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A6V924

- NAPA_PSEA7

UniProt

A6V924 - NAPA_PSEA7

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Protein
Periplasmic nitrate reductase
Gene
napA, PSPA7_4205
Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi55 – 551Iron-sulfur (4Fe-4S) By similarity
Metal bindingi83 – 831Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP
  6. oxidoreductase activity, acting on NAD(P)H Source: RefGenome

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. cellular respiration Source: RefGenome
  3. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciPAER381754:GHMY-4205-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:PSPA7_4205
OrganismiPseudomonas aeruginosa (strain PA7)
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000001582: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signal Reviewed prediction
Add
BLAST
Chaini30 – 834805Periplasmic nitrate reductaseUniRule annotation
PRO_1000069722Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi381754.PSPA7_4205.

Structurei

3D structure databases

ProteinModelPortaliA6V924.
SMRiA6V924. Positions 43-829.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiQMVYNLH.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6V924-1 [UniParc]FASTAAdd to Basket

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MNLTRREFAK ANAAAIAAAA AGLPILVRAS NLVTEADVTS LDWNKAPCRF    50
CGTGCSVMVA TRDGQVVATH GDIKAEVNRG INCVKGYFLS KIMYGSDRLT 100
RPLLRMKDGK FDKQGEFQPI SWEQAFDIMA EKFKAALKAK GPESVGMFGS 150
GQWTVWEGYA ANKLFKAGLR SNNIDPNARH CMASAVMGFM RSFGMDEPMG 200
CYDDIEATDS FVLWGSNMAE MHPVLWSRVT DRRLSAPQVK VAVLSTFEHR 250
SFELADIPMV FKPQTDLIIL NYIANHIIES GAVNRDFVER HVRFAHGAED 300
IGYGLRPDDP LEKKAKNADK ANTWSDIDFN AFAEFVKPYT LERAARESGV 350
PAERLKALAE LYAAPRRKVV SFWTMGFNQH TRGVWANNLI YNIHLLTGKI 400
SEPGNSPFSL TGQPSACGTA REVGTFSHRL PADLVVTNPK HRETAEKIWK 450
VPAGTIQEKV GFHAVQQSRM LKDGVLNVYW TQVSNNMQAG PNVMQEVLPG 500
WRNPDNFVIV SDVYPTVSAQ AADLILPSAM WVEKEGAFGN AERRTQFWHQ 550
LVKAPGEAKS DLWQLVEFSK RFTTDEVWPA ELLAKAPELK GKTLYEVLFR 600
NGQVDRFPAS DLAKGYANDE VDAFGFYIQK GLFEEYAAFG RGHGHDLAPF 650
DAYHEARGLR WPVVDGKETR WRYREGYDPY VSKGSGVQFY GYPDKKAIVF 700
ALPYEPPAEA PDRDYPFWLA TGRVLEHWHT GSMTARVPEL YKAVPDAVVY 750
MHPDDARQLK LRRGSEVRVV SRRGEIRARV ETRGRNKPPQ GLVFVPFFDA 800
NKLINKVTLD ATDPISKQTD YKKCAVRIEL LNLA 834
Length:834
Mass (Da):93,510
Last modified:August 21, 2007 - v1
Checksum:i0CAA2E0F04CE8707
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000744 Genomic DNA. Translation: ABR83935.1.
RefSeqiWP_012076670.1. NC_009656.1.
YP_001349559.1. NC_009656.1.

Genome annotation databases

EnsemblBacteriaiABR83935; ABR83935; PSPA7_4205.
GeneIDi5355582.
KEGGipap:PSPA7_4205.
PATRICi19830036. VBIPseAer80442_4025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000744 Genomic DNA. Translation: ABR83935.1 .
RefSeqi WP_012076670.1. NC_009656.1.
YP_001349559.1. NC_009656.1.

3D structure databases

ProteinModelPortali A6V924.
SMRi A6V924. Positions 43-829.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 381754.PSPA7_4205.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR83935 ; ABR83935 ; PSPA7_4205 .
GeneIDi 5355582.
KEGGi pap:PSPA7_4205.
PATRICi 19830036. VBIPseAer80442_4025.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi QMVYNLH.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci PAER381754:GHMY-4205-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Dodson R.J., Harkins D., Paulsen I.T.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PA7.

Entry informationi

Entry nameiNAPA_PSEA7
AccessioniPrimary (citable) accession number: A6V924
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: September 3, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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