A6UTK4 (SYP_META3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Maeo_0234

Organism

Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) [Complete proteome] [HAMAP]

Taxonomic identifier

419665 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Proline--tRNA ligase HAMAP MF_01571		PRO_0000318775

Sequences

	Sequence		Length	Mass (Da)
	A6UTK4 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: A2489F6EFB42FF1D	FASTA	456	52,781
	10 20 30 40 50 60 MENFSEWYHN ILETAGIYDL RYPLKGCGVY LPYGFKIRRY AFEAIRDMLD ESNHDEALFP 70 80 90 100 110 120 MLIPEDLLAK EGEHIKGFEE EVYWVTHGGT TPLDVKLALR PTSETPIYHM MKLWIKVHTD 130 140 150 160 170 180 LPIKIYQIVN SFRYETKHTR PLIRLREIMT FKEAHTAHST SEEAEAQVQT ALNIYKTFFD 190 200 210 220 230 240 RMGVPTIVSQ RPEWDKFPGA DYTMAFDTIF PDGKTMQIGT VHNLGQHFAK TFELEFETPE 250 260 270 280 290 300 GGKDYAYQTC YGISDRIIAS IIALHGDEKG LILPPEVAPH QIIIIPLLFK GKEEIAMNKA 310 320 330 340 350 360 KEIYKSLKNT YRVKLDDRDI RPGKKFNDWE LKGAPIRIEL GPRDIENNKL TIYRRDTGEK 370 380 390 400 410 420 FQIDEDNLLN ELNTLIDSIE NTIKEKAEQK VKSFITILDN HDVNNIKETL STKKGVVLVP 430 440 450 YDENIYTEEF EEEIDASVLG TTEYDGKKYI SIAKTY

References

[1]

"Complete sequence of Methanococcus aeolicus Nankai-3." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nankai-3 / ATCC BAA-1280.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000743 Genomic DNA. Translation: ABR55826.1.
RefSeq	YP_001324438.1. NC_009635.1.
3D structure databases
ProteinModelPortal	A6UTK4.
SMR	A6UTK4. Positions 1-456.
ModBase	Search...
Protein-protein interaction databases
STRING	A6UTK4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5326817.
GenomeReviews	Gene locus Maeo_0234 in contig CP000743_GR.
KEGG	mae:Maeo_0234.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	MAEO419665:MAEO_0234-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR015264. Pro-tRNA_synth_II_arc. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09181. ProRS-C_2. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_META3

Accession

Primary (citable) accession number: A6UTK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program