A6URA0 (COFC_METVS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-phospho-L-lactate guanylyltransferase
Short name=LP guanylyltransferase
EC=2.7.7.68

Gene names

Name:	cofC
Ordered Locus Names:	Mevan_1122

Organism

Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) [Complete proteome] [HAMAP]

Taxonomic identifier

406327 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus › Methanococcus vannielii

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Guanylyltransferase that catalyzes the activation of 2-phospho-L-lactate (LP) as (2S)-lactyl-2-diphospho-5'-guanosine (LPPG), via the condensation of LP with GTP. Is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor By similarity. HAMAP-Rule MF_02114
Catalytic activity	(2S)-2-phospholactate + GTP = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate. HAMAP-Rule MF_02114
Pathway	Cofactor biosynthesis; coenzyme F420 biosynthesis. HAMAP-Rule MF_02114
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the CofC family.

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	coenzyme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW phospholactate guanylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	2-phospho-L-lactate guanylyltransferase HAMAP-Rule MF_02114		PRO_0000398748

Sequences

	Sequence		Length	Mass (Da)
	A6URA0 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 327C69B89D7C0FBA	FASTA	224	25,209
	10 20 30 40 50 60 MLAALIPVSP LSNVKSRLKE FLSSKERIDL IKNILLDTYE KVKSCVDACY VVSKDDEILE 70 80 90 100 110 120 FSKNLGIIPI KEDLNTKGLN EAIEYSLNII KEDSVLITPA DVPLLKEENL ESIVKKSVEN 130 140 150 160 170 180 SVIICPSRGG GTNLLLLNPK NCIKPQFEGF SFLKHIKEAE INNLNIIKCH SFYTSIDINT 190 200 210 220 VEDLGEIFIH GKDTKTYKFL KNLNIEAFPK HSSAGRFNII RKSQ

References

[1]

"Complete sequence of Methanococcus vannielii SB." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SB / ATCC 35089 / DSM 1224.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000742 Genomic DNA. Translation: ABR55022.1.
RefSeq	YP_001323634.1. NC_009634.1.
3D structure databases
ProteinModelPortal	A6URA0.
ModBase	Search...
Protein-protein interaction databases
STRING	406327.Mevan_1122.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABR55022; ABR55022; Mevan_1122.
GeneID	5326069.
KEGG	mvn:Mevan_1122.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1920.
HOGENOM	HOG000223639.
KO	K14941.
OMA	LGEIFIH.
ProtClustDB	CLSK876373.
Enzyme and pathway databases
BioCyc	MVAN406327:GI04-1173-MONOMER.
UniPathway	UPA00071.
Family and domain databases
HAMAP	MF_02114. CofC.
InterPro	IPR002835. Coenzyme_F420_CofC. [Graphical view]
Pfam	PF01983. CofC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03552. F420_cofC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COFC_METVS

Accession

Primary (citable) accession number: A6URA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program