Reviewed, UniProtKB/Swiss-Prot A6URH2 (DP2L_METVS)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	Mevan_1196

Organism

Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) [Complete proteome] [HAMAP]

Taxonomic identifier

406327 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	1131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1131	1131	DNA polymerase II large subunit		PRO_1000019391

Sequences

	Sequence		Length	Mass (Da)
	A6URH2-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 82E2CC6A4100753E	FASTA	1,131	128,607
	10 20 30 40 50 60 MLHVSASKNM TEYFKSILND VNNLYFIAEE CRKLGYDVVD KVEIPLAKDM ADRVEGIVGP 70 80 90 100 110 120 PKVSERIREL VMELGKEPAA LEIAKEIVEG RFGEFGKEEG AEQAVRTALA VITEGIVAAP 130 140 150 160 170 180 LEGIAHVKIK KNHDGTEYLA IYFAGPIRSA GGTAQALAVL VGDYVRKNMN LDKFKPTDDE 190 200 210 220 230 240 VERYGEEIDL YQSEVTTFQY QPKIEEIRTA VRNISVEITG EATDDVEVSG HRDLERVETN 250 260 270 280 290 300 QIRGGALLAL VEGVLLKAPK ILRHVDKLEI EGWNWLKELK NKKEEINEDI KDEKEDFNYE 310 320 330 340 350 360 EEEDLSQYDD CEIEEVTKFI GEVIAGRPVF AHPSKKGGFR LRYGRSRNTG FATDGFHPAI 370 380 390 400 410 420 MYLVDDFMAV GTQLKTERPG KATCVVPVDS IDPPIVKLNN HSVLKIDTVE KAIKYRKDVL 430 440 450 460 470 480 EILFLGDILV NYGDFLENNH VMLPSSWCVE WYEKILKVNN IPYSTEFISN PSPKEAVKFA 490 500 510 520 530 540 ITTKTPLHPV YTYHWHDISK ENIYSLRNWI LRGNFNKNSD KWEISYDLEN PEDISNKRFL 550 560 570 580 590 600 ELIGCCHEVV DGKIFILEYY PLLYSLGYDY ENSFDSVENL EEKVSNAKNN MHLINLLSHF 610 620 630 640 650 660 EIRRNTYIYV GARMGRPEKA ASRKMKPPVN GLFPIGNAGA LVRLINKAVE GGKTDEIEIS 670 680 690 700 710 720 NVMCSCGKVS LYKKCPFCGK SVIPTGPTRI KLPIKDYWYN ALENLKINKP GDVKCIKGMT 730 740 750 760 770 780 SKDKIIEPLE KAILRAVNDV YVFKDGTTRF DCTDVPVTHF KPCEINVSVN RLKELGYLRD 790 800 810 820 830 840 INGNSLENDK QVLELKVQDV IVPESCMDYF LNVSKFIDDL LEKYYKKNRY YNSSNKEDLV 850 860 870 880 890 900 GHLIIGMAPH TSAGMVGRIV GYSKANVGYA HPYFHASKRR NCDGDEDAFF LLLDAFLNFS 910 920 930 940 950 960 KKFLPDKRGG QMDAPLVLTT ILDPKEVDGE VHNMDSMWEY PLEFYEKSLE MVTPKEIKKL 970 980 990 1000 1010 1020 METVEDRLGK DEQYEGIGYT HETLKIDEGP LICSYKTLGS MMDKTSAQLA VAKKIRATDE 1030 1040 1050 1060 1070 1080 RDVAEKVIQS HFVPDLIGNL RAFSRQGVRC KCGAKYRRIP LKGVCRKCGS RLILTVSKGA 1090 1100 1110 1120 1130 VEKYMNVSQT MAEKYNVSDY IKQRLEIIRS GIDSLFTNDK RKQVKIEDFF K

References

[1]

"Complete sequence of Methanococcus vannielii SB." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000742 Genomic DNA. Translation: ABR55094.1.
RefSeq	YP_001323706.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5325524.
GenomeReviews	Gene locus Mevan_1196 in contig CP000742_GR.
KEGG	mvn:Mevan_1196.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METVS

Accession

Primary (citable) accession number: A6URH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	July 22, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)