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A6US04 (PUR2_METVS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Mevan_1379
OrganismMethanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) [Complete proteome] [HAMAP]
Taxonomic identifier406327 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000018830

Regions

Domain109 – 324216ATP-grasp
Nucleotide binding140 – 20263ATP By similarity

Sites

Metal binding2821Magnesium or manganese 1 By similarity
Metal binding2941Magnesium or manganese 1 By similarity
Metal binding2941Magnesium or manganese 2 By similarity
Metal binding2961Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A6US04 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 766525AA8170DE24

FASTA44348,771
        10         20         30         40         50         60 
MKVLLIGGGA REHAIAMALK KNRSVELYTL MKNKNPGIYG LSKEVSFNNE TDIDKIKEFA 

        70         80         90        100        110        120 
EKIRPELAII GPEAPLGVGA ADLLTEMGIP TVGPKKLPAQ IETSKEFMRN LFKKYNIKGS 

       130        140        150        160        170        180 
LKYAAFNEYG ENLEKFIDEM TGLGKDVVVK PAGLTGGKGV KVVGEQLKNN DDAKLYAKEV 

       190        200        210        220        230        240 
FEKSIGGGKI IVEEKLVGVE FTLHGFVDGE NIVFMPAVQD HPHAYNNDEG PITGGMGSYS 

       250        260        270        280        290        300 
CSNHKLPFLP DDKLDEAKEI MKETVNAIKA EVGPYNGFLY GQFMLTKDGP KIIEYNARFG 

       310        320        330        340        350        360 
DPEAMNLLPI LKTDFLDVCF GISNGNLDNI NIEFENKATV CKYVVPNGYP ICPVKNKEIL 

       370        380        390        400        410        420 
VNSNEIEKAG AILFYASVNE ENGKLVITGS RSAAVTGISE RIEDAEKIAQ DAIINFEGEI 

       430        440 
FFRSDIGTKK LIEKRIERMK ELI 

« Hide

References

[1]"Complete sequence of Methanococcus vannielii SB."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I. expand/collapse author list , Sieprawska-Lupa M., Whitman W.B., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SB / ATCC 35089 / DSM 1224.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000742 Genomic DNA. Translation: ABR55276.1.
RefSeqYP_001323888.1. NC_009634.1.

3D structure databases

ProteinModelPortalA6US04.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING406327.Mevan_1379.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR55276; ABR55276; Mevan_1379.
GeneID5324746.
KEGGmvn:Mevan_1379.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMADPEAMNV.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycMVAN406327:GI04-1428-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METVS
AccessionPrimary (citable) accession number: A6US04
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways