Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei567Aminoacyl-adenylateUniRule annotation1
Binding sitei611ATPUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1
Metal bindingi926ZincUniRule annotation1
Metal bindingi929ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PSPA7_5200
OrganismiPseudomonas aeruginosa (strain PA7)
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000001582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221031 – 943Isoleucine--tRNA ligaseAdd BLAST943

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6VBU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi608 – 612"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiPIPFFLH.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6VBU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYKATLNL PETAFPMKAG LPQREPETLK FWNDIGLYQK LRAIGGDRPK
60 70 80 90 100
FVLHDGPPYA NGSIHIGHAV NKILKDIIVR SKTLAGYDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVETTH GKNLPADKTR ELCREYAAEQ IEGQKADFIR LGVLGEWDNP
160 170 180 190 200
YKTMAFANEA NEIRALAEMV RQDFVFKGLK PVNWCFDCGS ALAEAEVEYA
210 220 230 240 250
DKTSPTIDVG FPVADADKLA AAFGLAALDK PAQIVIWTTT PWTIPANQAL
260 270 280 290 300
NVHPEIDYAL VDAGDRYLVL AEALVEPCLA RYQREGKVVA TAKGEALELI
310 320 330 340 350
NFRHPFYERL SPVYLADYVA LDAGTGIVHS SPAYGEDDFY TCKRYGMSND
360 370 380 390 400
DILSPVQSNG VYVDSLPFFG GQFIWKANPN VVAKLEEVGS LLAHETINHS
410 420 430 440 450
YMHCWRHKTP LIYRATAQWF VGMDKQPGQG ASLRERALEA ITQTEFIPGW
460 470 480 490 500
GQARLHGMIA GRPDWCISRQ RNWGVPIPFF LHKASGELHP RTVELMEEVA
510 520 530 540 550
QRVEKEGIEA WFRLDAAELL GDEAAQYDKI SDTLDVWFDS GTTHWHVLRG
560 570 580 590 600
SHRIGHASGP VADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRQLLTHGF
610 620 630 640 650
TVDESGRKMS KSLGNTVVPQ TVIDTLGADI LRLWVASTDY SGEIAVSQQI
660 670 680 690 700
LQRSADAYRR IRNTTRFLLS NLNGFDPAKD LLPPEEMLAL DRWAVDRALL
710 720 730 740 750
LQREIEEAYR EYRFWNVYSK VHNFCVQELG GFYLDIIKDR QYTTGANSVA
760 770 780 790 800
RRSCQTALFH IAEALVRWIA PILAFTAEEV WKFLPGERVE SVMLATWYDG
810 820 830 840 850
LGELPADAAL NREYWEQVMA VKAAVNKELE NQRAAKAVGG NLQAEVTLYA
860 870 880 890 900
EDALQASLAR LGNELRFVLI TSTATLAPLA SAPADAVDSE VAGLKLKVVK
910 920 930 940
SAHAKCGRCW HHREDVGQHA AHPELCGRCI ENIEGSGEVR HYA
Length:943
Mass (Da):105,233
Last modified:August 21, 2007 - v1
Checksum:iF980FC538586E243
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR81436.1.
RefSeqiWP_012077314.1. NC_009656.1.

Genome annotation databases

EnsemblBacteriaiABR81436; ABR81436; PSPA7_5200.
KEGGipap:PSPA7_5200.
PATRICi19831941. VBIPseAer80442_4959.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR81436.1.
RefSeqiWP_012077314.1. NC_009656.1.

3D structure databases

ProteinModelPortaliA6VBU7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR81436; ABR81436; PSPA7_5200.
KEGGipap:PSPA7_5200.
PATRICi19831941. VBIPseAer80442_4959.

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiPIPFFLH.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_PSEA7
AccessioniPrimary (citable) accession number: A6VBU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.