A6URW1 (G3P_METVS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase
Short name=GAPDH
EC=1.2.1.59

Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	Mevan_1336

Organism

Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) [Complete proteome] [HAMAP]

Taxonomic identifier

406327 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity. HAMAP MF_00559
Subcellular location	Cytoplasm By similarity HAMAP MF_00559.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_1000061119
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	140 – 142	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	195 – 196	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	141	1	Nucleophile By similarity
	Binding site	111	1	NAD; via amide nitrogen By similarity
	Binding site	169	1	NAD By similarity
	Binding site	303	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6URW1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: DD6E8BC987A1DA1D	FASTA	340	37,553
	10 20 30 40 50 60 MVGVLVNGYG SIGKRVADAV AKQDDMKVIG VTKTKPDFEA RMAVTKGYKL FAAIPEKKHL 70 80 90 100 110 120 FEEANIPIEG TIEDIIEDAD IVVDGAPKKI GKANVENVYK KHNVKAIIQG GEKAKDAEDS 130 140 150 160 170 180 FNSLWSYNRC YGKDYIRLVS CNTTGLCRTL YAIDSITDIL KARVVLVRRA ADPNDIKTGP 190 200 210 220 230 240 INAIIPDPVT VPSHHGPDVV SVIPKLDGKI MTSAIIVPTT LMHMHSLMVE TTGVTKDAVL 250 260 270 280 290 300 DAIEKTPRII KVKASEGIDS TAKIIEYSRD LGRLRYDLNE IAIWEESINV VDNEIYLMQA 310 320 330 340 IHQESDVIPE NIDCIRAMLE MEEDNIKSIE KTNRALGLLK

References

[1]

"Complete sequence of Methanococcus vannielii SB." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SB / ATCC 35089 / DSM 1224.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000742 Genomic DNA. Translation: ABR55233.1.
RefSeq	YP_001323845.1. NC_009634.1.
3D structure databases
ProteinModelPortal	A6URW1.
SMR	A6URW1. Positions 4-338.
ModBase	Search...
Protein-protein interaction databases
STRING	A6URW1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5324771.
GenomeReviews	Gene locus Mevan_1336 in contig CP000742_GR.
KEGG	mvn:Mevan_1336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04694.
HOGENOM	HBG392099.
OMA	VPSHHGP.
ProtClustDB	PRK04207.
Family and domain databases
HAMAP	MF_00559. G3P_dehdrog_arch. [Tree]
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00150.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_METVS

Accession

Primary (citable) accession number: A6URW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program