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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

PSPA7_3534

Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97SubstrateBy similarity1
Metal bindingi98Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:PSPA7_3534
OrganismiPseudomonas aeruginosa (strain PA7)
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000001582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000138561 – 162Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolaseAdd BLAST162

Interactioni

Subunit structurei

Homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA6V753.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni75 – 78Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

HOGENOMiHOG000252803.
KOiK02553.
OMAiVTIKCHE.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

A6V753-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHYVTPDLCD AYPELVQVVE PMFSNFGGRD AFGGEIVTIK CFEDNSLVKE
60 70 80 90 100
QVDKDGKGKV LVVDGGGSLR RALLGDMLAE KAAKNGWEGI VVYGCIRDVD
110 120 130 140 150
VIAQTELGVQ ALASHPMKTD KRGIGDLNVV VTFGGVTFRP GEFVYADNNG
160
IIVSPQALNM PG
Length:162
Mass (Da):17,362
Last modified:August 21, 2007 - v1
Checksum:i4F8F1B628344D0AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR81960.1.
RefSeqiWP_003152917.1. NC_009656.1.

Genome annotation databases

EnsemblBacteriaiABR81960; ABR81960; PSPA7_3534.
KEGGipap:PSPA7_3534.
PATRICi19828720. VBIPseAer80442_3367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR81960.1.
RefSeqiWP_003152917.1. NC_009656.1.

3D structure databases

ProteinModelPortaliA6V753.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR81960; ABR81960; PSPA7_3534.
KEGGipap:PSPA7_3534.
PATRICi19828720. VBIPseAer80442_3367.

Phylogenomic databases

HOGENOMiHOG000252803.
KOiK02553.
OMAiVTIKCHE.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRRAAH_PSEA7
AccessioniPrimary (citable) accession number: A6V753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.