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A6V0C2 (SYL_PSEA7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PSPA7_1121
OrganismPseudomonas aeruginosa (strain PA7) [Complete proteome] [HAMAP]
Taxonomic identifier381754 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length873 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 873873Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009397

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif632 – 6365"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6V0C2 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: E8C80ECEC74BDAAE

FASTA87397,756
        10         20         30         40         50         60 
MHEQYQPLEI ETQAQNYWKE HQSFLVRELP DKEKFYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYHRMQG RNVLQPMGWD AFGMPAENAA MKNNVAPAAW TYDNIAYMKS QLDSLGLAID 

       130        140        150        160        170        180 
WTREVTTCKP DYYRWEQWLF TRLFEKGVIY RKNGTVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALIEKREIPM YYFKITAYAE ELLESLDNLP GWPEQVKTMQ RNWIGKSRGM EIAFPYDQAS 

       250        260        270        280        290        300 
IGHAGQLKVF TTRPDTLMGA TYVAVAAEHP LATQAAQNDP QLQAFIDECK RGGVAEADIA 

       310        320        330        340        350        360 
TQEKKGLATS LFVEHPLTGD KLPVWVANYV LMNYGEGAVM AVPGHDERDF EFANKYGLPI 

       370        380        390        400        410        420 
RQVIARVEGE NDFEPTVWKE WYGAKDESVR TVNSGKYDDL GYQAAFDAIG ADLEAKGLGQ 

       430        440        450        460        470        480 
ARTQFRLRDW GISRQRYWGC PIPIIHCDAC GDVPVPAEQL PVVLPEDVVP DGAGSPLAKM 

       490        500        510        520        530        540 
PEFYECSCPK CGQPAKRETD TMDTFVESSW YFARYACPQF EGGMLDKKAA DYWLPVDQYI 

       550        560        570        580        590        600 
GGIEHAILHL LYARFFHKLM RDEGLVGSDE PFRNLLTQGM VVADTYYRTT ANGGKDWFNP 

       610        620        630        640        650        660 
ADVEVERDAK AKVVGARLKS DGQPVEIGGT EKMSKSKNNG VDPQSMIDQY GADTCRLFMM 

       670        680        690        700        710        720 
FASPPDMSLE WSDAGVEGAN RFLRRVWRLA HAHVSAGLPG ALDGASLSDA QKQVRRAIHL 

       730        740        750        760        770        780 
AIRQASQDVG QHHKFNTAIA AVMTLMNVLE KAPSQDAQDR ALLQEGLETV VLLLAPITPH 

       790        800        810        820        830        840 
ICHVLWEQLG HAEAVIDARW PVVDESALVQ DTLQLVVQVN GKLRGHIDVA ASASREDVEA 

       850        860        870 
AARANENVLR FTEGLSIRKV IVVPGKLVNI VAN 

« Hide

References

[1]Dodson R.J., Harkins D., Paulsen I.T.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PA7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000744 Genomic DNA. Translation: ABR80652.1.
RefSeqYP_001346507.1. NC_009656.1.

3D structure databases

ProteinModelPortalA6V0C2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381754.PSPA7_1121.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR80652; ABR80652; PSPA7_1121.
GeneID5358943.
KEGGpap:PSPA7_1121.
PATRIC19824056. VBIPseAer80442_1066.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycPAER381754:GHMY-1121-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PSEA7
AccessionPrimary (citable) accession number: A6V0C2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries