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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13SubstrateUniRule annotation1
Binding sitei69SubstrateUniRule annotation1
Active sitei78Proton donorUniRule annotation1
Binding sitei173SubstrateUniRule annotation1
Sitei175Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei212SubstrateUniRule annotation1
Sitei230Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei239Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciMAEO419665:G1G9A-205-MONOMER
UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:Maeo_0204
OrganismiMethanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)
Taxonomic identifieri419665 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000001106 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000119031 – 295Diaminopimelate epimeraseAdd BLAST295

Proteomic databases

PRIDEiA6UTH4

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi419665.Maeo_0204

Structurei

3D structure databases

ProteinModelPortaliA6UTH4
SMRiA6UTH4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 80Substrate bindingUniRule annotation2
Regioni230 – 231Substrate bindingUniRule annotation2
Regioni240 – 241Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02255 Archaea
COG0253 LUCA
HOGENOMiHOG000220466
KOiK01778
OMAiSMCGNGG
OrthoDBiPOG093Z085A

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

A6UTH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLNFTKMHG LGNDYIVINE FDGEKIKEDE KAEFSKKICK RGFSIGADGV
60 70 80 90 100
IFVQPATDEK YDIRFRIFNS DGSEAEMCGN GIRCFSKFVY ERVMQKNPLN
110 120 130 140 150
VETMGGLRVS EMNIENNIVK SIKVFMGAPT FELKDIPMVV EGKSENDVFL
160 170 180 190 200
DEEIELKNAL LNSVKLSVVN VGNPHAVIFM KDNNLKAPFI RTHLNILGKE
210 220 230 240 250
IEHHSVFPEK INVHFVEIVN PQEIKIITWE RGAGYTTACG TGTTSSVIIA
260 270 280 290
NKLGKTGNKV LAHLDGGDLE IEIKEDGVYM EGDAVIVYDG ELYQY
Length:295
Mass (Da):32,981
Last modified:August 21, 2007 - v1
Checksum:i5547FADE4D5EF07D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000743 Genomic DNA Translation: ABR55796.1
RefSeqiWP_011972928.1, NC_009635.1

Genome annotation databases

EnsemblBacteriaiABR55796; ABR55796; Maeo_0204
GeneIDi5326820
KEGGimae:Maeo_0204

Similar proteinsi

Entry informationi

Entry nameiDAPF_META3
AccessioniPrimary (citable) accession number: A6UTH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: May 23, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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