYEKSASE KLNKKDLVDL WNLDLKNLLD ISYSLKKLFN    50
KEKIDLCSIM NAKSGVCSEN CIFCSQSKHN SSKIDTYELK SKEEILKNAK 100
SVEKYSNRFS IVVSGKSVTD LEFENIIESI EEIQNKTKLK VCVSLGLLNK 150
DKLKALKEKN VRIHNNLETS ENYFKNICTS HDYIDKIKVI LEAKKMGLEM 200
CSGGIFGMGE SVADRVDLLL DLKKLDVDSV ALNLLNPICG TRIYDKINSG 250
EFIEINHTDA LKSICIARIA LPKKVIRLCG GREHVLKDMQ KYSLYVLDGL 300
MIGNYLTTNG QNIQSDLKMI DEMGFKQ 327
Length:327
Mass (Da):37,071
Last modified:August 21, 2007 - v1
Checksum:i2BB43BA425ADAB06
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000745 Genomic DNA. Translation: ABR65553.1.
RefSeqiWP_011976885.1. NC_009637.1.
YP_001329704.1. NC_009637.1.

Genome annotation databases

EnsemblBacteriaiABR65553; ABR65553; MmarC7_0484.
GeneIDi5329470.
KEGGimmz:MmarC7_0484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000745 Genomic DNA. Translation: ABR65553.1 .
RefSeqi WP_011976885.1. NC_009637.1.
YP_001329704.1. NC_009637.1.

3D structure databases

ProteinModelPortali A6VGH7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 426368.MmarC7_0484.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR65553 ; ABR65553 ; MmarC7_0484 .
GeneIDi 5329470.
KEGGi mmz:MmarC7_0484.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi RDFHGMA.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MMAR426368:GHHL-486-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C7 / ATCC BAA-1331.

Entry informationi

Entry nameiBIOB_METM7
AccessioniPrimary (citable) accession number: A6VGH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: September 3, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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