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A6UT04 (PURA_META3) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenylosuccinate synthetase
Short name=AMPSase
Short name=AdSS
EC=6.3.4.4
Alternative name(s):
IMP--aspartate ligase
Gene names
Name:purA
Ordered Locus Names:Maeo_0033
OrganismMethanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) [Complete proteome] [HAMAP]
Taxonomic identifier419665 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011

Catalytic activity

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011

Subunit structure

Homodimer By similarity. HAMAP MF_00011

Subcellular location

Cytoplasm By similarity HAMAP MF_00011.

Sequence similarities

Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processpurine nucleotide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

adenylosuccinate synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Adenylosuccinate synthetase HAMAP MF_00011
PRO_0000321816

Regions

Nucleotide binding12 – 187GTP By similarity
Nucleotide binding42 – 443GTP By similarity
Nucleotide binding284 – 2863GTP By similarity
Nucleotide binding324 – 3263GTP By similarity
Region13 – 164IMP binding By similarity
Region40 – 434IMP binding By similarity
Region252 – 2587Substrate binding By similarity

Sites

Active site131Proton acceptor By similarity
Active site431Proton donor By similarity
Metal binding131Magnesium By similarity
Metal binding421Magnesium; via carbonyl oxygen By similarity
Binding site1271IMP By similarity
Binding site1411IMP; shared with dimeric partner By similarity
Binding site1791IMP By similarity
Binding site1941IMP By similarity
Binding site2561IMP By similarity
Binding site2581GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6UT04 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 5D690B86A0D00694

FASTA33636,542
        10         20         30         40         50         60 
MTCTIVVGGQ WGDEGKGKII SYICEKDKPS VVARGGVGPN AGHSVEVDGK KYGIRMVPTG 

        70         80         90        100        110        120 
FPNRSSKLAV GAGVLVDVDV LLKEVEMLKE FNIKDRVIID YNCGVIEEKH KEMDKSNSHL 

       130        140        150        160        170        180 
SKEIGSTGTG CGPANVDRAM RTLRLAKEFD ALKPFLGDVS DLVNEALENG ENVLIEGTQG 

       190        200        210        220        230        240 
TLLSLYYGSY PYVTSNDTSA SAFASDVGIG PTKVDEVIVV FKSYPTRVGE GPFPTEMDVE 

       250        260        270        280        290        300 
EAEKLGLVEY GTVTGRRRRI GYFDYDLAKK VCRLNGATAI ALTCLDKYDE ECHGLTNYDD 

       310        320        330 
ITEKGLAFIK EIEEKVGVPV KIISTGPELH QTIDIR

« Hide

References

[1]"Complete sequence of Methanococcus aeolicus Nankai-3."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. expand/collapse author list , Sieprawska-Lupa M., Whitman W.B., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nankai-3 / ATCC BAA-1280.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000743 Genomic DNA. Translation: ABR55626.1.
RefSeqYP_001324238.1. NC_009635.1.

3D structure databases

ProteinModelPortalA6UT04.
SMRA6UT04. Positions 1-336.
ModBaseSearch...

Protein-protein interaction databases

STRINGA6UT04.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5326450.
GenomeReviewsGene locus Maeo_0033 in contig CP000743_GR.
KEGGmae:Maeo_0033.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG04146.
HOGENOMHBG658237.
OMAYVLGIIK.
ProtClustDBPRK04293.

Enzyme and pathway databases

BioCycMAEO419665:MAEO_0033-MONOMER.

Family and domain databases

HAMAPMF_00011. Adenylosucc_synth.
[Tree]
InterProIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
[Graphical view]
KOK01939.
PANTHERPTHR11846. Asucc_synthtase. 1 hit.
PfamPF00709. Adenylsucc_synt. 2 hits.
[Graphical view]
SMARTSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
PROSITEPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePURA_META3
AccessionPrimary (citable) accession number: A6UT04
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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