Reviewed, UniProtKB/Swiss-Prot A6UUD2 (G1PDH_META3)

Names and origin

Protein names

Recommended name:
    Glycerol-1-phosphate dehydrogenase [NAD(P)+]
      Short name=G1P dehydrogenase
      Short name=G1PDH
    EC=1.1.1.261
Alternative name(s):
    sn-glycerol-1-phosphate dehydrogenase
    Enantiomeric glycerophosphate synthase

Gene names

Name:	egsA
Ordered Locus Names:	Maeo_0518

Organism

Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) [Complete proteome] [HAMAP]

Taxonomic identifier

419665 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity.
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: InterPro glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497		PRO_0000350648
Regions
	Nucleotide binding	81 – 85	5	NAD By similarity
	Nucleotide binding	103 – 106	4	NAD By similarity
Sites
	Metal binding	151	1	Zinc; catalytic By similarity
	Metal binding	232	1	Zinc; catalytic By similarity
	Metal binding	253	1	Zinc; catalytic By similarity
	Binding site	108	1	Substrate By similarity
	Binding site	112	1	NAD By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	236	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6UUD2-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 4D67DC0BE5188E4A	FASTA	341	37,830
	10 20 30 40 50 60 MITTPRYIVI EENGINLLND ILLKLNLKNP LVITGKRTKK YISNFDYFYY FDYIDIKRNE 70 80 90 100 110 120 PNKEFIENIC NFDCIIGVGG GKAIDVGKYI AYKYNKQFIS IPTTASNDGI ASPIISQQGK 130 140 150 160 170 180 SITAESPIAI IADLNIIKKS PKRLLSAGMG DIVSNITAVL DWKLSYKETK EPYSESSAIF 190 200 210 220 230 240 SKTIAMELVE FVLSNDKNNL EEYPKKLVKA LIGSGITISI AGSTRPASGS EHLFSHSLDI 250 260 270 280 290 300 ITKKLNLNIN GIHGEQCGIG TIISAYLHLI EGNITIGEYE NIKLSLEKVG APTIGEQLGY 310 320 330 340 DKNILIDALA NAHKIRNRWT ILRNGISKEK AEKILKKTDI I

References

[1]

"Complete sequence of Methanococcus aeolicus Nankai-3." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000743 Genomic DNA. Translation: ABR56104.1.
RefSeq	YP_001324716.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6UUD2.
Genome annotation databases
GeneID	5327503.
GenomeReviews	Gene locus Maeo_0518 in contig CP000743_GR.
KEGG	mae:Maeo_0518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SESSAIF.
Family and domain databases
HAMAP	MF_00497. [Tree]
InterPro	IPR002658. DHQ_synth_AroB. [Graphical view]
Pfam	PF01761. DHQ_synthase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G1PDH_META3

Accession

Primary (citable) accession number: A6UUD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)