Reviewed, UniProtKB/Swiss-Prot A6V8H8 (ACEK_PSEA7)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	PSPA7_4009

Organism

Pseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]

Taxonomic identifier

381754 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000315268
Regions
	Nucleotide binding	318 – 324	7	ATP By similarity
Sites
	Active site	374	1	By similarity
	Binding site	339	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6V8H8-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 81E422B81F7C7F14	FASTA	577	66,698
	10 20 30 40 50 60 MVQSAPASEI AALILRGFDA YREQFREITD GARARFEQAQ WQEAQRASAQ RINLYEEKVA 70 80 90 100 110 120 ETVAGLRAGL ADSELLDVER WPIIKSAYIA QIDLRLDDEL AETWFNSIFC GLFSHDNISD 130 140 150 160 170 180 GTMFVHTTRP SLRAHARAPY TRTYRPGGDL RQALEKIFDD YRFDVPYDDR ERDLERIDAL 190 200 210 220 230 240 LHSNLPDWVC KDPDLAIELI GSVFYRNKGA YLVGRLFTPD EQWPLVFPLL HREGHGIQFD 250 260 270 280 290 300 TVITDEAEVS IIFSFTRSYF MVDVPVPAEL VAFLKRLLPG KHLAELYTSI GFYKQGKSEF 310 320 330 340 350 360 YRALINHLAT TDDRFVMAPG VRGMVMSVFT LPGFNTVFKI IKDRFNPSKS VDHATVIQKY 370 380 390 400 410 420 QLVKNHDRVG RLADTQQFAD FRFPVSKFEP ECLAELLEVA PSTVVVEGDV VLIRHCWTER 430 440 450 460 470 480 RMTPLNIYLE NASEAQTREA LNDYGLAIKQ LAAANIFPGD MLLKNFGVTR HGRVVFYDYD 490 500 510 520 530 540 EICYLTEVNF RYIPEPRFPE DEMSSEPWYS VGPNDVFPEE FPRFLFVDLD QRRLFAKLHG 550 560 570 NLYDAKYWQG LQQQIREGKV IDVFPYRRQE TPEQLLK

References

[1]	Dodson R.J., Harkins D., Paulsen I.T. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000744 Genomic DNA. Translation: ABR83341.1.
RefSeq	YP_001349363.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6V8H8.
Genome annotation databases
GeneID	5355168.
GenomeReviews	Gene locus PSPA7_4009 in contig CP000744_GR.
KEGG	pap:PSPA7_4009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_PSEA7

Accession

Primary (citable) accession number: A6V8H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	January 19, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)