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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Pseudomonas aeruginosa (strain PA7)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityUniRule annotation1
Active sitei2NucleophileUniRule annotation1
Metal bindingi303MagnesiumUniRule annotation1
Metal bindingi365MagnesiumUniRule annotation1
Metal bindingi366MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotationImported, Transferase
Biological processPurine biosynthesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotationImported
Ordered Locus Names:PSPA7_2025Imported
OrganismiPseudomonas aeruginosa (strain PA7)Imported
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000001582 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliA6V2W6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 234Glutamine amidotransferase type-2InterPro annotationAdd BLAST233

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili470 – 490Sequence analysisAdd BLAST21

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis, Glutamine amidotransferaseUniRule annotation

Phylogenomic databases

HOGENOMiHOG000033687
KOiK00764
OMAiFRPLCLG

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.10, 2 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

A6V2W6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGIVGK SNVNQALYDA LTVLQHRGQD AAGIVTCHDD KLYLRKDNGL
60 70 80 90 100
VRDVFQQRHM QRLIGSVGIG HVRYPTAGSS SSAEAQPFYV NSPYGITLAH
110 120 130 140 150
NGNLTNVEQL AKEIYESDLR HVNTNSDSEV LLNVFAHELA VRNKLQPTEE
160 170 180 190 200
DIFAAVSGVH DRCVGGYAVV AMITGHGIVG FRDPNAIRPI VFGQRHTENG
210 220 230 240 250
VEYMIASESV ALDVLGFTLI RDLAPGEAVY ITEEGKLYTR QCARAPKYAP
260 270 280 290 300
CIFEHVYLAR PDSIMDGISV YKARLRMGEK LADKILRERP EHDIDVVIPI
310 320 330 340 350
PDTSRTAALE LANRLGVKFR EGFVKNRYIG RTFIMPGQAA RKKSVRQKLN
360 370 380 390 400
AIELEFRGKN VMLVDDSIVR GTTCKQIIQM AREAGAKNVY FCSAAPAVRY
410 420 430 440 450
PNVYGIDMPS AHELIAHNRS TEDVSKLIGA DWLVYQDLPD LIDAVGGGKI
460 470 480 490 500
KIDRFDCAVF DGEYVTGDVN EAYLNRIEQA RNDATKAKSQ AVSAIIDLYN

D
Length:501
Mass (Da):55,385
Last modified:August 21, 2007 - v1
Checksum:i8662A2B440E4937D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA Translation: ABR80710.1
RefSeqiWP_012075063.1, NC_009656.1

Genome annotation databases

EnsemblBacteriaiABR80710; ABR80710; PSPA7_2025
KEGGipap:PSPA7_2025

Similar proteinsi

Entry informationi

Entry nameiA6V2W6_PSEA7
AccessioniPrimary (citable) accession number: A6V2W6
Entry historyiIntegrated into UniProtKB/TrEMBL: August 21, 2007
Last sequence update: August 21, 2007
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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