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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei85Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei152Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei181Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei375Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei379Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei485Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei534Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei561Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei797Substrate; via amide nitrogenUniRule annotation1
Binding sitei805Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei822Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:PSPA7_4205
OrganismiPseudomonas aeruginosa (strain PA7)
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000001582 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Tat-type signalUniRule annotationAdd BLAST29
ChainiPRO_100006972230 – 834Periplasmic nitrate reductaseUniRule annotationAdd BLAST805

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6V924
SMRiA6V924
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 974Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 221Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni245 – 249Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni264 – 266Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni511 – 512Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni721 – 730Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6V924-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLTRREFAK ANAAAIAAAA AGLPILVRAS NLVTEADVTS LDWNKAPCRF
60 70 80 90 100
CGTGCSVMVA TRDGQVVATH GDIKAEVNRG INCVKGYFLS KIMYGSDRLT
110 120 130 140 150
RPLLRMKDGK FDKQGEFQPI SWEQAFDIMA EKFKAALKAK GPESVGMFGS
160 170 180 190 200
GQWTVWEGYA ANKLFKAGLR SNNIDPNARH CMASAVMGFM RSFGMDEPMG
210 220 230 240 250
CYDDIEATDS FVLWGSNMAE MHPVLWSRVT DRRLSAPQVK VAVLSTFEHR
260 270 280 290 300
SFELADIPMV FKPQTDLIIL NYIANHIIES GAVNRDFVER HVRFAHGAED
310 320 330 340 350
IGYGLRPDDP LEKKAKNADK ANTWSDIDFN AFAEFVKPYT LERAARESGV
360 370 380 390 400
PAERLKALAE LYAAPRRKVV SFWTMGFNQH TRGVWANNLI YNIHLLTGKI
410 420 430 440 450
SEPGNSPFSL TGQPSACGTA REVGTFSHRL PADLVVTNPK HRETAEKIWK
460 470 480 490 500
VPAGTIQEKV GFHAVQQSRM LKDGVLNVYW TQVSNNMQAG PNVMQEVLPG
510 520 530 540 550
WRNPDNFVIV SDVYPTVSAQ AADLILPSAM WVEKEGAFGN AERRTQFWHQ
560 570 580 590 600
LVKAPGEAKS DLWQLVEFSK RFTTDEVWPA ELLAKAPELK GKTLYEVLFR
610 620 630 640 650
NGQVDRFPAS DLAKGYANDE VDAFGFYIQK GLFEEYAAFG RGHGHDLAPF
660 670 680 690 700
DAYHEARGLR WPVVDGKETR WRYREGYDPY VSKGSGVQFY GYPDKKAIVF
710 720 730 740 750
ALPYEPPAEA PDRDYPFWLA TGRVLEHWHT GSMTARVPEL YKAVPDAVVY
760 770 780 790 800
MHPDDARQLK LRRGSEVRVV SRRGEIRARV ETRGRNKPPQ GLVFVPFFDA
810 820 830
NKLINKVTLD ATDPISKQTD YKKCAVRIEL LNLA
Length:834
Mass (Da):93,510
Last modified:August 21, 2007 - v1
Checksum:i0CAA2E0F04CE8707
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA Translation: ABR83935.1

Genome annotation databases

EnsemblBacteriaiABR83935; ABR83935; PSPA7_4205
KEGGipap:PSPA7_4205

Similar proteinsi

Entry informationi

Entry nameiNAPA_PSEA7
AccessioniPrimary (citable) accession number: A6V924
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health