A6URG2 (RIFK_METVS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	Mevan_1186

Organism

Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) [Complete proteome] [HAMAP]

Taxonomic identifier

406327 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 130	130	Riboflavin kinase HAMAP MF_01285		PRO_0000322074
Regions
	Nucleotide binding	10 – 15	6	CDP By similarity
	Nucleotide binding	109 – 112	4	CDP By similarity
Sites
	Metal binding	39	1	Magnesium By similarity
	Metal binding	41	1	Magnesium By similarity
	Binding site	96	1	FMN By similarity
	Binding site	104	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6URG2 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: C7FF82C7DD6E5DEF	FASTA	130	14,781
	10 20 30 40 50 60 MKIFGRVVSG FGEGKYFVGL IPYKNKFKEL TGFTPYEGTL NIKLKTYFDI DKYDPLEFDG 70 80 90 100 110 120 FEIDGKEYFG GKVLLVTLFN KSGKFVDCAI VSPKKTDHSK KTLEIIAPVN LRKFLSLKNL 130 DIVKIIQALK

References

[1]

"Complete sequence of Methanococcus vannielii SB." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SB / ATCC 35089 / DSM 1224.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000742 Genomic DNA. Translation: ABR55084.1.
RefSeq	YP_001323696.1. NC_009634.1.
3D structure databases
ProteinModelPortal	A6URG2.
SMR	A6URG2. Positions 1-129.
ModBase	Search...
Protein-protein interaction databases
STRING	A6URG2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5325344.
GenomeReviews	Gene locus Mevan_1186 in contig CP000742_GR.
KEGG	mvn:Mevan_1186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG11680.
HOGENOM	HBG553349.
OMA	PYEGTLN.
ProtClustDB	PRK14132.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_METVS

Accession

Primary (citable) accession number: A6URG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program