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Protein

Dihydroxy-acid dehydratase

Gene

ilvD

Organism
Pseudomonas aeruginosa (strain PA7)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi122Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi193Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00047; UER00057.
UPA00049; UER00061.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxy-acid dehydrataseUniRule annotation (EC:4.2.1.9UniRule annotation)
Short name:
DADUniRule annotation
Gene namesi
Name:ilvDUniRule annotation
Ordered Locus Names:PSPA7_0446
OrganismiPseudomonas aeruginosa (strain PA7)
Taxonomic identifieri381754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000001582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000010331 – 612Dihydroxy-acid dehydrataseAdd BLAST612

Family & Domainsi

Sequence similaritiesi

Belongs to the IlvD/Edd family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000173155.
KOiK01687.
OMAiQGRNMAG.

Family and domain databases

HAMAPiMF_00012. IlvD. 1 hit.
InterProiIPR015928. Aconitase/3IPM_dehydase_swvl.
IPR004404. DihydroxyA_deHydtase.
IPR000581. DiOHA_6PGluconate_deHydtase.
IPR020558. DiOHA_6PGluconate_deHydtase_CS.
[Graphical view]
PANTHERiPTHR21000. PTHR21000. 2 hits.
PfamiPF00920. ILVD_EDD. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00110. ilvD. 1 hit.
PROSITEiPS00886. ILVD_EDD_1. 1 hit.
PS00887. ILVD_EDD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6UYF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDYRSKTST HGRNMAGARA LWRATGMKDE DFKKPIIAIA NSFTQFVPGH
60 70 80 90 100
VHLKDLGQLV AREIEKAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSREII
110 120 130 140 150
ADSVEYMVNA HCADAIVCIS NCDKITPGML MAALRLNIPV VFVSGGPMEA
160 170 180 190 200
GKTKLASHGL DLVDAMVVAA DDSCSDEKVA EYERSACPTC GSCSGMFTAN
210 220 230 240 250
SMNCLTEALG LSLPGNGSTL ATHADREQLF LRAGRLAVEL CQRYYGEGDD
260 270 280 290 300
SVLPRNIANF KAFENAMTLD IAMGGSTNTI LHLLAAAQEA EVPFDLRDID
310 320 330 340 350
RLSRKVPQLC KVAPNIQKYH MEDVHRAGGI FSILGELARG GLLHTDVATV
360 370 380 390 400
HSPSMADAIA RWDITQTRDE AVHTFFKAGP AGIPTQTAFS QNTRWPSLDD
410 420 430 440 450
DRAEGCIRSV EHAYSKEGGL AVLYGNIALD GCVVKTAGVD ESIHVFEGRA
460 470 480 490 500
KIFESQDAAV KGILGDEVKA GDIVIIRYEG PKGGPGMQEM LYPTSYLKSK
510 520 530 540 550
GLGKQCALLT DGRFSGGTSG LSIGHASPEA AAGGAIGLVQ DGDKVLIDIP
560 570 580 590 600
NRSINLLVSD EELAARRAEQ DRKGWKPAAP RARRVSTALK AYALLATSAD
610
KGAVRNKALL DG
Length:612
Mass (Da):65,259
Last modified:August 21, 2007 - v1
Checksum:iAFA6338A405F3FE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR86669.1.
RefSeqiWP_012073964.1. NC_009656.1.

Genome annotation databases

EnsemblBacteriaiABR86669; ABR86669; PSPA7_0446.
KEGGipap:PSPA7_0446.
PATRICi19822742. VBIPseAer80442_0419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000744 Genomic DNA. Translation: ABR86669.1.
RefSeqiWP_012073964.1. NC_009656.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR86669; ABR86669; PSPA7_0446.
KEGGipap:PSPA7_0446.
PATRICi19822742. VBIPseAer80442_0419.

Phylogenomic databases

HOGENOMiHOG000173155.
KOiK01687.
OMAiQGRNMAG.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00057.
UPA00049; UER00061.

Family and domain databases

HAMAPiMF_00012. IlvD. 1 hit.
InterProiIPR015928. Aconitase/3IPM_dehydase_swvl.
IPR004404. DihydroxyA_deHydtase.
IPR000581. DiOHA_6PGluconate_deHydtase.
IPR020558. DiOHA_6PGluconate_deHydtase_CS.
[Graphical view]
PANTHERiPTHR21000. PTHR21000. 2 hits.
PfamiPF00920. ILVD_EDD. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00110. ilvD. 1 hit.
PROSITEiPS00886. ILVD_EDD_1. 1 hit.
PS00887. ILVD_EDD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiILVD_PSEA7
AccessioniPrimary (citable) accession number: A6UYF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.