Reviewed, UniProtKB/Swiss-Prot A6VCM8 (LLDD_PSEA7)

Names and originHide

Protein names

Recommended name:
L-lactate dehydrogenase [cytochrome]
EC=1.1.2.3

Gene names

Name:	lldD
Ordered Locus Names:	PSPA7_5491

Organism

Pseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]

Taxonomic identifier

381754 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributesHide

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+. HAMAP MF_01559
Cofactor	FMN By similarity. HAMAP MF_01559
Subunit structure	Homotetramer By similarity. HAMAP MF_01559
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

OntologiesHide

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	L-lactate dehydrogenase [cytochrome] HAMAP MF_01559		PRO_1000068988
Regions
	Domain	1 – 380	380	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

SequencesHide

	Sequence		Length	Mass (Da)
	A6VCM8-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: AB7EB6CC1AA39CE9	FASTA	381	41,123
	10 20 30 40 50 60 MIISASTDYR AAAQRKLPPF LFHYIDGGAY AEYTLRRNVE DLSAIALRQR VLKNMSELSL 70 80 90 100 110 120 ETRLFDETLA MPVALAPVGL TGMYARRGEV QAARAAAAKG VPFTLSTVSV CPIEEVAPAI 130 140 150 160 170 180 DRPMWFQLYV LKDRGFMRNA LERAKAAGVT TLVFTVDMPV PGARYRDAHS GMSGPYAAPR 190 200 210 220 230 240 RILQAMTHPA WAWDVGLLGK PHDLGNISTY RGNPTGLEDY IGWLGANFDP SISWKDLEWI 250 260 270 280 290 300 REFWNGPMVI KGILDPEDAK DAVKFGADGI VVSNHGGRQL DGVLSSARAL PAIADAVKGE 310 320 330 340 350 360 LAILADSGIR TGLDVVRMIA LGADSVLLGR AFVYALAAAG EAGVRNLLEL IEKEMRVAMV 370 380 LTGAKSIGEI SADSLVRELG A

ReferencesHide

[1]	Dodson R.J., Harkins D., Paulsen I.T. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	CP000744 Genomic DNA. Translation: ABR83637.1.
RefSeq	YP_001350813.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VCM8.
Genome annotation databases
GeneID	5354272.
GenomeReviews	Gene locus PSPA7_5491 in contig CP000744_GR.
KEGG	pap:PSPA7_5491.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1304.
HOGENOM	HBG517781.
OMA	WIREFWE.
ProtClustDB	PRK11197.
Family and domain databases
HAMAP	MF_01559. L-lact_dehydr. [Tree]
InterPro	IPR012133. a-Hydoxy_acid_DH_FMN. IPR013785. Aldolase_TIM. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. IPR020920. L-lactate_DHase_cyt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry informationHide

Entry name

LLDD_PSEA7

Accession

Primary (citable) accession number: A6VCM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	July 13, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)