ID   MAO1_PSEA7              Reviewed;         564 AA.
AC   A6V1V5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   16-JUN-2009, entry version 16.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=PSPA7_1656;
OS   Pseudomonas aeruginosa (strain PA7).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=381754;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Dodson R.J., Harkins D., Paulsen I.T.;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; CP000744; ABR86715.1; -; Genomic_DNA.
DR   RefSeq; YP_001347040.1; -.
DR   GeneID; 5353903; -.
DR   GenomeReviews; CP000744_GR; PSPA7_1656.
DR   KEGG; pap:PSPA7_1656; -.
DR   OMA; A6V1V5; IQDNNET.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    564       NAD-dependent malic enzyme.
FT                                /FTId=PRO_1000069533.
FT   ACT_SITE    102    102       Proton donor (By similarity).
FT   ACT_SITE    173    173       Proton acceptor (By similarity).
FT   METAL       244    244       Divalent metal cation (By similarity).
FT   METAL       245    245       Divalent metal cation (By similarity).
FT   METAL       268    268       Divalent metal cation (By similarity).
FT   BINDING     155    155       NAD (By similarity).
FT   BINDING     268    268       NAD (By similarity).
FT   BINDING     417    417       NAD (By similarity).
FT   SITE        268    268       Important for activity (By similarity).
SQ   SEQUENCE   564 AA;  62497 MW;  B0CE76C8908F8F5C CRC64;
     MTETAKRPLY VPHAGPSLLE MPLLNKGSAF STQERIDFNL QGLLPHNIET IEEQTERAYS
     QYNLCNTDLD RHIFLRSIQD NNETLFFRLL EEHLEEMLPI IYTPTVGQAC QEFSKIYRTH
     RGLFISYPDR ERIDDILRSA TKNNVKIVVV TDSERILGLG DQGIGGMGIP IGKLSLYTAC
     GGISPAYTLP VVLDVGTNNP DLLNDPMYMG WRHERVSGAQ YEEFVDLFIQ AIKRRWPNVL
     LQFEDFAQTN AMPLLERYKD ELCCFNDDIQ GTAAVAVGTL LAACKAKGEK LSEQTVTFVG
     AGSAGCGIAE QVIAAMRLEG LDEAQARRRI FMVDRWGLLT DDMSNLLDFQ QRLAQKRADL
     GAWGGQQGDD LALLEVIRNA RPTVLIGVSG QRGLFSEEVI RELHRHCPQP LVMPLSNPTS
     RVEATPQEIL NWTDGQALVA TGSPFQPVQV GDRRMPIAQC NNAYIFPGIG LGVIAARATR
     VTDGMLMAAA NALANCSPIV TRGEGAVLPA LGDIREVSKR IAVAVAKQAQ AEGKALQTSD
     EVLNEAIEAN FWFPRYRAYR RTSF
//