NAD-dependent malic enzyme - Pseudomonas aeruginosa (strain PA7)

Contribute

Reviewed, UniProtKB/Swiss-Prot A6V1V5 (MAO1_PSEA7)

Last modified November 25, 2008. Version 12. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	PSPA7_1656

Organism

Pseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

564

NAD-dependent malic enzyme

PRO_1000069533

Sites

Active site

1

Proton donor By similarity

Active site

1

Proton acceptor By similarity

Metal binding

1

Divalent metal cation By similarity

Metal binding

1

Divalent metal cation By similarity

Metal binding

1

Divalent metal cation By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Site

1

Important for activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A6V1V5-1 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: B0CE76C8908F8F5C
Show »

564

62,497

        10         20         30         40         50         60 
MTETAKRPLY VPHAGPSLLE MPLLNKGSAF STQERIDFNL QGLLPHNIET IEEQTERAYS 

        70         80         90        100        110        120 
QYNLCNTDLD RHIFLRSIQD NNETLFFRLL EEHLEEMLPI IYTPTVGQAC QEFSKIYRTH 

       130        140        150        160        170        180 
RGLFISYPDR ERIDDILRSA TKNNVKIVVV TDSERILGLG DQGIGGMGIP IGKLSLYTAC 

       190        200        210        220        230        240 
GGISPAYTLP VVLDVGTNNP DLLNDPMYMG WRHERVSGAQ YEEFVDLFIQ AIKRRWPNVL 

       250        260        270        280        290        300 
LQFEDFAQTN AMPLLERYKD ELCCFNDDIQ GTAAVAVGTL LAACKAKGEK LSEQTVTFVG 

       310        320        330        340        350        360 
AGSAGCGIAE QVIAAMRLEG LDEAQARRRI FMVDRWGLLT DDMSNLLDFQ QRLAQKRADL 

       370        380        390        400        410        420 
GAWGGQQGDD LALLEVIRNA RPTVLIGVSG QRGLFSEEVI RELHRHCPQP LVMPLSNPTS 

       430        440        450        460        470        480 
RVEATPQEIL NWTDGQALVA TGSPFQPVQV GDRRMPIAQC NNAYIFPGIG LGVIAARATR 

       490        500        510        520        530        540 
VTDGMLMAAA NALANCSPIV TRGEGAVLPA LGDIREVSKR IAVAVAKQAQ AEGKALQTSD 

       550        560 
EVLNEAIEAN FWFPRYRAYR RTSF

References

[1]	Dodson R.J., Harkins D., Paulsen I.T. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
	CP000744 Genomic DNA. Translation: ABR86715.1.
RefSeq	YP_001347040.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5353903.
GenomeReviews	Gene locus PSPA7_1656 in contig CP000744_GR.
KEGG	pap:PSPA7_1656.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_PSEA7

Accession

Primary (citable) accession number: A6V1V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	November 25, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents