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A6UTF3

- BIOB_META3

UniProt

A6UTF3 - BIOB_META3

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Protein

Biotin synthase

Gene

bioB

Organism
Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi107 – 1071Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi139 – 1391Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi274 – 2741Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMAEO419665:GHVZ-184-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Maeo_0183
OrganismiMethanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280)
Taxonomic identifieri419665 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000001106: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Biotin synthasePRO_0000381457Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi419665.Maeo_0183.

Structurei

3D structure databases

ProteinModelPortaliA6UTF3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiCIARITL.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A6UTF3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEFTGIGYF YKKSIKNQLD KKDASLLWEL PYYTLLYLAN CVKKHNNSNN
60 70 80 90 100
IDLCSIINAK SGLCSENCAF CSQSLHNSSK IKEYPLKPKE DILKQAKYIE
110 120 130 140 150
KYSNRFSIVV SGKTVNDREY NEIIDAIKDI KKETNLKVCA SLGLLDNSQL
160 170 180 190 200
KELKDLDVRI HNNLETSKEY FDKICTTHTY DDKVKSITMA KKIGLEVCSG
210 220 230 240 250
GIIGLGESLE NRINLFYELK ELDVEGIPIN IYNPIKGTKT YELLNNNAIK
260 270 280 290 300
QITPIEALKS IAICKLILPN KDVRLCGGRE YNLRDWQSLS LLAIDGLMIG
310 320
NYLTTNGRNI EDDIKMIVDG GFDGK
Length:325
Mass (Da):36,834
Last modified:August 21, 2007 - v1
Checksum:iA77D4F84BC544EC5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000743 Genomic DNA. Translation: ABR55775.1.
RefSeqiWP_011972907.1. NC_009635.1.
YP_001324387.1. NC_009635.1.

Genome annotation databases

EnsemblBacteriaiABR55775; ABR55775; Maeo_0183.
GeneIDi5326588.
KEGGimae:Maeo_0183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000743 Genomic DNA. Translation: ABR55775.1 .
RefSeqi WP_011972907.1. NC_009635.1.
YP_001324387.1. NC_009635.1.

3D structure databases

ProteinModelPortali A6UTF3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 419665.Maeo_0183.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR55775 ; ABR55775 ; Maeo_0183 .
GeneIDi 5326588.
KEGGi mae:Maeo_0183.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi CIARITL.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MAEO419665:GHVZ-184-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nankai-3 / ATCC BAA-1280.

Entry informationi

Entry nameiBIOB_META3
AccessioniPrimary (citable) accession number: A6UTF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3