A6VAU7 (DNAE2_PSEA7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	PSPA7_4841

Organism

Pseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]

Taxonomic identifier

381754 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	1024 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1024	1024	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000070592

Sequences

	Sequence		Length	Mass (Da)
	A6VAU7 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 8801214E9DE8C2F5	FASTA	1,024	114,629
	10 20 30 40 50 60 MSAYAELHCL SNFSFQRGAS SAAELFARAA RLGYRALAIT DECSLAGIVR AWQAAREYRV 70 80 90 100 110 120 KLLVGSEIRL EQGPKLVLLA EDLAGYQQLC RLITRGRRRA DKGSYRLLRE DLQPALDGVL 130 140 150 160 170 180 AIWLAEPGDD DDAAWLGECF PQRLWLGVEL HRGADDDARL RGLLALAARA QLPAVACGDV 190 200 210 220 230 240 HMHCRGRRAL QDCMTAIRNH LPVSEAGAHL FANGERHLRP LAALQGIYPE ALLAETLRIA 250 260 270 280 290 300 ERCRFDLEQL KYQYPRELVP AGHDPASWLR RLTEKGIRRR WPDGASAKVR EQIEKELKLI 310 320 330 340 350 360 SELGYESYFL TVQDIVGFAR RQKILCQGRG SAANSAVCFA LGITELDPER INLLFERFLS 370 380 390 400 410 420 RERNEPPDID VDFEHERREE VIQYVFNRYG RQRAALTAVV STYHGAGAVR DVAKALGLPP 430 440 450 460 470 480 DQVDALANCC GRWSDRAPSA ERLMEAGFDP QSAVLRRVLA LTGELVGFPR HLSQHPGGFV 490 500 510 520 530 540 ISEQPLDTLV PVENASMVER TVIQWDKDDL DAVGLLKVDV LALGMLSALR RSFDLLHELR 550 560 570 580 590 600 GGERLSLASI PSEDPATYAM ISRADTIGVF QIESRAQMAM LPRLRPEKFY DLVIQVAIVR 610 620 630 640 650 660 PGPIQGDMVH PYLRRRNGEE PVAYPSVELE KVFERTLGVP LFQEQVMELA IVAADYSPGE 670 680 690 700 710 720 ADELRRSMAA WKRHGGLEHH RERLTRGMLA NGYEADFAAR IFEQIKGFGS YGFPESHAAS 730 740 750 760 770 780 FALLTYASSW LKRHEPAAFA CALINSWPMG FYSPDQLLQD ARRHAIQTRP VDVRHSRWDC 790 800 810 820 830 840 SLEPIGQEQP AIRLGLRMIR GFREEDARRI EQARQAQSFT DVHDLGRRAG LDTRALELLA 850 860 870 880 890 900 DAGALRGLAG HRHKARWAIA GVEPQLPLFA EGPRIEEPAI SLPLPSRGEE LLSDYALLGT 910 920 930 940 950 960 TLGPHPLKLL RVQLKARRCR ASRELVGMEH GRPVRIAGLV VGRQRPQTAS GVTFITLEDE 970 980 990 1000 1010 1020 FGMVNVVVWH DLAERQRRPF LESRLLQVEG TLESSSGVRH VIAGRLQDLT PLLTGLDVRS RDFH

References

[1]	Dodson R.J., Harkins D., Paulsen I.T. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PA7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000744 Genomic DNA. Translation: ABR84074.1.
RefSeq	YP_001350182.1. NC_009656.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VAU7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5358357.
GenomeReviews	Gene locus PSPA7_4841 in contig CP000744_GR.
KEGG	pap:PSPA7_4841.
PATRIC	19831246. VBIPseAer80442_4621.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	EQPAIRL.
PhylomeDB	A6VAU7.
ProtClustDB	PRK05672.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_PSEA7

Accession

Primary (citable) accession number: A6VAU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program