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A6V924

- NAPA_PSEA7

UniProt

A6V924 - NAPA_PSEA7

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP
    6. oxidoreductase activity, acting on NAD(P)H Source: RefGenome

    GO - Biological processi

    1. cellular respiration Source: RefGenome
    2. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    3. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciPAER381754:GHMY-4205-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:PSPA7_4205
    OrganismiPseudomonas aeruginosa (strain PA7)
    Taxonomic identifieri381754 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000001582: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
    BLAST
    Chaini30 – 834805Periplasmic nitrate reductasePRO_1000069722Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi381754.PSPA7_4205.

    Structurei

    3D structure databases

    ProteinModelPortaliA6V924.
    SMRiA6V924. Positions 43-829.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiQMVYNLH.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A6V924-1 [UniParc]FASTAAdd to Basket

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    MNLTRREFAK ANAAAIAAAA AGLPILVRAS NLVTEADVTS LDWNKAPCRF    50
    CGTGCSVMVA TRDGQVVATH GDIKAEVNRG INCVKGYFLS KIMYGSDRLT 100
    RPLLRMKDGK FDKQGEFQPI SWEQAFDIMA EKFKAALKAK GPESVGMFGS 150
    GQWTVWEGYA ANKLFKAGLR SNNIDPNARH CMASAVMGFM RSFGMDEPMG 200
    CYDDIEATDS FVLWGSNMAE MHPVLWSRVT DRRLSAPQVK VAVLSTFEHR 250
    SFELADIPMV FKPQTDLIIL NYIANHIIES GAVNRDFVER HVRFAHGAED 300
    IGYGLRPDDP LEKKAKNADK ANTWSDIDFN AFAEFVKPYT LERAARESGV 350
    PAERLKALAE LYAAPRRKVV SFWTMGFNQH TRGVWANNLI YNIHLLTGKI 400
    SEPGNSPFSL TGQPSACGTA REVGTFSHRL PADLVVTNPK HRETAEKIWK 450
    VPAGTIQEKV GFHAVQQSRM LKDGVLNVYW TQVSNNMQAG PNVMQEVLPG 500
    WRNPDNFVIV SDVYPTVSAQ AADLILPSAM WVEKEGAFGN AERRTQFWHQ 550
    LVKAPGEAKS DLWQLVEFSK RFTTDEVWPA ELLAKAPELK GKTLYEVLFR 600
    NGQVDRFPAS DLAKGYANDE VDAFGFYIQK GLFEEYAAFG RGHGHDLAPF 650
    DAYHEARGLR WPVVDGKETR WRYREGYDPY VSKGSGVQFY GYPDKKAIVF 700
    ALPYEPPAEA PDRDYPFWLA TGRVLEHWHT GSMTARVPEL YKAVPDAVVY 750
    MHPDDARQLK LRRGSEVRVV SRRGEIRARV ETRGRNKPPQ GLVFVPFFDA 800
    NKLINKVTLD ATDPISKQTD YKKCAVRIEL LNLA 834
    Length:834
    Mass (Da):93,510
    Last modified:August 21, 2007 - v1
    Checksum:i0CAA2E0F04CE8707
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000744 Genomic DNA. Translation: ABR83935.1.
    RefSeqiWP_012076670.1. NC_009656.1.
    YP_001349559.1. NC_009656.1.

    Genome annotation databases

    EnsemblBacteriaiABR83935; ABR83935; PSPA7_4205.
    GeneIDi5355582.
    KEGGipap:PSPA7_4205.
    PATRICi19830036. VBIPseAer80442_4025.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000744 Genomic DNA. Translation: ABR83935.1 .
    RefSeqi WP_012076670.1. NC_009656.1.
    YP_001349559.1. NC_009656.1.

    3D structure databases

    ProteinModelPortali A6V924.
    SMRi A6V924. Positions 43-829.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 381754.PSPA7_4205.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR83935 ; ABR83935 ; PSPA7_4205 .
    GeneIDi 5355582.
    KEGGi pap:PSPA7_4205.
    PATRICi 19830036. VBIPseAer80442_4025.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi QMVYNLH.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci PAER381754:GHMY-4205-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Dodson R.J., Harkins D., Paulsen I.T.
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PA7.

    Entry informationi

    Entry nameiNAPA_PSEA7
    AccessioniPrimary (citable) accession number: A6V924
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3