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Protein

Acetate kinase

Gene

ackA

Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (PSPA7_4685)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumUniRule annotation1
Binding sitei17ATPUniRule annotation1
Binding sitei87SubstrateUniRule annotation1
Active sitei144Proton donor/acceptorUniRule annotation1
Sitei176Transition state stabilizerUniRule annotation1
Sitei237Transition state stabilizerUniRule annotation1
Metal bindingi381MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 208ATPUniRule annotation5
Nucleotide bindingi279 – 281ATPUniRule annotation3
Nucleotide bindingi327 – 331ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:PSPA7_4684
OrganismiPseudomonas aeruginosa (strain PA7)
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000001582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000022461 – 394Acetate kinaseAdd BLAST394

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6VAE3.
SMRiA6VAE3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000288399.
KOiK00925.
OMAiFMMQKEN.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiView protein in InterPro
IPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiView protein in Pfam
PF00871. Acetate_kinase. 1 hit.
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiView protein in PROSITE
PS01075. ACETATE_KINASE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

A6VAE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSRNILVIN CGSSSIKFAL VNEAHSLFPL HGLAERLGSR DAVLRWKRGG
60 70 80 90 100
DSDSLMIPNA DHRAALAQLL PMVQNAAGGK LHGIGHRVVH GGELFTHATR
110 120 130 140 150
LDGRVIEAIR ATAPLAPLHN PANLQGIEAA MTLFPGLPHV AVFDTAFHQS
160 170 180 190 200
LPEHAYRYAL PEALYREHGV RRYGFHGTSH RYVSHRAAEM AGLAVGDSSW
210 220 230 240 250
LSAHLGNGSS TCAIVNGQSL DTSMGLTPLE GLVMGTRSGD VDPNLHSHLA
260 270 280 290 300
RTLGWSLERI DSMLNNESGL LGLSNLSNDM RTLEQEREQG HPGAALAIEV
310 320 330 340 350
FCYRLAKSLA AMSCALPQLD GVIFTGGIGE NSPLVRAKTV AHLRLFDLRL
360 370 380 390
DQEANARCVR GVAGPIQATG HPRVLVIPTN EERQIALDTL ALLD
Length:394
Mass (Da):42,363
Last modified:August 21, 2007 - v1
Checksum:i2AB2FFBB0C1526A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR83754.1.
RefSeqiWP_004367272.1. NC_009656.1.

Genome annotation databases

EnsemblBacteriaiABR83754; ABR83754; PSPA7_4684.
KEGGipap:PSPA7_4684.

Similar proteinsi

Entry informationi

Entry nameiACKA_PSEA7
AccessioniPrimary (citable) accession number: A6VAE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: June 7, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families