Reviewed, UniProtKB/Swiss-Prot A6VEI3 (BETA_PSEA7)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	PSPA7_6157

Organism

Pseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]

Taxonomic identifier

381754 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Choline dehydrogenase		PRO_1000046566
Regions
	Nucleotide binding	6 – 35	30	FAD Probable
Sites
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VEI3-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 5E9E63FD6F955C5D	FASTA	561	61,892
	10 20 30 40 50 60 MSQEFDYIII GAGSAGNVLA TRLTEDADVS VLLLEAGGPD YRFDFRTQMP AALAFPLQGR 70 80 90 100 110 120 RYNWAYETDP EPYMNNRRME CGRGKGLGGS SLINGMCYIR GNALDFDGWA KEPGLEDWSY 130 140 150 160 170 180 LDCLPYFRKA ETRDIGPNDY HGGDGPVSVT TPKAGNNPLF HAMVEAGVQA GYPRTEDLNG 190 200 210 220 230 240 YQQEGFGPMD RTVTPEGRRA ATGRGYLDQA RGRPNLTIVT HALSDRILFS GKRAIGVSYL 250 260 270 280 290 300 VGNGDNPVTA HARREVLVCS GAIASPQLLQ RSGVGPAALL RDLDIPVVHD LPGVGANLQD 310 320 330 340 350 360 HLELYLQYAC KQPVSIYPAT KWWNQPAIGA QWLFLGKGLG ASNQFEAGGF IRTREAFEWP 370 380 390 400 410 420 NIQFHFLPVA INYNGSKGVQ EHGFQAHMGS MRSPSRGRIH LKSRDPRQHP SILFNYMSHE 430 440 450 460 470 480 QDWQEFRDGI RLTREIMNQP ALDPYRGREL SPGVNVQSDA ELDEFIRNHA ETAFHPSCSC 490 500 510 520 530 540 KMGSDDMAVV DGQGRVHGME GLRVVDASIM PLIITGNLNA TTIMMAEKIA DRIRGRQPLP 550 560 RSTAKYYVAG DAPVRGKPVR A

References

[1]	Dodson R.J., Harkins D., Paulsen I.T. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000744 Genomic DNA. Translation: ABR85536.1.
RefSeq	YP_001351468.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5357019.
GenomeReviews	Gene locus PSPA7_6157 in contig CP000744_GR.
KEGG	pap:PSPA7_6157.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_PSEA7

Accession

Primary (citable) accession number: A6VEI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	November 25, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)