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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

metE

Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.UniRule annotation

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathway: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi648 – 6481ZincUniRule annotation
Metal bindingi650 – 6501ZincUniRule annotation
Metal bindingi733 – 7331ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPAER381754:GHMY-1556-MONOMER.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseUniRule annotation (EC:2.1.1.14UniRule annotation)
Alternative name(s):
Cobalamin-independent methionine synthaseUniRule annotation
Methionine synthase, vitamin-B12 independent isozymeUniRule annotation
Gene namesi
Name:metEUniRule annotation
Ordered Locus Names:PSPA7_1556
OrganismiPseudomonas aeruginosa (strain PA7)
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000001582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7637635-methyltetrahydropteroyltriglutamate--homocysteine methyltransferasePRO_1000017261Add
BLAST

Proteomic databases

PRIDEiA6V1K5.

Structurei

3D structure databases

ProteinModelPortaliA6V1K5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the vitamin-B12 independent methionine synthase family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0620.
HOGENOMiHOG000246221.
KOiK00549.
OMAiFTKFGWV.
OrthoDBiEOG6FFS3G.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

A6V1K5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALAHTLGFP RIGRDRELKK ALEAYWKGEL DQPGLLQVGR ELRRQHWQLQ
60 70 80 90 100
KDAGIELLPV GDFAWYDQVL AHSLAFGVIP ERFRPADGQP TLDTLFAMAR
110 120 130 140 150
GVAQGCCGGA HAQEMTKWFD TNYHYLVPEF TVDQAFSLSW TQLFEEVDEA
160 170 180 190 200
LALGHAVKPV LIGPLSYLWL GKAKGGEFDR LELLERLLPV YGEIFQGLAA
210 220 230 240 250
RGVEWVQIDE PILVLDLPQA WKNAFERAYN LIQREPLKKL VATYFGGLED
260 270 280 290 300
NLGLAAGLPV DGLHIDLVRA PGQYPTILDR LPAYKVLSLG LVNGRNVWRC
310 320 330 340 350
DLEKALEVLA HAHERLGERL WVAPSCSLLH SPVDLCREDQ LDEELQSWLA
360 370 380 390 400
FAVQKCEEVA ILARALVEPE APEVRQAFEV SRRVEASRRR STRIHKREVQ
410 420 430 440 450
ARLAAVRPQD SRRASAFAER AVQQRARLEL PAFPTTTIGS FPQTSAIRLA
460 470 480 490 500
RQSWKQGRLS QAEYTEAMHS EIRHAVQVQE RLGLDVLVHG EAERNDMVEY
510 520 530 540 550
FAEQLDGYAF TRFGWVQSYG SRCVKPAVIY GDLSRPRPMT VEWIRYAQSL
560 570 580 590 600
TGKVMKGMLT GPVTMLMWSF PREDVSREVQ ARQLALALRD EVSDLEQAGI
610 620 630 640 650
RIVQIDEAAF REGLPLRRAD WPHYLEWATE AFRLCASGVR DETQIHTHMC
660 670 680 690 700
YSEFNDVIES IAAMDADVIT IETSRSDMEL LEAFEAFAYP NEIGPGVYDI
710 720 730 740 750
HSPRVPAVED MVKLLSKAAE RIPAARLWVN PDCGLKTRAW AETEAALANM
760
VAAARQLRQA NLA
Length:763
Mass (Da):86,085
Last modified:August 21, 2007 - v1
Checksum:i7F5BA32D334ED4FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR84681.1.
RefSeqiWP_012074732.1. NC_009656.1.
YP_001346940.1. NC_009656.1.

Genome annotation databases

EnsemblBacteriaiABR84681; ABR84681; PSPA7_1556.
KEGGipap:PSPA7_1556.
PATRICi19824923. VBIPseAer80442_1498.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR84681.1.
RefSeqiWP_012074732.1. NC_009656.1.
YP_001346940.1. NC_009656.1.

3D structure databases

ProteinModelPortaliA6V1K5.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA6V1K5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR84681; ABR84681; PSPA7_1556.
KEGGipap:PSPA7_1556.
PATRICi19824923. VBIPseAer80442_1498.

Phylogenomic databases

eggNOGiCOG0620.
HOGENOMiHOG000246221.
KOiK00549.
OMAiFTKFGWV.
OrthoDBiEOG6FFS3G.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.
BioCyciPAER381754:GHMY-1556-MONOMER.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Dodson R.J., Harkins D., Paulsen I.T.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PA7.

Entry informationi

Entry nameiMETE_PSEA7
AccessioniPrimary (citable) accession number: A6V1K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: June 24, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.