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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei55 – 551Charge relay systemUniRule annotation
Active sitei130 – 1301Charge relay systemUniRule annotation
Active sitei154 – 1541Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMVAN406327:GI04-835-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Mevan_0822
OrganismiMethanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)
Taxonomic identifieri406327 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000001107 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431Glutamyl-tRNA(Gln) amidotransferase subunit APRO_1000015864Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi406327.Mevan_0822.

Structurei

3D structure databases

ProteinModelPortaliA6UQF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01717. Archaea.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiVAGHDKM.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6UQF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITDRVSDYL EKIEKSDINA FIDLNGERVL KEAKDLEKNE KLKNKSLYGK
60 70 80 90 100
IIAVKSNINV EGYKISCASK TLDNYTGTYD ATVIKKLRSE GALIIGMTNM
110 120 130 140 150
DEFASGSSGE TSYYGPTKNP KALDRIPGGS SSGSAAAVSA DLCDMALGSD
160 170 180 190 200
TGGSIRNPAS HCGVVGFKPT YGVVSRQGLC DLAMSFDQIG PLTKTAEDSL
210 220 230 240 250
NLTNVIKGLD LSNTTSLKTP KFEKKEIKDY KIGVVREFMD VTDEKIRNEI
260 270 280 290 300
EKGIEVFRDL GCEIVDLSYK YIDLALPTYY LINYVEFYSA TRKYDGRRFG
310 320 330 340 350
EVIEEACGEE VLRRILIGKH ISEQEFSGKY YKKALQARKE MKKEMIGLFN
360 370 380 390 400
SVDLIVGPTV PKLPHKIGEE LSPMEMYAYD VLTVLTNICG ICSGVVGCGN
410 420 430
INGIPVGLQI QGASLDDEKV LSAMIEFENN Y
Length:431
Mass (Da):47,454
Last modified:August 21, 2007 - v1
Checksum:iF09DD695E5E27238
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000742 Genomic DNA. Translation: ABR54728.1.
RefSeqiWP_011972629.1. NC_009634.1.

Genome annotation databases

EnsemblBacteriaiABR54728; ABR54728; Mevan_0822.
GeneIDi5325110.
KEGGimvn:Mevan_0822.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000742 Genomic DNA. Translation: ABR54728.1.
RefSeqiWP_011972629.1. NC_009634.1.

3D structure databases

ProteinModelPortaliA6UQF6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi406327.Mevan_0822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR54728; ABR54728; Mevan_0822.
GeneIDi5325110.
KEGGimvn:Mevan_0822.

Phylogenomic databases

eggNOGiarCOG01717. Archaea.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiVAGHDKM.

Enzyme and pathway databases

BioCyciMVAN406327:GI04-835-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_METVS
AccessioniPrimary (citable) accession number: A6UQF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: September 7, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.