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A6VBU7 (SYI_PSEA7) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:PSPA7_5200
OrganismPseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]
Taxonomic identifier381754 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length943 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 943943Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_1000022103

Regions

Motif58 – 6811"HIGH" region HAMAP-Rule MF_02002
Motif608 – 6125"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding9061Zinc By similarity
Metal binding9091Zinc By similarity
Metal binding9261Zinc By similarity
Metal binding9291Zinc By similarity
Binding site5671Aminoacyl-adenylate By similarity
Binding site6111ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6VBU7 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: F980FC538586E243

FASTA943105,233
        10         20         30         40         50         60 
MTDYKATLNL PETAFPMKAG LPQREPETLK FWNDIGLYQK LRAIGGDRPK FVLHDGPPYA 

        70         80         90        100        110        120 
NGSIHIGHAV NKILKDIIVR SKTLAGYDAP YVPGWDCHGL PIEHKVETTH GKNLPADKTR 

       130        140        150        160        170        180 
ELCREYAAEQ IEGQKADFIR LGVLGEWDNP YKTMAFANEA NEIRALAEMV RQDFVFKGLK 

       190        200        210        220        230        240 
PVNWCFDCGS ALAEAEVEYA DKTSPTIDVG FPVADADKLA AAFGLAALDK PAQIVIWTTT 

       250        260        270        280        290        300 
PWTIPANQAL NVHPEIDYAL VDAGDRYLVL AEALVEPCLA RYQREGKVVA TAKGEALELI 

       310        320        330        340        350        360 
NFRHPFYERL SPVYLADYVA LDAGTGIVHS SPAYGEDDFY TCKRYGMSND DILSPVQSNG 

       370        380        390        400        410        420 
VYVDSLPFFG GQFIWKANPN VVAKLEEVGS LLAHETINHS YMHCWRHKTP LIYRATAQWF 

       430        440        450        460        470        480 
VGMDKQPGQG ASLRERALEA ITQTEFIPGW GQARLHGMIA GRPDWCISRQ RNWGVPIPFF 

       490        500        510        520        530        540 
LHKASGELHP RTVELMEEVA QRVEKEGIEA WFRLDAAELL GDEAAQYDKI SDTLDVWFDS 

       550        560        570        580        590        600 
GTTHWHVLRG SHRIGHASGP VADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRQLLTHGF 

       610        620        630        640        650        660 
TVDESGRKMS KSLGNTVVPQ TVIDTLGADI LRLWVASTDY SGEIAVSQQI LQRSADAYRR 

       670        680        690        700        710        720 
IRNTTRFLLS NLNGFDPAKD LLPPEEMLAL DRWAVDRALL LQREIEEAYR EYRFWNVYSK 

       730        740        750        760        770        780 
VHNFCVQELG GFYLDIIKDR QYTTGANSVA RRSCQTALFH IAEALVRWIA PILAFTAEEV 

       790        800        810        820        830        840 
WKFLPGERVE SVMLATWYDG LGELPADAAL NREYWEQVMA VKAAVNKELE NQRAAKAVGG 

       850        860        870        880        890        900 
NLQAEVTLYA EDALQASLAR LGNELRFVLI TSTATLAPLA SAPADAVDSE VAGLKLKVVK 

       910        920        930        940 
SAHAKCGRCW HHREDVGQHA AHPELCGRCI ENIEGSGEVR HYA

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References

[1]Dodson R.J., Harkins D., Paulsen I.T.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PA7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000744 Genomic DNA. Translation: ABR81436.1.
RefSeqYP_001350532.1. NC_009656.1.

3D structure databases

ProteinModelPortalA6VBU7.
ModBaseSearch...

Protein-protein interaction databases

STRING381754.PSPA7_5200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR81436; ABR81436; PSPA7_5200.
GeneID5356928.
KEGGpap:PSPA7_5200.
PATRIC19831941. VBIPseAer80442_4959.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAKQVLTHG.
ProtClustDBPRK05743.

Enzyme and pathway databases

BioCycPAER381754:GHMY-5218-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_PSEA7
AccessionPrimary (citable) accession number: A6VBU7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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