Reviewed, UniProtKB/Swiss-Prot A6UVC4 (DNLI_META3)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	Maeo_0864

Organism

Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) [Complete proteome] [HAMAP]

Taxonomic identifier

419665 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	DNA ligase HAMAP MF_00407		PRO_0000365251
Sites
	Active site	254	1	N6-AMP-lysine intermediate By similarity
	Binding site	252	1	ATP By similarity
	Binding site	259	1	ATP By similarity
	Binding site	274	1	ATP By similarity
	Binding site	303	1	ATP By similarity
	Binding site	343	1	ATP By similarity
	Binding site	419	1	ATP By similarity
	Binding site	425	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6UVC4-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 318AA5FA42DABED6	FASTA	562	64,754
	10 20 30 40 50 60 MAILFKEVCD IFYKIESTTK RLEKMEYFIE LIKMVENKKA PQDLKKICQI AVGRVFAEHE 70 80 90 100 110 120 NKELGIGPNI LIDAIISTGI NKKTIQNTIN QTGDIGTALE QLGHNIKQTS LFQTPPTLEE 130 140 150 160 170 180 VYTTLKKLST IEGGQSQKKK IRHISSILIK SSPIEQRYLA RLILEDMRIG MSIPTILGAF 190 200 210 220 230 240 SKYYNQPKEN LEKIYAVVND IGLLAEKLAQ RCDIYNDEEL QLKIFRPIKP MLAQLIGSIN 250 260 270 280 290 300 DAIIEMGAPQ FETKYDGARV QIHKKDNIVK IYSRKLEDIT NSIPEIVEEV KNIEAQNLII 310 320 330 340 350 360 EGECVAMDKN GRPRPFQDIL RRFRRKYNID SIQSEINLKV YVFDILYYNN KSLIELPLIE 370 380 390 400 410 420 RRSILEKILT NKHNKLNISH KLTTDNEQKA REFYEWSLSI GHEGVMIKNP NAIYTPGSRV 430 440 450 460 470 480 RTMYKFKPTL ESLDVVITKA KMGMGKRKEW YGSFEIAVKD YENNLYTIGH VGSGLTEEEL 490 500 510 520 530 540 HNLTEQIKNI TIEVINDEAI VEPKIVLEIS YEEIQESDKY KCGYALRFPR VARIRTDKSI 550 560 EDINSIEDIE RIFDIQKGKG NL

References

[1]

"Complete sequence of Methanococcus aeolicus Nankai-3." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000743 Genomic DNA. Translation: ABR56446.1.
RefSeq	YP_001325058.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6UVC4.
Genome annotation databases
GeneID	5326248.
GenomeReviews	Gene locus Maeo_0864 in contig CP000743_GR.
KEGG	mae:Maeo_0864.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SNLHLGA.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_META3

Accession

Primary (citable) accession number: A6UVC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)