A6UX13 (PURL_META3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Maeo_1459

Organism

Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) [Complete proteome] [HAMAP]

Taxonomic identifier

419665 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050320
Regions
	Nucleotide binding	94 – 105	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A6UX13 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 8344EDA36F1511AC	FASTA	732	79,929
	10 20 30 40 50 60 MDLNDLKFIE EQLGRKPNDV EIGMFENLWS EHCSYRSTKK LLSMFGKTVK ENQNIVVGPG 70 80 90 100 110 120 DDAALIKIDD ETDLCVAMAM ESHNHPSYID PYNGAATGVG GIVRDIISMN AKPIALLNAL 130 140 150 160 170 180 RFGDINGEEK DKVRWLVEGV VDGIRDYGNR IGVPNVGGEC EFDKSYDYNN LVNVVCIGLV 190 200 210 220 230 240 KEGDIVTGKA KETDLTLILV GSTGRDGIGG ASFASKDLTS ESEGDRPSVQ IGDAFTEKCV 250 260 270 280 290 300 IDSTLEACAT KKVKAIKDLG AAGITSSCSE LCYSGGVGAE LYLENVILRD EGMTAYEIMV 310 320 330 340 350 360 SESQERMLLA VEKGSEEEII KIFEKYELPV SIIGKTTDTK RFFVKMNDEV VVDLPLDLLC 370 380 390 400 410 420 EATLTDSEEK ETILETPDNK ENIAEPEPAE LNNILLKLLK SPNINSKKWI YEQYDHEVQL 430 440 450 460 470 480 NTVVRPGKDA SVLRLKEAHP KALALVADCN PTYCKLNPYG GSLNLVCESV RNLATVGAKP 490 500 510 520 530 540 IGMLDNLNFG NPEKPERFYQ IKKCIEGLAD GAEIIGVPVV GGNVSLYNET VIDGKDYPIN 550 560 570 580 590 600 PTPVISIVGT IENINNIPKG AKEGDILIIT APTKDEMGGT EYYKQIHNTE EGIVPKANLE 610 620 630 640 650 660 VEKEIYSKVC ELVNNGLINE AVDCSKGGLG VAISKLCMTN NIGVELDLGD YNTNGLRNDI 670 680 690 700 710 720 LLFSESSGRI ILSVDKNNAD KVVKELNGAI IGTVKGNELK IKLNDNELIN LPIEEMKNKY 730 ENAFSEMMGE NI

References

[1]

"Complete sequence of Methanococcus aeolicus Nankai-3." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nankai-3 / ATCC BAA-1280.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000743 Genomic DNA. Translation: ABR57035.1.
RefSeq	YP_001325647.1. NC_009635.1.
3D structure databases
ProteinModelPortal	A6UX13.
ModBase	Search...
Protein-protein interaction databases
STRING	A6UX13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5326931.
GenomeReviews	Gene locus Maeo_1459 in contig CP000743_GR.
KEGG	mae:Maeo_1459.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	MAEO419665:MAEO_1459-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_META3

Accession

Primary (citable) accession number: A6UX13

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program