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Protein

NAD/NADP-dependent betaine aldehyde dehydrogenase

Gene

betB

Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid.UniRule annotation

Catalytic activityi

Betaine aldehyde + NAD+ + H2O = betaine + NADH.UniRule annotation

Cofactori

K(+)UniRule annotationNote: Binds 2 potassium ions per subunit.UniRule annotation

Pathway:ibetaine biosynthesis via choline pathway

This protein is involved in step 1 of the subpathway that synthesizes betaine from betaine aldehyde.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NAD/NADP-dependent betaine aldehyde dehydrogenase (betB)
This subpathway is part of the pathway betaine biosynthesis via choline pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from betaine aldehyde, the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi26 – 261Potassium 1UniRule annotation
Metal bindingi27 – 271Potassium 1; via carbonyl oxygenUniRule annotation
Metal bindingi93 – 931Potassium 1UniRule annotation
Active sitei162 – 1621Charge relay systemUniRule annotation
Metal bindingi180 – 1801Potassium 1; via carbonyl oxygenUniRule annotation
Binding sitei209 – 2091NAD/NADP; via amide nitrogenUniRule annotation
Metal bindingi246 – 2461Potassium 2; via carbonyl oxygenUniRule annotation
Sitei248 – 2481Seems to be a necessary countercharge to the potassium cationsUniRule annotation
Active sitei252 – 2521Proton acceptorUniRule annotation
Binding sitei286 – 2861NAD/NADPUniRule annotation
Binding sitei387 – 3871NAD/NADPUniRule annotation
Metal bindingi457 – 4571Potassium 2; via carbonyl oxygenUniRule annotation
Metal bindingi460 – 4601Potassium 2; via carbonyl oxygenUniRule annotation
Active sitei464 – 4641Charge relay systemUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi150 – 1534NAD/NADPUniRule annotation
Nucleotide bindingi176 – 1794NAD/NADPUniRule annotation
Nucleotide bindingi229 – 2346NAD/NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, NADP, Potassium

Enzyme and pathway databases

BioCyciPAER381754:GHMY-6156-MONOMER.
UniPathwayiUPA00529; UER00386.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD/NADP-dependent betaine aldehyde dehydrogenaseUniRule annotation (EC:1.2.1.8UniRule annotation)
Short name:
BADHUniRule annotation
Gene namesi
Name:betBUniRule annotation
Ordered Locus Names:PSPA7_6158
OrganismiPseudomonas aeruginosa (strain PA7)
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000001582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490NAD/NADP-dependent betaine aldehyde dehydrogenasePRO_1000047045Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei286 – 2861Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Dimer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6VEI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
KOiK00130.
OMAiKDGYYMR.
OrthoDBiEOG6BS8QW.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6VEI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARFEEQKLY IGGRYVEASS GATFETINPA NGEVLAKVQR ASREDVERAV
60 70 80 90 100
QSAVEGQKVW AAMTAMQRSR ILRRAVDLLR ERNDELAALE TLDTGKPLAE
110 120 130 140 150
TRAVDIVTGA DVLEYYAGLV PAIEGEQIPL RETSFVYTRR EPLGVVAGIG
160 170 180 190 200
AWNYPVQIAL WKSAPALAAG NAMIFKPSEV TPLTALKLAE IYTEAGVPDG
210 220 230 240 250
VFNVLTGSGR EVGQWLTEHP LIEKISFTGG TSTGKKVMAS ASSSSLKEVT
260 270 280 290 300
MELGGKSPLI IFPDADLDRA ADIAVMANFF SSGQVCTNGT RVFIHRSQQA
310 320 330 340 350
RFEAKVLERV QRIRLGDPQD ENTNFGPLVS FPHMESVLGY IESGKAQKAR
360 370 380 390 400
LLCGGERVTE GAFGNGAYVA PTVFTDCSDD MTIVREEIFG PVMSILVYDD
410 420 430 440 450
EDEAIRRAND TEYGLAAGVV TQDLARAHRA IHRLEAGICW INTWGESPAE
460 470 480 490
MPVGGYKQSG VGRENGLTTL AHYTRIKSVQ VELGDYASVF
Length:490
Mass (Da):53,247
Last modified:August 21, 2007 - v1
Checksum:i2BD4B051E2E2A4D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR80835.1.
RefSeqiWP_012077969.1. NC_009656.1.

Genome annotation databases

EnsemblBacteriaiABR80835; ABR80835; PSPA7_6158.
KEGGipap:PSPA7_6158.
PATRICi19833781. VBIPseAer80442_5858.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR80835.1.
RefSeqiWP_012077969.1. NC_009656.1.

3D structure databases

ProteinModelPortaliA6VEI4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR80835; ABR80835; PSPA7_6158.
KEGGipap:PSPA7_6158.
PATRICi19833781. VBIPseAer80442_5858.

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
KOiK00130.
OMAiKDGYYMR.
OrthoDBiEOG6BS8QW.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00386.
BioCyciPAER381754:GHMY-6156-MONOMER.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Dodson R.J., Harkins D., Paulsen I.T.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PA7.

Entry informationi

Entry nameiBETB_PSEA7
AccessioniPrimary (citable) accession number: A6VEI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: July 22, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.