A6V0C9 (MIAB_PSEA7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
EC=2.-.-.-

Alternative name(s):
tRNA-i(6)A37 methylthiotransferase

Gene names

Name:	miaB
Ordered Locus Names:	PSPA7_1128

Organism

Pseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]

Taxonomic identifier

381754 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01864.
Sequence similarities	Belongs to the methylthiotransferase family. MiaB subfamily. Contains 1 MTTase N-terminal domain. Contains 1 TRAM domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP-Rule MF_01864		PRO_0000374462
Regions
	Domain	3 – 120	118	MTTase N-terminal
	Domain	378 – 442	65	TRAM
Sites
	Metal binding	12	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	157	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	161	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	164	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6V0C9 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 7870EB934C946664	FASTA	446	49,975
	10 20 30 40 50 60 MAKKLFIETH GCQMNEYDSS RMADLLGEHQ ALEVTENAAE ADVILLNTCS IREKAQEKVF 70 80 90 100 110 120 SKLGMWRDLK QQNPDLVIGV GGCVASQEGA AIRERAPYVD VVFGPQTLHR LPEMIDAARS 130 140 150 160 170 180 TRKPQVDVSF PEIEKFDRLP EPRVDGPTAF VSVMEGCSKY CSFCVVPYTR GEEVSRPFDD 190 200 210 220 230 240 VIAEVIHLAE NGVREVTLLG QNVNGFRGQT HDGRLADFAE LLRVVAAVDG IERIRYTTSH 250 260 270 280 290 300 PLEFSDALIQ AHAEVPELVK FIHLPVQSGS DRVLAAMKRN HTVLEYKSRI RKLKAAVPDI 310 320 330 340 350 360 CISSDFIVGF PGETEKDFEQ TMKLVEEVGF DFSFSFIYSA RPGTPAADLA DDLPEEVKKR 370 380 390 400 410 420 RLQILQGRIH QQGYEISRRM VGSTQRILVT DFSKKDPGML QGRTENNRIV NFRCDNPRLI 430 440 GQFAQVHIDD ALPHSLRGTL IEGTLH

References

[1]	Dodson R.J., Harkins D., Paulsen I.T. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PA7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000744 Genomic DNA. Translation: ABR81982.1.
RefSeq	YP_001346514.1. NC_009656.1.
3D structure databases
ProteinModelPortal	A6V0C9.
ModBase	Search...
Protein-protein interaction databases
STRING	381754.PSPA7_1128.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABR81982; ABR81982; PSPA7_1128.
GeneID	5359430.
KEGG	pap:PSPA7_1128.
PATRIC	19824070. VBIPseAer80442_1073.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0621.
HOGENOM	HOG000224767.
KO	K06168.
OMA	GIDRIRY.
ProtClustDB	PRK14325.
Enzyme and pathway databases
BioCyc	PAER381754:GHMY-1193-MONOMER.
Family and domain databases
Gene3D	3.80.30.20. 1 hit.
HAMAP	MF_01864. tRNA_metthiotr_MiaB.
InterPro	IPR006638. Elp3/MiaB/NifB. IPR023970. MeThioTfrase/rSAM. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR006463. MiaB_methiolase. IPR007197. rSAM. IPR023404. rSAM_horseshoe. IPR002792. TRAM_dom. [Graphical view]
PANTHER	PTHR11918. PTHR11918. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF01938. TRAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01574. miaB-methiolase. 1 hit. TIGR00089. TIGR00089. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. PS50926. TRAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIAB_PSEA7

Accession

Primary (citable) accession number: A6V0C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program