A6V6S8 (NUOA2_PSEA7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit A 2
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit A 2
NDH-1 subunit A 2
NUO1 2

Gene names

Name:	nuoA2
Ordered Locus Names:	PSPA7_3402

Organism

Pseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]

Taxonomic identifier

381754 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 132	132	NADH-quinone oxidoreductase subunit A 2 HAMAP MF_01394		PRO_0000362735
Regions
	Transmembrane	9 – 29	21	Helical; Potential
	Transmembrane	66 – 86	21	Helical; Potential
	Transmembrane	93 – 113	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A6V6S8 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: FFA40FCF1BD80C5F	FASTA	132	14,592
	10 20 30 40 50 60 MHGILSPGAW AFIAYVLGAV ALCLVMLGLG RVLGGRSHGR AKNLPFESGV DSTGSARLRF 70 80 90 100 110 120 PVKYALVAML FVIFGIEMPF LYLWAVSVRE NGWAGFVEVA LFVSLLLAGL FYLHRVGALD 130 WSPERRRRKP RD

References

[1]	Dodson R.J., Harkins D., Paulsen I.T. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PA7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000744 Genomic DNA. Translation: ABR85462.1.
RefSeq	YP_001348763.1. NC_009656.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6V6S8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5354151.
GenomeReviews	Gene locus PSPA7_3402 in contig CP000744_GR.
KEGG	pap:PSPA7_3402.
PATRIC	19828458. VBIPseAer80442_3241.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0838.
HOGENOM	HBG605540.
OMA	FECGIES.
PhylomeDB	A6V6S8.
ProtClustDB	CLSK866858.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR023043. NAD(P)H_OxRDtase_bac/plastid. IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
KO	K00330.
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA2_PSEA7

Accession

Primary (citable) accession number: A6V6S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program