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A6UVK2

- SYI_META3

UniProt

A6UVK2 - SYI_META3

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei608 – 6081ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMAEO419665:GHVZ-957-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Maeo_0945
    OrganismiMethanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280)
    Taxonomic identifieri419665 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
    ProteomesiUP000001106: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10401040Isoleucine--tRNA ligasePRO_1000022150Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi419665.Maeo_0945.

    Structurei

    3D structure databases

    ProteinModelPortaliA6UVK2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi47 – 5711"HIGH" regionAdd
    BLAST
    Motifi605 – 6095"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRYVEGKW.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6UVK2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKEIKGKVDF REMDKEIKEF WENNEIYQKV KKLNENYPDY YFVDGPPYCS     50
    GSIHLGTAWN KTIKDTVLRF KRMQNYNVLD KAGWDMHGLP IEVKVEHEFN 100
    LQSKKDIETK VGTDVFIEKC KEFALNNKEV MENQFKNLGI WLDWENAYLP 150
    IKNDYIEAGW WSLKRAHEKE LLSKDLRVGY WCPRCETSLA EHEVRGEYQD 200
    VLDPSVYVKF KVADDNSYPN TYFVIWTTTP WTLISNLLIA VNPEFDYGFV 250
    EVIFDNERKE TWVIAEALVE AVIKLAKKQN NIKSYNIVKK VKGKELEGIK 300
    YVPALLEENE RQKEFFQLEK VHTIVLGEHV SLDGGTGLVH TAPGFGEEDF 350
    EVGKKYNSPI YSPIDDEGKY VEGKWKGVFV KDADEDIIAT LTDKNLIVNA 400
    GKKKHTYPHC WRCKTPLLFR STEQWFLNIS KIKNNIVEHA KNTDWVPNWV 450
    ETRYINGVKF VGDWNISRQR YWGIPLPIWI CENKDCGKYK IIGSVEELKQ 500
    EMINDIPIND DLHKPTVDKI IMKCDCGCEM KRTPDVLDVW YDSGLAPYAS 550
    INAKELKKAD FIVEGNDQVT KWFYSQHALS EIVFDDIPYK KCMMHGFTLD 600
    ETGEKMSKSI GNVVNPDDVV EEFGADLLRF YLLSANKAWE DLRFSIGEMN 650
    DVKSVFNTLW NSYSFAVNYM VLDDFSPNEE YFNYLRDEDK WIISKINSLT 700
    KEAIEVLEIP HLHEYTWKVR DFILNDLSRW YIKLIRNRTW MEKEDPDKLS 750
    AYQTLYYVLM KLVVILAPVA PHISEKIYQN LKIEGMPQSI FMTKIVVEEE 800
    YINKEVEEGI ETAREIVDAI LKGRDKVKYT LRYPISKIIL PNELGDIVNK 850
    YHYIIKEQGN VKNIEVKEFE GNISLKPNFR TLGASFKSDV PAVVKILNSQ 900
    NPKELKDKLA EGEITIDGFT IKPEHVEFKI DIPDNIVGME LRKGSVYIDI 950
    ELTEEIIKDG LKREVIRRIQ SMRKDMDLDI EEKIKITMEG IEFNEDALKD 1000
    IEKEVRGTFE PTIECDNIQE WNIKTPNGEV YNLKIGVKKN 1040
    Length:1,040
    Mass (Da):121,325
    Last modified:August 21, 2007 - v1
    Checksum:i21BA4C114FCB46B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000743 Genomic DNA. Translation: ABR56524.1.
    RefSeqiYP_001325136.1. NC_009635.1.

    Genome annotation databases

    EnsemblBacteriaiABR56524; ABR56524; Maeo_0945.
    GeneIDi5327661.
    KEGGimae:Maeo_0945.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000743 Genomic DNA. Translation: ABR56524.1 .
    RefSeqi YP_001325136.1. NC_009635.1.

    3D structure databases

    ProteinModelPortali A6UVK2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 419665.Maeo_0945.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR56524 ; ABR56524 ; Maeo_0945 .
    GeneIDi 5327661.
    KEGGi mae:Maeo_0945.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RYVEGKW.

    Enzyme and pathway databases

    BioCyci MAEO419665:GHVZ-957-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Nankai-3 / ATCC BAA-1280.

    Entry informationi

    Entry nameiSYI_META3
    AccessioniPrimary (citable) accession number: A6UVK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3