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Protein

Ketol-acid reductoisomerase (NADP(+))

Gene

ilvC

Organism
Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (Maeo_0682)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (Maeo_0682)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei47NADPUniRule annotation1
Binding sitei52NADPUniRule annotation1
Active sitei107UniRule annotation1
Binding sitei133NADP; via amide nitrogenUniRule annotation1
Metal bindingi190Magnesium 1UniRule annotation1
Metal bindingi190Magnesium 2UniRule annotation1
Metal bindingi194Magnesium 1UniRule annotation1
Metal bindingi226Magnesium 2UniRule annotation1
Metal bindingi230Magnesium 2UniRule annotation1
Binding sitei251SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 27NADPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseImported, OxidoreductaseUniRule annotationImported
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

BioCyciMAEO419665:G1G9A-1199-MONOMER
UniPathwayiUPA00047; UER00056
UPA00049; UER00060

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)
Short name:
KARIUniRule annotation
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Short name:
AHIRUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
Ordered Locus Names:Maeo_1175Imported
OrganismiMethanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)Imported
Taxonomic identifieri419665 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi

Interactioni

Protein-protein interaction databases

STRINGi419665.Maeo_1175

Structurei

3D structure databases

ProteinModelPortaliA6UW80
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 181KARI N-terminal RossmannInterPro annotationAdd BLAST181
Domaini182 – 327KARI C-terminal knottedInterPro annotationAdd BLAST146

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04465 Archaea
COG0059 LUCA
HOGENOMiHOG000016230
KOiK00053
OMAiRAMFSWL
OrthoDBiPOG093Z0546

Family and domain databases

HAMAPiMF_00435 IlvC, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013023 KARI
IPR000506 KARI_C
IPR013116 KARI_N
IPR014359 KARI_prok
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR21371 PTHR21371, 1 hit
PfamiView protein in Pfam
PF01450 IlvC, 1 hit
PF07991 IlvN, 1 hit
PIRSFiPIRSF000116 IlvC_gammaproteo, 2 hits
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00465 ilvC, 1 hit
PROSITEiView protein in PROSITE
PS51851 KARI_C, 1 hit
PS51850 KARI_N, 1 hit

Sequencei

Sequence statusi: Complete.

A6UW80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVYYEEDAN YDAVKDKTIA VIGYGSQGMA QSCNMKDSGL NVIVGLRPNG
60 70 80 90 100
ASWEKAKADG HTVMSVEEAA EKADIIHILI PDEVQKDVYN NQIKQHLTEG
110 120 130 140 150
KTLSFSHGYN VHFKYIDPIK GVNVIMVAPK SPGAMVRRTY TEGFGVPGLV
160 170 180 190 200
CVERDETGDA LDIALGMAKA EGLTKAGVIK TTFKEETETD LFGEQAVLCG
210 220 230 240 250
GVTELIKAGF DTLVEAGYSP EMAYFETCNE LKLIVDLIYQ KGLAGMWNDV
260 270 280 290 300
SNTAEYGGFV TRERVINEES RKAMREILKE IQNGKFARDW ALENVSGIPH
310 320
LNAMRRLEDE SLLETTGKKL RKMCGLQKD
Length:329
Mass (Da):36,348
Last modified:August 21, 2007 - v1
Checksum:i8D72B89581B32026
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000743 Genomic DNA Translation: ABR56752.1
RefSeqiWP_011973884.1, NC_009635.1

Genome annotation databases

EnsemblBacteriaiABR56752; ABR56752; Maeo_1175
GeneIDi5326459
KEGGimae:Maeo_1175

Similar proteinsi

Entry informationi

Entry nameiA6UW80_META3
AccessioniPrimary (citable) accession number: A6UW80
Entry historyiIntegrated into UniProtKB/TrEMBL: August 21, 2007
Last sequence update: August 21, 2007
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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