A6US09 (SYA_METVS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Mevan_1384

Organism

Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) [Complete proteome] [HAMAP]

Taxonomic identifier

406327 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	892 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 892	892	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_1000074513
Sites
	Metal binding	596	1	Zinc By similarity
	Metal binding	600	1	Zinc By similarity
	Metal binding	700	1	Zinc By similarity
	Metal binding	704	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6US09 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 12FC466D1C26F59E	FASTA	892	101,699
	10 20 30 40 50 60 MEITHDYKVK LFEEMNFERK QCSECKQWFW TLDKERITCG DSPCDKYSFI GNPITTKKYT 70 80 90 100 110 120 YNEMVKEFTN FFDERGHSPV KRSPVIAKRW RDDILLTIAS IAVFQPWVTN GLVKPVKNPL 130 140 150 160 170 180 VITQPCIRLN DIDNVGRTGR HLTCFTMGAH HAFNSKDEYK YWTDKTVELC FELMQKLGID 190 200 210 220 230 240 GHTITFIESW WEGGGNAGPC YEVITHGVEL ATLVFMQYKK VGNNYEEIPL KIVDTGYGIE 250 260 270 280 290 300 RFAWASQGTP TVYEALFSDI IEKLKKNANI PIVDEKIMAE SATLAGLMDI ENVGDLKALR 310 320 330 340 350 360 QKVAEKIGMD VNELDRLISP LEYIYAISDH TRCLSFMFGD GIVPSNVKEG YLARLVLRKT 370 380 390 400 410 420 LRYMEKVGIS YSIKEIILMQ LESMKEVYPE LIGMKDYIMD VLDSEEKKYI QTVNKGRGIV 430 440 450 460 470 480 ERMTKLKSEI TLNDLINLYD SNGLPPEIVK DIIDEINKTS KTKISIHVPD NFYTIVAERH 490 500 510 520 530 540 EEEKAEEVAI LKHELPELDL PKTELLFFKN TMQSEFEAKV LKIVDKYIIL DRTVFYAEGG 550 560 570 580 590 600 GQKYDIGQIS GVDVVDVQKK NGIVFHKVLD ISKFKEGNLV KGELNFENRL KLMRNHTATH 610 620 630 640 650 660 VINAALKQVL GKHVWQTGSN VDTEKGRLDV THYERISRKE IKEIEKIANE IVLLGKPVTC 670 680 690 700 710 720 KFMDRNDAEQ EYGFKIYQGG VVPGDTLRIV EIDGIDVEAC GGTHVTNTSE IGYIKVLKTE 730 740 750 760 770 780 RIQDGVERLE YSTGMGSIFE IAALEDILLD SAEVLGVPIE NLPKTAKRFF EEWKEQKKVI 790 800 810 820 830 840 EELQKKVGEL LKYELLEKFE KVGNLEILVE KVSGTSNELM AIADNLATNG KIVILMNDTD 850 860 870 880 890 YILCKKGENV EISMKELIQK IGKGGGKENL AQGKYSTSKE QIREKAFELL KQ

References

[1]

"Complete sequence of Methanococcus vannielii SB." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SB / ATCC 35089 / DSM 1224.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000742 Genomic DNA. Translation: ABR55281.1.
RefSeq	YP_001323893.1. NC_009634.1.
3D structure databases
ProteinModelPortal	A6US09.
ModBase	Search...
Protein-protein interaction databases
STRING	A6US09.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5324766.
GenomeReviews	Gene locus Mevan_1384 in contig CP000742_GR.
KEGG	mvn:Mevan_1384.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04130.
HOGENOM	HBG392147.
OMA	MFTNSGM.
ProtClustDB	PRK13902.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_METVS

Accession

Primary (citable) accession number: A6US09

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program