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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei608ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMAEO419665:G1G9A-955-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Maeo_0945
OrganismiMethanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)
Taxonomic identifieri419665 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221501 – 1040Isoleucine--tRNA ligaseAdd BLAST1040

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi419665.Maeo_0945

Structurei

3D structure databases

ProteinModelPortaliA6UVK2
SMRiA6UVK2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 57"HIGH" regionAdd BLAST11
Motifi605 – 609"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A6UVK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEIKGKVDF REMDKEIKEF WENNEIYQKV KKLNENYPDY YFVDGPPYCS
60 70 80 90 100
GSIHLGTAWN KTIKDTVLRF KRMQNYNVLD KAGWDMHGLP IEVKVEHEFN
110 120 130 140 150
LQSKKDIETK VGTDVFIEKC KEFALNNKEV MENQFKNLGI WLDWENAYLP
160 170 180 190 200
IKNDYIEAGW WSLKRAHEKE LLSKDLRVGY WCPRCETSLA EHEVRGEYQD
210 220 230 240 250
VLDPSVYVKF KVADDNSYPN TYFVIWTTTP WTLISNLLIA VNPEFDYGFV
260 270 280 290 300
EVIFDNERKE TWVIAEALVE AVIKLAKKQN NIKSYNIVKK VKGKELEGIK
310 320 330 340 350
YVPALLEENE RQKEFFQLEK VHTIVLGEHV SLDGGTGLVH TAPGFGEEDF
360 370 380 390 400
EVGKKYNSPI YSPIDDEGKY VEGKWKGVFV KDADEDIIAT LTDKNLIVNA
410 420 430 440 450
GKKKHTYPHC WRCKTPLLFR STEQWFLNIS KIKNNIVEHA KNTDWVPNWV
460 470 480 490 500
ETRYINGVKF VGDWNISRQR YWGIPLPIWI CENKDCGKYK IIGSVEELKQ
510 520 530 540 550
EMINDIPIND DLHKPTVDKI IMKCDCGCEM KRTPDVLDVW YDSGLAPYAS
560 570 580 590 600
INAKELKKAD FIVEGNDQVT KWFYSQHALS EIVFDDIPYK KCMMHGFTLD
610 620 630 640 650
ETGEKMSKSI GNVVNPDDVV EEFGADLLRF YLLSANKAWE DLRFSIGEMN
660 670 680 690 700
DVKSVFNTLW NSYSFAVNYM VLDDFSPNEE YFNYLRDEDK WIISKINSLT
710 720 730 740 750
KEAIEVLEIP HLHEYTWKVR DFILNDLSRW YIKLIRNRTW MEKEDPDKLS
760 770 780 790 800
AYQTLYYVLM KLVVILAPVA PHISEKIYQN LKIEGMPQSI FMTKIVVEEE
810 820 830 840 850
YINKEVEEGI ETAREIVDAI LKGRDKVKYT LRYPISKIIL PNELGDIVNK
860 870 880 890 900
YHYIIKEQGN VKNIEVKEFE GNISLKPNFR TLGASFKSDV PAVVKILNSQ
910 920 930 940 950
NPKELKDKLA EGEITIDGFT IKPEHVEFKI DIPDNIVGME LRKGSVYIDI
960 970 980 990 1000
ELTEEIIKDG LKREVIRRIQ SMRKDMDLDI EEKIKITMEG IEFNEDALKD
1010 1020 1030 1040
IEKEVRGTFE PTIECDNIQE WNIKTPNGEV YNLKIGVKKN
Length:1,040
Mass (Da):121,325
Last modified:August 21, 2007 - v1
Checksum:i21BA4C114FCB46B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000743 Genomic DNA Translation: ABR56524.1
RefSeqiWP_011973656.1, NC_009635.1

Genome annotation databases

EnsemblBacteriaiABR56524; ABR56524; Maeo_0945
GeneIDi5327661
KEGGimae:Maeo_0945

Entry informationi

Entry nameiSYI_META3
AccessioniPrimary (citable) accession number: A6UVK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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