A6UW46 (A6UW46_META3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit HAMAP MF_00324
Short name=Pol II HAMAP MF_00324
EC=2.7.7.7 HAMAP MF_00324

Gene names

Name:	polC HAMAP MF_00324
Ordered Locus Names:	Maeo_1141

Organism

Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) [Complete proteome] [HAMAP] EMBL ABR56718.1

Taxonomic identifier

419665 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	1665 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324 Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family. HAMAP MF_00324

Ontologies

Keywords
Biological process	DNA replication HAMAP MF_00324
Ligand	DNA-binding HAMAP MF_00324
Molecular function	DNA-directed DNA polymerase HAMAP MF_00324 Exonuclease HAMAP MF_00324 Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme HAMAP MF_00324
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A6UW46 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 937E7EBEDD1156CF	FASTA	1,665	190,575
	10 20 30 40 50 60 MVHVKASKEM EFYFNNILEN VKEIYNIAEK CRNKGYDVNN YVEIPLANDM ADRVEGIVGP 70 80 90 100 110 120 KNVSGRIREL VIELGKEPAA LEVAKEIVEG KFGTDSPEVL AEQAVRTALA IITEGIVAAP 130 140 150 160 170 180 LEGIAGVKIK TNKDNSKYLA IYFAGPIRSA GGTAQALAVL VGDYVRKNMG LNKYIPSEDE 190 200 210 220 230 240 IERYVEEVDL YQSEVGGFQY SPSPDEIRTA VKNISIEITG ETTDDVEVSG HRDLDRVETN 250 260 270 280 290 300 CIRGGALLAL VEGVLLKSPK ILRHVDKLGM EGWDWLKQLK EKKKDEDEEK SDELKGEDEN 310 320 330 340 350 360 TEFTDEELEE YHKIYDDVEI EAVTKFIGDV IAGRPVFAHP STNGGFRLRY GRSRNTGFAT 370 380 390 400 410 420 DGFHPALMYL VDEFMAIGTQ LKTERPGKAT CVVPVDSIEP PIAKLKNGTV LKIDTIEKAI 430 440 450 460 470 480 EYRKSVEEIL FLGDILVNYG DFLENNHNLV PSSWCEEWYE KILISKNIKY TDEFINNPTP 490 500 510 520 530 540 KEATIYALNT NTPLHPKYTY YWHDVSKEDI LQLRNWLLTG KEEIIDNNNN NNNNNNNNFD 550 560 570 580 590 600 AINSSWKIQY NNKDENNIKS KRILELIGCP HDVIYSENKE QYIIIDEYYP LLYSLGYDIE 610 620 630 640 650 660 NNKDLLENQN LEEKCQKSKN PIHFINLLSQ FEIKRKAYIY IGARMGRPEK AAPRKMKPPV 670 680 690 700 710 720 NSLFPIGNAG GVVRLINKAV ETNTTDNIEL YSGICPNCGK LGLYNKCLFC GAPVKLSGPT 730 740 750 760 770 780 TVELPIKDYW YKSLENLKIN KPGNVKCIKG MTSKDKIVEP LEKGILRAVN DVFVFKDGTL 790 800 810 820 830 840 RYDCTDVPLT HFKPSEINIS IEKLKELGYD KDIRGAPIEH PDQIIELKIQ DVIIPNNCAE 850 860 870 880 890 900 YFIRATKFID DLLEKFYKKD RFYNVKEKDD LIGHLVIGMA PHTSAGMVGR IIGYCNAYVG 910 920 930 940 950 960 YAHPYFHASK RRNCFPPNTT ILVNINGEVK RITIEELYNL YDNEYYKNMA YIKKDIKNNN 970 980 990 1000 1010 1020 NIKVYSFDTL NKKMVLTDIE EVLKIQSPNH LVNIGLDTGR SFETTPDHPV MVYDEKEDKF 1030 1040 1050 1060 1070 1080 IKKNAMDVEE NDLMLIPKLD FEEDEEQEQD NIEHIDLLAE LSKEEYKNMW ELLRIRGISD 1090 1100 1110 1120 1130 1140 WIKNNIQKHC LKEKNENGKE NLIKNIAKYI KQDTIPLNLL LKILKNAELN ITDVPKDAFI 1150 1160 1170 1180 1190 1200 AVRRDKVNIR RIIKIEPLLK IIGYYLAEGY MRKTSSVYQI NFSNYDEEVI KDIKNSLNEA 1210 1220 1230 1240 1250 1260 FGDGFGIYEK DGKITVGSRI IYLLFTEILK TGINAHNKRV PSFIFKLPKE KVKLMLSAYF 1270 1280 1290 1300 1310 1320 TGDGSAVKTR PIVVIYSANK KLLEDIDTLM ISKFGLYGNW GADKNANGRK GNVVMKYHEK 1330 1340 1350 1360 1370 1380 RETEIPKSTV YRIDYNGIQA MKYFENIGFT SSKKQNIYEL HTHKNFKAKK GLKEYGYIVK 1390 1400 1410 1420 1430 1440 VRNKSIIKAE DEFVYSLNAK KYHNVVINSN IQMHNCDGDE DGIFLLLDAF LNFSKRFLPD 1450 1460 1470 1480 1490 1500 KRGGQMDAPL VLTTLLDPKE VDGEVHNLDT MWEYPLEFYE KTLEMPTPKD IKELIETVED 1510 1520 1530 1540 1550 1560 RLDKPSQYEK LGYTHETTDI SNGPLICAYK TLGSMLEKTD AQLTVARKIR ATYERDVAEK 1570 1580 1590 1600 1610 1620 VIQSHFVPDL IGNLRAFSRQ GFRCKCGAKY RRVPLKGVCR KCGSKLILTV SKGAVEKYMG 1630 1640 1650 1660 VSQTMAEKYN ASDYVKQRLE LIKEGIDNLF QSDKRKQVKI EDFFK

References

[1]

"Complete sequence of Methanococcus aeolicus Nankai-3." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nankai-3 / ATCC BAA-1280.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000743 Genomic DNA. Translation: ABR56718.1.
RefSeq	YP_001325330.1. NC_009635.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6UW46.
Protein family/group databases
MEROPS	N10.005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5327666.
GenomeReviews	Gene locus Maeo_1141 in contig CP000743_GR.
KEGG	mae:Maeo_1141.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04008.
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK14715.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01445. Intein_Nterm. 1 hit. TIGR00354. PolC. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A6UW46_META3

Accession

Primary (citable) accession number: A6UW46

Entry history

Integrated into UniProtKB/TrEMBL:	August 21, 2007
Last sequence update:	August 21, 2007
Last modified:	November 16, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)