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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Methanococcus maripaludis (strain C7 / ATCC BAA-1331)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11SubstrateUniRule annotation1
Binding sitei62SubstrateUniRule annotation1
Active sitei71Proton donorUniRule annotation1
Binding sitei160SubstrateUniRule annotation1
Sitei162Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei193SubstrateUniRule annotation1
Sitei211Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei220Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciMMAR426368:G1G9C-166-MONOMER
UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:MmarC7_0164
OrganismiMethanococcus maripaludis (strain C7 / ATCC BAA-1331)
Taxonomic identifieri426368 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000002298 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001244231 – 277Diaminopimelate epimeraseAdd BLAST277

Proteomic databases

PRIDEiA6VFK8

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi426368.MmarC7_0164

Structurei

3D structure databases

ProteinModelPortaliA6VFK8
SMRiA6VFK8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni72 – 73Substrate bindingUniRule annotation2
Regioni211 – 212Substrate bindingUniRule annotation2
Regioni221 – 222Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02255 Archaea
COG0253 LUCA
HOGENOMiHOG000220466
KOiK01778
OMAiSMCGNGG
OrthoDBiPOG093Z085A

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

A6VFK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTKMHGLG NDYIYVDATS QKIENPNEIS RFVSDRHFGI GSDGLVLILP
60 70 80 90 100
SEIADFKMRM FNSDGSEAEM CGNAIRCVGK FVYDKKMTDK STITIETLAG
110 120 130 140 150
IKVLEMTVEN DKVVLVKVDM GEPILKAETI PVLSEKHPVI DEEITAKDYC
160 170 180 190 200
YNFTCVSMGN PHAITYIENV SEFPLEKIGP LFEFHEKFPR KTNVEFVELI
210 220 230 240 250
DDSTIKMRVW ERGAGETLAC GTGACAVLVA SVLKGYVGRK ATVKLLGGDL
260 270
TIEWNESDNH IYMTGPATTV FEGEIDI
Length:277
Mass (Da):30,798
Last modified:August 21, 2007 - v1
Checksum:i5398BFB706F37D10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000745 Genomic DNA Translation: ABR65234.1
RefSeqiWP_011976571.1, NC_009637.1

Genome annotation databases

EnsemblBacteriaiABR65234; ABR65234; MmarC7_0164
GeneIDi5328025
KEGGimmz:MmarC7_0164

Similar proteinsi

Entry informationi

Entry nameiDAPF_METM7
AccessioniPrimary (citable) accession number: A6VFK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: May 23, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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