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A6UTF3

- BIOB_META3

UniProt

A6UTF3 - BIOB_META3

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Protein
Biotin synthase
Gene
bioB, Maeo_0183
Organism
Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi107 – 1071Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi139 – 1391Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi274 – 2741Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMAEO419665:GHVZ-184-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Maeo_0183
OrganismiMethanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280)
Taxonomic identifieri419665 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000001106: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Biotin synthaseUniRule annotation
PRO_0000381457Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi419665.Maeo_0183.

Structurei

3D structure databases

ProteinModelPortaliA6UTF3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiCIARITL.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A6UTF3-1 [UniParc]FASTAAdd to Basket

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MEEFTGIGYF YKKSIKNQLD KKDASLLWEL PYYTLLYLAN CVKKHNNSNN    50
IDLCSIINAK SGLCSENCAF CSQSLHNSSK IKEYPLKPKE DILKQAKYIE 100
KYSNRFSIVV SGKTVNDREY NEIIDAIKDI KKETNLKVCA SLGLLDNSQL 150
KELKDLDVRI HNNLETSKEY FDKICTTHTY DDKVKSITMA KKIGLEVCSG 200
GIIGLGESLE NRINLFYELK ELDVEGIPIN IYNPIKGTKT YELLNNNAIK 250
QITPIEALKS IAICKLILPN KDVRLCGGRE YNLRDWQSLS LLAIDGLMIG 300
NYLTTNGRNI EDDIKMIVDG GFDGK 325
Length:325
Mass (Da):36,834
Last modified:August 21, 2007 - v1
Checksum:iA77D4F84BC544EC5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000743 Genomic DNA. Translation: ABR55775.1.
RefSeqiYP_001324387.1. NC_009635.1.

Genome annotation databases

EnsemblBacteriaiABR55775; ABR55775; Maeo_0183.
GeneIDi5326588.
KEGGimae:Maeo_0183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000743 Genomic DNA. Translation: ABR55775.1 .
RefSeqi YP_001324387.1. NC_009635.1.

3D structure databases

ProteinModelPortali A6UTF3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 419665.Maeo_0183.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR55775 ; ABR55775 ; Maeo_0183 .
GeneIDi 5326588.
KEGGi mae:Maeo_0183.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi CIARITL.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MAEO419665:GHVZ-184-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nankai-3 / ATCC BAA-1280.

Entry informationi

Entry nameiBIOB_META3
AccessioniPrimary (citable) accession number: A6UTF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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