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A6UVK2

- SYI_META3

UniProt

A6UVK2 - SYI_META3

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei608 – 6081ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMAEO419665:GHVZ-957-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Maeo_0945
OrganismiMethanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280)
Taxonomic identifieri419665 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000001106: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10401040Isoleucine--tRNA ligasePRO_1000022150Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi419665.Maeo_0945.

Structurei

3D structure databases

ProteinModelPortaliA6UVK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRYVEGKW.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6UVK2 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEIKGKVDF REMDKEIKEF WENNEIYQKV KKLNENYPDY YFVDGPPYCS
60 70 80 90 100
GSIHLGTAWN KTIKDTVLRF KRMQNYNVLD KAGWDMHGLP IEVKVEHEFN
110 120 130 140 150
LQSKKDIETK VGTDVFIEKC KEFALNNKEV MENQFKNLGI WLDWENAYLP
160 170 180 190 200
IKNDYIEAGW WSLKRAHEKE LLSKDLRVGY WCPRCETSLA EHEVRGEYQD
210 220 230 240 250
VLDPSVYVKF KVADDNSYPN TYFVIWTTTP WTLISNLLIA VNPEFDYGFV
260 270 280 290 300
EVIFDNERKE TWVIAEALVE AVIKLAKKQN NIKSYNIVKK VKGKELEGIK
310 320 330 340 350
YVPALLEENE RQKEFFQLEK VHTIVLGEHV SLDGGTGLVH TAPGFGEEDF
360 370 380 390 400
EVGKKYNSPI YSPIDDEGKY VEGKWKGVFV KDADEDIIAT LTDKNLIVNA
410 420 430 440 450
GKKKHTYPHC WRCKTPLLFR STEQWFLNIS KIKNNIVEHA KNTDWVPNWV
460 470 480 490 500
ETRYINGVKF VGDWNISRQR YWGIPLPIWI CENKDCGKYK IIGSVEELKQ
510 520 530 540 550
EMINDIPIND DLHKPTVDKI IMKCDCGCEM KRTPDVLDVW YDSGLAPYAS
560 570 580 590 600
INAKELKKAD FIVEGNDQVT KWFYSQHALS EIVFDDIPYK KCMMHGFTLD
610 620 630 640 650
ETGEKMSKSI GNVVNPDDVV EEFGADLLRF YLLSANKAWE DLRFSIGEMN
660 670 680 690 700
DVKSVFNTLW NSYSFAVNYM VLDDFSPNEE YFNYLRDEDK WIISKINSLT
710 720 730 740 750
KEAIEVLEIP HLHEYTWKVR DFILNDLSRW YIKLIRNRTW MEKEDPDKLS
760 770 780 790 800
AYQTLYYVLM KLVVILAPVA PHISEKIYQN LKIEGMPQSI FMTKIVVEEE
810 820 830 840 850
YINKEVEEGI ETAREIVDAI LKGRDKVKYT LRYPISKIIL PNELGDIVNK
860 870 880 890 900
YHYIIKEQGN VKNIEVKEFE GNISLKPNFR TLGASFKSDV PAVVKILNSQ
910 920 930 940 950
NPKELKDKLA EGEITIDGFT IKPEHVEFKI DIPDNIVGME LRKGSVYIDI
960 970 980 990 1000
ELTEEIIKDG LKREVIRRIQ SMRKDMDLDI EEKIKITMEG IEFNEDALKD
1010 1020 1030 1040
IEKEVRGTFE PTIECDNIQE WNIKTPNGEV YNLKIGVKKN
Length:1,040
Mass (Da):121,325
Last modified:August 21, 2007 - v1
Checksum:i21BA4C114FCB46B2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000743 Genomic DNA. Translation: ABR56524.1.
RefSeqiYP_001325136.1. NC_009635.1.

Genome annotation databases

EnsemblBacteriaiABR56524; ABR56524; Maeo_0945.
GeneIDi5327661.
KEGGimae:Maeo_0945.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000743 Genomic DNA. Translation: ABR56524.1 .
RefSeqi YP_001325136.1. NC_009635.1.

3D structure databases

ProteinModelPortali A6UVK2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 419665.Maeo_0945.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR56524 ; ABR56524 ; Maeo_0945 .
GeneIDi 5327661.
KEGGi mae:Maeo_0945.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RYVEGKW.

Enzyme and pathway databases

BioCyci MAEO419665:GHVZ-957-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nankai-3 / ATCC BAA-1280.

Entry informationi

Entry nameiSYI_META3
AccessioniPrimary (citable) accession number: A6UVK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3