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A6V0C9 (MIAB_PSEA7) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

EC=2.8.4.3
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
tRNA-i(6)A37 methylthiotransferase
Gene names
Name:miaB
Ordered Locus Names:PSPA7_1128
OrganismPseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]
Taxonomic identifier381754 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864

Catalytic activity

N(6)-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine. HAMAP-Rule MF_01864

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01864

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01864.

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

transferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase HAMAP-Rule MF_01864
PRO_0000374462

Regions

Domain3 – 120118MTTase N-terminal
Domain378 – 44265TRAM

Sites

Metal binding121Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity
Metal binding1571Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1611Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1641Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
A6V0C9 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 7870EB934C946664

FASTA44649,975
        10         20         30         40         50         60 
MAKKLFIETH GCQMNEYDSS RMADLLGEHQ ALEVTENAAE ADVILLNTCS IREKAQEKVF 

        70         80         90        100        110        120 
SKLGMWRDLK QQNPDLVIGV GGCVASQEGA AIRERAPYVD VVFGPQTLHR LPEMIDAARS 

       130        140        150        160        170        180 
TRKPQVDVSF PEIEKFDRLP EPRVDGPTAF VSVMEGCSKY CSFCVVPYTR GEEVSRPFDD 

       190        200        210        220        230        240 
VIAEVIHLAE NGVREVTLLG QNVNGFRGQT HDGRLADFAE LLRVVAAVDG IERIRYTTSH 

       250        260        270        280        290        300 
PLEFSDALIQ AHAEVPELVK FIHLPVQSGS DRVLAAMKRN HTVLEYKSRI RKLKAAVPDI 

       310        320        330        340        350        360 
CISSDFIVGF PGETEKDFEQ TMKLVEEVGF DFSFSFIYSA RPGTPAADLA DDLPEEVKKR 

       370        380        390        400        410        420 
RLQILQGRIH QQGYEISRRM VGSTQRILVT DFSKKDPGML QGRTENNRIV NFRCDNPRLI 

       430        440 
GQFAQVHIDD ALPHSLRGTL IEGTLH 

« Hide

References

[1]Dodson R.J., Harkins D., Paulsen I.T.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PA7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000744 Genomic DNA. Translation: ABR81982.1.
RefSeqYP_001346514.1. NC_009656.1.

3D structure databases

ProteinModelPortalA6V0C9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381754.PSPA7_1128.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR81982; ABR81982; PSPA7_1128.
GeneID5359430.
KEGGpap:PSPA7_1128.
PATRIC19824070. VBIPseAer80442_1073.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0621.
HOGENOMHOG000224767.
KOK06168.
OMAIEVYKDT.
OrthoDBEOG6P5ZD8.

Enzyme and pathway databases

BioCycPAER381754:GHMY-1128-MONOMER.

Family and domain databases

Gene3D3.80.30.20. 1 hit.
HAMAPMF_01864. tRNA_metthiotr_MiaB.
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERPTHR11918. PTHR11918. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIAB_PSEA7
AccessionPrimary (citable) accession number: A6V0C9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families