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A6V0C9

- MIAB_PSEA7

UniProt

A6V0C9 - MIAB_PSEA7

Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

    Catalytic activityi

    N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi12 – 121Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi157 – 1571Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi161 – 1611Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi164 – 1641Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. transferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPAER381754:GHMY-1128-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
    Alternative name(s):
    (Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
    tRNA-i(6)A37 methylthiotransferaseUniRule annotation
    Gene namesi
    Name:miaBUniRule annotation
    Ordered Locus Names:PSPA7_1128
    OrganismiPseudomonas aeruginosa (strain PA7)
    Taxonomic identifieri381754 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000001582: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 446446tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000374462Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi381754.PSPA7_1128.

    Structurei

    3D structure databases

    ProteinModelPortaliA6V0C9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 120118MTTase N-terminalUniRule annotationAdd
    BLAST
    Domaini378 – 44265TRAMUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
    Contains 1 MTTase N-terminal domain.UniRule annotation
    Contains 1 TRAM domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0621.
    HOGENOMiHOG000224767.
    KOiK06168.
    OMAiIEVYKDT.
    OrthoDBiEOG6P5ZD8.

    Family and domain databases

    Gene3Di3.80.30.20. 1 hit.
    HAMAPiMF_01864. tRNA_metthiotr_MiaB.
    InterProiIPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view]
    PANTHERiPTHR11918. PTHR11918. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
    PROSITEiPS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6V0C9-1 [UniParc]FASTAAdd to Basket

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    MAKKLFIETH GCQMNEYDSS RMADLLGEHQ ALEVTENAAE ADVILLNTCS    50
    IREKAQEKVF SKLGMWRDLK QQNPDLVIGV GGCVASQEGA AIRERAPYVD 100
    VVFGPQTLHR LPEMIDAARS TRKPQVDVSF PEIEKFDRLP EPRVDGPTAF 150
    VSVMEGCSKY CSFCVVPYTR GEEVSRPFDD VIAEVIHLAE NGVREVTLLG 200
    QNVNGFRGQT HDGRLADFAE LLRVVAAVDG IERIRYTTSH PLEFSDALIQ 250
    AHAEVPELVK FIHLPVQSGS DRVLAAMKRN HTVLEYKSRI RKLKAAVPDI 300
    CISSDFIVGF PGETEKDFEQ TMKLVEEVGF DFSFSFIYSA RPGTPAADLA 350
    DDLPEEVKKR RLQILQGRIH QQGYEISRRM VGSTQRILVT DFSKKDPGML 400
    QGRTENNRIV NFRCDNPRLI GQFAQVHIDD ALPHSLRGTL IEGTLH 446
    Length:446
    Mass (Da):49,975
    Last modified:August 21, 2007 - v1
    Checksum:i7870EB934C946664
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000744 Genomic DNA. Translation: ABR81982.1.
    RefSeqiYP_001346514.1. NC_009656.1.

    Genome annotation databases

    EnsemblBacteriaiABR81982; ABR81982; PSPA7_1128.
    GeneIDi5359430.
    KEGGipap:PSPA7_1128.
    PATRICi19824070. VBIPseAer80442_1073.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000744 Genomic DNA. Translation: ABR81982.1 .
    RefSeqi YP_001346514.1. NC_009656.1.

    3D structure databases

    ProteinModelPortali A6V0C9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 381754.PSPA7_1128.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR81982 ; ABR81982 ; PSPA7_1128 .
    GeneIDi 5359430.
    KEGGi pap:PSPA7_1128.
    PATRICi 19824070. VBIPseAer80442_1073.

    Phylogenomic databases

    eggNOGi COG0621.
    HOGENOMi HOG000224767.
    KOi K06168.
    OMAi IEVYKDT.
    OrthoDBi EOG6P5ZD8.

    Enzyme and pathway databases

    BioCyci PAER381754:GHMY-1128-MONOMER.

    Family and domain databases

    Gene3Di 3.80.30.20. 1 hit.
    HAMAPi MF_01864. tRNA_metthiotr_MiaB.
    InterProi IPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view ]
    PANTHERi PTHR11918. PTHR11918. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
    PROSITEi PS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Dodson R.J., Harkins D., Paulsen I.T.
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PA7.

    Entry informationi

    Entry nameiMIAB_PSEA7
    AccessioniPrimary (citable) accession number: A6V0C9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3