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A6VGH7 (BIOB_METM7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:MmarC7_0484
OrganismMethanococcus maripaludis (strain C7 / ATCC BAA-1331) [Complete proteome] [HAMAP]
Taxonomic identifier426368 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 327327Biotin synthase HAMAP-Rule MF_01694
PRO_0000381465

Sites

Metal binding671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding741Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1421Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2011Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2771Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A6VGH7 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 2BB43BA425ADAB06

FASTA32737,071
        10         20         30         40         50         60 
MKEIELNSDS LEIYEKSASE KLNKKDLVDL WNLDLKNLLD ISYSLKKLFN KEKIDLCSIM 

        70         80         90        100        110        120 
NAKSGVCSEN CIFCSQSKHN SSKIDTYELK SKEEILKNAK SVEKYSNRFS IVVSGKSVTD 

       130        140        150        160        170        180 
LEFENIIESI EEIQNKTKLK VCVSLGLLNK DKLKALKEKN VRIHNNLETS ENYFKNICTS 

       190        200        210        220        230        240 
HDYIDKIKVI LEAKKMGLEM CSGGIFGMGE SVADRVDLLL DLKKLDVDSV ALNLLNPICG 

       250        260        270        280        290        300 
TRIYDKINSG EFIEINHTDA LKSICIARIA LPKKVIRLCG GREHVLKDMQ KYSLYVLDGL 

       310        320 
MIGNYLTTNG QNIQSDLKMI DEMGFKQ 

« Hide

References

[1]"Complete sequence of Methanococcus maripaludis C7."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sieprawska-Lupa M., Whitman W.B., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C7 / ATCC BAA-1331.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000745 Genomic DNA. Translation: ABR65553.1.
RefSeqYP_001329704.1. NC_009637.1.

3D structure databases

ProteinModelPortalA6VGH7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING426368.MmarC7_0484.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR65553; ABR65553; MmarC7_0484.
GeneID5329470.
KEGGmmz:MmarC7_0484.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.
ProtClustDBCLSK876516.

Enzyme and pathway databases

BioCycMMAR426368:GHHL-486-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_METM7
AccessionPrimary (citable) accession number: A6VGH7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: February 19, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways