Reviewed, UniProtKB/Swiss-Prot A6URG2 (RIFK_METVS)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	Mevan_1186

Organism

Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) [Complete proteome] [HAMAP]

Taxonomic identifier

406327 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 130	130	Riboflavin kinase HAMAP MF_01285		PRO_0000322074
Regions
	Nucleotide binding	10 – 15	6	CDP By similarity
	Nucleotide binding	109 – 112	4	CDP By similarity
Sites
	Metal binding	39	1	Magnesium By similarity
	Metal binding	41	1	Magnesium By similarity
	Binding site	96	1	FMN By similarity
	Binding site	104	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6URG2-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: C7FF82C7DD6E5DEF	FASTA	130	14,781
	10 20 30 40 50 60 MKIFGRVVSG FGEGKYFVGL IPYKNKFKEL TGFTPYEGTL NIKLKTYFDI DKYDPLEFDG 70 80 90 100 110 120 FEIDGKEYFG GKVLLVTLFN KSGKFVDCAI VSPKKTDHSK KTLEIIAPVN LRKFLSLKNL 130 DIVKIIQALK

References

[1]

"Complete sequence of Methanococcus vannielii SB." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000742 Genomic DNA. Translation: ABR55084.1.
RefSeq	YP_001323696.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6URG2.
Genome annotation databases
GeneID	5325344.
GenomeReviews	Gene locus Mevan_1186 in contig CP000742_GR.
KEGG	mvn:Mevan_1186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KTDHSKK.
Family and domain databases
HAMAP	MF_01285. [Tree]
InterPro	IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProDom	PD015839. DUF120. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RIFK_METVS

Accession

Primary (citable) accession number: A6URG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)