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A6V0B4

- LIPA_PSEA7

UniProt

A6V0B4 - LIPA_PSEA7

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Protein

Lipoyl synthase

Gene
lipA, PSPA7_1113
Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi100 – 1001Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi104 – 1041Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPAER381754:GHMY-1113-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:PSPA7_1113
OrganismiPseudomonas aeruginosa (strain PA7)
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000001582: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Lipoyl synthaseUniRule annotationPRO_0000325288Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi381754.PSPA7_1113.

Structurei

3D structure databases

ProteinModelPortaliA6V0B4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A6V0B4-1 [UniParc]FASTAAdd to Basket

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MSTVVEKSGE AKPGKVEVGV KLRGAEKVAR IPVKIIPTEE LPKKPDWIRV    50
RIPVSPEVDR IKQLLRKHKL HSVCEEASCP NLGECFSGGT ATFMIMGDIC 100
TRRCPFCDVG HGRPKPLDVD EPTNLAIAIA DLRLKYVVIT SVDRDDLRDG 150
GAQHFADCLR EIRKLSPGIQ LETLVPDYRG RMDIALEITA NEPPDVFNHN 200
LETVPRLYRS SRPGSDFEWS LDLLQKFKQM VPHVPTKSGL MLGLGETDEE 250
VIEVMQRMRE HDIDMLTLGQ YLQPSRNHLP VQRFVHPDTF AWFAEEGERM 300
GFKNVASGPL VRSSYHADQQ AHGNKIG 327
Length:327
Mass (Da):36,775
Last modified:March 18, 2008 - v2
Checksum:i617D51762CF99F17
GO

Sequence cautioni

The sequence ABR83847.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000744 Genomic DNA. Translation: ABR83847.1. Different initiation.
RefSeqiYP_001346499.1. NC_009656.1.

Genome annotation databases

EnsemblBacteriaiABR83847; ABR83847; PSPA7_1113.
GeneIDi5357989.
KEGGipap:PSPA7_1113.
PATRICi19824042. VBIPseAer80442_1059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000744 Genomic DNA. Translation: ABR83847.1 . Different initiation.
RefSeqi YP_001346499.1. NC_009656.1.

3D structure databases

ProteinModelPortali A6V0B4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 381754.PSPA7_1113.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR83847 ; ABR83847 ; PSPA7_1113 .
GeneIDi 5357989.
KEGGi pap:PSPA7_1113.
PATRICi 19824042. VBIPseAer80442_1059.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci PAER381754:GHMY-1113-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Dodson R.J., Harkins D., Paulsen I.T.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PA7.

Entry informationi

Entry nameiLIPA_PSEA7
AccessioniPrimary (citable) accession number: A6V0B4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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