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A6UTF3 (BIOB_META3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Maeo_0183
OrganismMethanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) [Complete proteome] [HAMAP]
Taxonomic identifier419665 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325Biotin synthase HAMAP-Rule MF_01694
PRO_0000381457

Sites

Metal binding641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1071Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1391Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1981Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2741Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A6UTF3 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: A77D4F84BC544EC5

FASTA32536,834
        10         20         30         40         50         60 
MEEFTGIGYF YKKSIKNQLD KKDASLLWEL PYYTLLYLAN CVKKHNNSNN IDLCSIINAK 

        70         80         90        100        110        120 
SGLCSENCAF CSQSLHNSSK IKEYPLKPKE DILKQAKYIE KYSNRFSIVV SGKTVNDREY 

       130        140        150        160        170        180 
NEIIDAIKDI KKETNLKVCA SLGLLDNSQL KELKDLDVRI HNNLETSKEY FDKICTTHTY 

       190        200        210        220        230        240 
DDKVKSITMA KKIGLEVCSG GIIGLGESLE NRINLFYELK ELDVEGIPIN IYNPIKGTKT 

       250        260        270        280        290        300 
YELLNNNAIK QITPIEALKS IAICKLILPN KDVRLCGGRE YNLRDWQSLS LLAIDGLMIG 

       310        320 
NYLTTNGRNI EDDIKMIVDG GFDGK 

« Hide

References

[1]"Complete sequence of Methanococcus aeolicus Nankai-3."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. expand/collapse author list , Sieprawska-Lupa M., Whitman W.B., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nankai-3 / ATCC BAA-1280.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000743 Genomic DNA. Translation: ABR55775.1.
RefSeqYP_001324387.1. NC_009635.1.

3D structure databases

ProteinModelPortalA6UTF3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING419665.Maeo_0183.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR55775; ABR55775; Maeo_0183.
GeneID5326588.
KEGGmae:Maeo_0183.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.

Enzyme and pathway databases

BioCycMAEO419665:GHVZ-184-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_META3
AccessionPrimary (citable) accession number: A6UTF3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways