Reviewed, UniProtKB/Swiss-Prot A6V3A6 (STHA_PSEA7)

Names and origin

Protein names

Recommended name:
    Soluble pyridine nucleotide transhydrogenase
      Short name=STH
    EC=1.6.1.1
Alternative name(s):
    NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Ordered Locus Names:	PSPA7_2169

Organism

Pseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]

Taxonomic identifier

381754 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity.
Catalytic activity	NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247		PRO_1000012561
Regions
	Nucleotide binding	35 – 44	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6V3A6-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: CACD2DBE241B91B3	FASTA	464	51,147
	10 20 30 40 50 60 MAVYNYDVVI LGTGPAGEGA AMNASKYGRK LAVVDSRRVV GGNCTHLGTI PSKALRHSVK 70 80 90 100 110 120 QIIEFNTNPM FRQIGEPRWF SFPDVLKSAD RVISKQVASR TGYYARNRID MFTGTASFVD 130 140 150 160 170 180 ERTVEVVTPS GAVERLVADQ FVIATGSRPY RPSDINFNHP RVYDSDTILS LSHTPRRLII 190 200 210 220 230 240 YGAGVIGCEY ASIFSGLGVL VDLIDTRDQL LSFLDDEISD ALSYHLRNNN VLIRHNEEYE 250 260 270 280 290 300 RVEGLDNGVI LHLKSGKKIK ADALLWCNGR TGNTDKLGLE NVGIKVNSRG QVEVDENYRT 310 320 330 340 350 360 SVSNIFAAGD VIGWPSLASA AYDQGRSAAG NIVESDSWRF VNDVPTGIYT IPEISSIGKN 370 380 390 400 410 420 ESELTAAKIP YEVGKAFFKG MARAQISNEP VGMLKILFHR ETLEILGVHC FGDQASEIVH 430 440 450 460 IGQAIMNQPG ELNTLKYFVN TTFNYPTMAE AYRVAAFDGL NRLF

References

[1]	Dodson R.J., Harkins D., Paulsen I.T. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000744 Genomic DNA. Translation: ABR81099.1.
RefSeq	YP_001347541.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5357967.
GenomeReviews	Gene locus PSPA7_2169 in contig CP000744_GR.
KEGG	pap:PSPA7_2169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A6V3A6. GEGNTIE.
Family and domain databases
HAMAP	MF_00247. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

STHA_PSEA7

Accession

Primary (citable) accession number: A6V3A6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)