A6UUS4 (NADE_META3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Maeo_0662

Organism

Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) [Complete proteome] [HAMAP]

Taxonomic identifier

419665 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077574
Regions
	Nucleotide binding	29 – 36	8	ATP By similarity
Sites
	Active site	31	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6UUS4 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 61EB54B24D332F56	FASTA	254	27,977
	10 20 30 40 50 60 MNCEELTNKL TNFVLSKVGE ANAKGVVLGL SGGIDSSLVA TICVKALGKD NVLGVIMPEK 70 80 90 100 110 120 NSNTQDKEHA ELLANQLGIK YTVSDITDVL KSFGAGGYIP TKEFDKMADG NLKPRIRMCI 130 140 150 160 170 180 LYYFANKNNL LVAGTSNKSE IYMGYGTKYG DLGSDFLLIG NLFKTEVREL SKYLGVPDEI 190 200 210 220 230 240 INKAPSAGLW EGQTDEKELG ITYELLDKVL MAIEQNKEKE DISKELTVPI EKIEEILNRI 250 ESNKHKSQPI PIPN

References

[1]

"Complete sequence of Methanococcus aeolicus Nankai-3." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nankai-3 / ATCC BAA-1280.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000743 Genomic DNA. Translation: ABR56246.1.
RefSeq	YP_001324858.1. NC_009635.1.
3D structure databases
ProteinModelPortal	A6UUS4.
ModBase	Search...
Protein-protein interaction databases
STRING	A6UUS4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5326908.
GenomeReviews	Gene locus Maeo_0662 in contig CP000743_GR.
KEGG	mae:Maeo_0662.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	DGAVDCH.
Enzyme and pathway databases
BioCyc	MAEO419665:MAEO_0662-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_META3

Accession

Primary (citable) accession number: A6UUS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program