Reviewed, UniProtKB/Swiss-Prot A6V1B5 (PLSB_PSEA7)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	PSPA7_1466

Organism

Pseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]

Taxonomic identifier

381754 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 834	834	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049442
Regions
	Motif	309 – 314	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A6V1B5-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: EF3FBD9719FBA8AE	FASTA	834	94,818
	10 20 30 40 50 60 MPRYPFRRFG FGALRRLLYL WVRSETINQS AFTLKIDRSK PVLYVLQQPS VSDLAVVDTE 70 80 90 100 110 120 CRKAGLPRPV MPVAVGDAIE PAAFFYLTPE PDWLGRQDKR GASPTLVRML AAVGQNGLDD 130 140 150 160 170 180 AQIIPVSVFW GQSPDSESSP WKLLFADNWA VTGRLRKLAR ILILGRKTRV QFSAPIHLRE 190 200 210 220 230 240 LVEQGKGHER TLRMVNRILR VHFRNLKTAV IGPDLSHRRN LVKGLLRAPL VRQAISEECE 250 260 270 280 290 300 SERISQEKAE GIALRYANEI ASDFSYPVIR FLEVILSWFW NKLYEGVKVN HIERVQDVAQ 310 320 330 340 350 360 GNEIVYVPCH RSHIDYLLLS YLLFRNGLTP PHIAAGINLN MPVIGSILRR GGAFFMRRSF 370 380 390 400 410 420 KGNQLYTAVF NEYLHTLFSR GFSTEYFVEG GRSRTGRMLH PRTGMLAITL RSFLRDSRRP 430 440 450 460 470 480 IVFVPVYIGY ERVLEGRTYL GELRGATKKK ESIFDLFKVV GALKQRFGQV WVNFGEPIHL 490 500 510 520 530 540 DQFLDRHQPD WQDQDLGPEY RPDWLPQTTN LLAKDVARHL NDAAAINPVN LVALALLSTS 550 560 570 580 590 600 RQALDESALA RILDLYLALL RQVPYSPSAT LPDGDGQALI EYVKSMNLLA EQKDALGRIL 610 620 630 640 650 660 YLDEQNAVLA TYYRNNVLHV FALPALIASF FQSNSRISRE QLLRFARALY PYLQAELFIR 670 680 690 700 710 720 WSLDELDAVI DQWLAALVEQ GLLRQENDTF IRPAPSSRQY VLLILLARSV TQTLQRFYMA 730 740 750 760 770 780 IALLLNAGQN ALTAEELENL CTVMAQRLSI LHGLNAPEFF DKSLFRHFIQ TLLDLRVLRK 790 800 810 820 830 DETGKLSYHE LLGELAEGAA KRVLPAEIRL SIRQVALERP AEEAAAESND AATN

References

[1]	Dodson R.J., Harkins D., Paulsen I.T. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000744 Genomic DNA. Translation: ABR84229.1.
RefSeq	YP_001346850.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6V1B5.
Genome annotation databases
GeneID	5356576.
GenomeReviews	Gene locus PSPA7_1466 in contig CP000744_GR.
KEGG	pap:PSPA7_1466.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VPVYIGY.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_PSEA7

Accession

Primary (citable) accession number: A6V1B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)