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A6UYW0

- BIOB_PSEA7

UniProt

A6UYW0 - BIOB_PSEA7

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Protein
Biotin synthase
Gene
bioB, PSPA7_0600
Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi103 – 1031Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi134 – 1341Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi194 – 1941Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi266 – 2661Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPAER381754:GHMY-600-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:PSPA7_0600
OrganismiPseudomonas aeruginosa (strain PA7)
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000001582: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Biotin synthaseUniRule annotation
PRO_0000381552Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi381754.PSPA7_0600.

Structurei

3D structure databases

ProteinModelPortaliA6UYW0.
SMRiA6UYW0. Positions 10-320.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A6UYW0-1 [UniParc]FASTAAdd to Basket

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MSASAAVATR HDWSLAEVRA LFEQPFNDLL FQAQTVHRAY FDPNRVQVST    50
LLSIKTGACP EDCKYCPQSG HYNTGLDKEK LMEVQKVLEA AAEAKAIGST 100
RFCMGAAWKH PSAKDMPYVL EMVKGVKRLG LETCMTLGRL TQEQTQALAD 150
AGLDYYNHNL DTSPEFYGNI ITTRTYGERL QTLAYVREAG MKICSGGILG 200
MGESVDDRAG LLIQLANLPE HPESVPINML VKVKGTPLAE EKDVDPFDFI 250
RTLAVARIMM PKSHVRLSAG REQMNEQMQA LAFMAGANSI FYGEKLLTTK 300
NPQAEKDMQL FARLGIKPEE REEHADEVHQ AAIEQALVEQ RDSQLFYNAA 350
SA 352
Length:352
Mass (Da):39,094
Last modified:August 21, 2007 - v1
Checksum:i486EE6F750E36BE1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000744 Genomic DNA. Translation: ABR84409.1.
RefSeqiYP_001345995.1. NC_009656.1.

Genome annotation databases

EnsemblBacteriaiABR84409; ABR84409; PSPA7_0600.
GeneIDi5356061.
KEGGipap:PSPA7_0600.
PATRICi19823046. VBIPseAer80442_0571.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000744 Genomic DNA. Translation: ABR84409.1 .
RefSeqi YP_001345995.1. NC_009656.1.

3D structure databases

ProteinModelPortali A6UYW0.
SMRi A6UYW0. Positions 10-320.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 381754.PSPA7_0600.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR84409 ; ABR84409 ; PSPA7_0600 .
GeneIDi 5356061.
KEGGi pap:PSPA7_0600.
PATRICi 19823046. VBIPseAer80442_0571.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci PAER381754:GHMY-600-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Dodson R.J., Harkins D., Paulsen I.T.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PA7.

Entry informationi

Entry nameiBIOB_PSEA7
AccessioniPrimary (citable) accession number: A6UYW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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