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A6UT22

- HEM1_META3

UniProt

A6UT22 - HEM1_META3

Protein

Glutamyl-tRNA reductase

Gene

hemA

Organism
Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).UniRule annotation

    Catalytic activityi

    L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei39 – 391NucleophileUniRule annotation
    Sitei72 – 721Important for activityUniRule annotation
    Binding sitei82 – 821SubstrateUniRule annotation
    Binding sitei93 – 931SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi161 – 1666NADPUniRule annotation

    GO - Molecular functioni

    1. glutamyl-tRNA reductase activity Source: UniProtKB-HAMAP
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Porphyrin biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMAEO419665:GHVZ-52-MONOMER.
    UniPathwayiUPA00251; UER00316.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamyl-tRNA reductaseUniRule annotation (EC:1.2.1.70UniRule annotation)
    Short name:
    GluTRUniRule annotation
    Gene namesi
    Name:hemAUniRule annotation
    Ordered Locus Names:Maeo_0052
    OrganismiMethanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280)
    Taxonomic identifieri419665 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
    ProteomesiUP000001106: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 383383Glutamyl-tRNA reductasePRO_1000004635Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi419665.Maeo_0052.

    Structurei

    3D structure databases

    ProteinModelPortaliA6UT22.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni38 – 414Substrate bindingUniRule annotation
    Regioni87 – 893Substrate bindingUniRule annotation

    Domaini

    Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.UniRule annotation

    Sequence similaritiesi

    Belongs to the glutamyl-tRNA reductase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0373.
    HOGENOMiHOG000109651.
    KOiK02492.
    OMAiEHMIEDE.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00087. Glu_tRNA_reductase.
    InterProiIPR000343. 4pyrrol_synth_GluRdtase.
    IPR015896. 4pyrrol_synth_GluRdtase_dimer.
    IPR015895. 4pyrrol_synth_GluRdtase_N.
    IPR016040. NAD(P)-bd_dom.
    IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
    [Graphical view]
    PfamiPF00745. GlutR_dimer. 1 hit.
    PF05201. GlutR_N. 1 hit.
    PF01488. Shikimate_DH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
    SUPFAMiSSF69075. SSF69075. 1 hit.
    SSF69742. SSF69742. 1 hit.
    TIGRFAMsiTIGR01035. hemA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A6UT22-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLFRADYNN YAVSELQKLR FDEDEFYKKY DNCVLVQTCN RIEIYFDKNA    50
    KIIDINEFAE FEMIKSNNAI KHLLRTASGL NSMIVGEDQI IGQIKNSHRK 100
    AKELKKTTKY LDTIFLKAIH TGQKVRNNTK INKGCVSIGS AAVQLAEKTV 150
    GLNNKNILVV GAGEIATLVA KALIEKNIRA IVVSNRTYER AELLAKKLNG 200
    MAVHFDKLGE AINYNDIIIC ATGAPHAIID KDRLKNIKGY KVLIDIANPR 250
    DVSDDVMELP NIKLYTIDDL KMVSEENLKK RKDEIPRVEK IIEEELSVLT 300
    KQLRKLKFEN TIKNYDLYIE NLRKREMNKA LNMIENGKDP TVVLEKFSKV 350
    FANKLISDFV NIVNDDTIGD IERVVNKLNK NKI 383
    Length:383
    Mass (Da):43,752
    Last modified:August 21, 2007 - v1
    Checksum:i24EA373469361A60
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000743 Genomic DNA. Translation: ABR55644.1.
    RefSeqiWP_011972776.1. NC_009635.1.
    YP_001324256.1. NC_009635.1.

    Genome annotation databases

    EnsemblBacteriaiABR55644; ABR55644; Maeo_0052.
    GeneIDi5326829.
    KEGGimae:Maeo_0052.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000743 Genomic DNA. Translation: ABR55644.1 .
    RefSeqi WP_011972776.1. NC_009635.1.
    YP_001324256.1. NC_009635.1.

    3D structure databases

    ProteinModelPortali A6UT22.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 419665.Maeo_0052.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR55644 ; ABR55644 ; Maeo_0052 .
    GeneIDi 5326829.
    KEGGi mae:Maeo_0052.

    Phylogenomic databases

    eggNOGi COG0373.
    HOGENOMi HOG000109651.
    KOi K02492.
    OMAi EHMIEDE.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00316 .
    BioCyci MAEO419665:GHVZ-52-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00087. Glu_tRNA_reductase.
    InterProi IPR000343. 4pyrrol_synth_GluRdtase.
    IPR015896. 4pyrrol_synth_GluRdtase_dimer.
    IPR015895. 4pyrrol_synth_GluRdtase_N.
    IPR016040. NAD(P)-bd_dom.
    IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
    [Graphical view ]
    Pfami PF00745. GlutR_dimer. 1 hit.
    PF05201. GlutR_N. 1 hit.
    PF01488. Shikimate_DH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
    SUPFAMi SSF69075. SSF69075. 1 hit.
    SSF69742. SSF69742. 1 hit.
    TIGRFAMsi TIGR01035. hemA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Nankai-3 / ATCC BAA-1280.

    Entry informationi

    Entry nameiHEM1_META3
    AccessioniPrimary (citable) accession number: A6UT22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3