A6UUN3 (SYA_META3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Maeo_0621

Organism

Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) [Complete proteome] [HAMAP]

Taxonomic identifier

419665 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	910 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 910	910	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_1000074505
Sites
	Metal binding	614	1	Zinc By similarity
	Metal binding	618	1	Zinc By similarity
	Metal binding	718	1	Zinc By similarity
	Metal binding	722	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6UUN3 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: FC63B7FF19AB9408	FASTA	910	102,722
	10 20 30 40 50 60 MEIKHDYNVQ LFKEKGFIRK QCKECNQYFW TLDPKRETCG DSPCDEYSFI GSSITNKEYT 70 80 90 100 110 120 YNEMVKEFLN FFDKNGHTPI KRYPVSARRW RDDILLTIAS IAVFQPWVTK GIVKPVANPL 130 140 150 160 170 180 VIAQPCIRLN DIDNVGRTGR HMTCFTMGGH HAFNTDEDFK YWTDRTVELC YNFFTNLGID 190 200 210 220 230 240 GSSITFIESW WEGGGNAGPC YEVITHGVEL ATLVFMQYEK TEQGDYIEMP LKIVDTGYGL 250 260 270 280 290 300 ERFVWASKGT PTVYEAVFGD IIGKLMDDAN INMNDIGPKI LAESATLAGL MDIENVGDLR 310 320 330 340 350 360 ILRQKVAEKL NLDVNELDSI LSPIENIYAI ADHTRCLAFM LGDGIVPSNV KDGYLARLLV 370 380 390 400 410 420 RKTLRYIKNV GLSLSLKDIV AMQLENLKEI YPELMDMKEY IMDVLEEEEN KYIQTITKGK 430 440 450 460 470 480 GAVERIAKSK DEITLDDLIE LYDSKGLPPE VVRDIVEEIN KKGSKNNKGN KTIKITVPDN 490 500 510 520 530 540 FYTIVAERHE EKKEDSKSEK GENTAEKSTI SINLDDIPET ELLFYSNPYQ KEVEAKILKI 550 560 570 580 590 600 IGNVVILDKT VFYPEGGGQK YDIGLIGNKK IISVQKNNNG IVCHTVENTD GLNEEDTIIA 610 620 630 640 650 660 KLNWENRLNL MRNHTATHII NAAASKVLGK HIWQTGSNVE SNKARLDITH YKRITREEIK 670 680 690 700 710 720 EIEKIANQIV LDAIPVKSTV MGRNEAEQKY GFKIYQGGVV PGNILRILDI EGVDVEACGG 730 740 750 760 770 780 THCQNTSEVG YIKILKTERI QDGVERLEYT SGINSVNEVS LMEDILLNAS SIVGVPCENL 790 800 810 820 830 840 PKTVNRFFEE WKEQKKIIEE LHKKIGELEK GNLNDKFEKV GKYDLLVEKV EGAPKELMSI 850 860 870 880 890 900 ADNLVNEKDN SIVILLNNSG YILCKCGEKV EIKMGELLRK IAKGGGKDKM AQGKCADDIE 910 TLKSKVVGEL

References

[1]

"Complete sequence of Methanococcus aeolicus Nankai-3." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nankai-3 / ATCC BAA-1280.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000743 Genomic DNA. Translation: ABR56205.1.
RefSeq	YP_001324817.1. NC_009635.1.
3D structure databases
ProteinModelPortal	A6UUN3.
ModBase	Search...
Protein-protein interaction databases
STRING	A6UUN3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5327332.
GenomeReviews	Gene locus Maeo_0621 in contig CP000743_GR.
KEGG	mae:Maeo_0621.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04130.
HOGENOM	HBG392147.
OMA	MFTNSGM.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	MAEO419665:MAEO_0621-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_META3

Accession

Primary (citable) accession number: A6UUN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program