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A6UPD9

- ENDA_METVS

UniProt

A6UPD9 - ENDA_METVS

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Protein
tRNA-splicing endonuclease
Gene
endA, Mevan_0454
Organism
Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141 By similarity
Active sitei123 – 1231 By similarity
Active sitei154 – 1541 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciMVAN406327:GI04-459-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease
Gene namesi
Name:endA
Ordered Locus Names:Mevan_0454
OrganismiMethanococcus vannielii (strain SB / ATCC 35089 / DSM 1224)
Taxonomic identifieri406327 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000001107: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 177177tRNA-splicing endonucleaseUniRule annotation
PRO_0000309818Add
BLAST

Interactioni

Subunit structurei

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.

Protein-protein interaction databases

STRINGi406327.Mevan_0454.

Structurei

3D structure databases

ProteinModelPortaliA6UPD9.
SMRiA6UPD9. Positions 9-176.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000107823.
KOiK01170.
OMAiNMGYLRL.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01833. EndA_short.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

A6UPD9-1 [UniParc]FASTAAdd to Basket

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MMAKPKKTIP AKLSDERIVI YDKDGISRLN EKRYGELHEN FLSLSYVEAL    50
YLVAKDWVLI RDKKNKILSF EELHEIAHVI DKKLCIRYLV YKDLRNRGYT 100
VRTGLKYGSD FRLYERSNID EVHSKYLVKV FSEEIPCEIS EITGFVRVAH 150
SVRKELVIAI VDADGSVVYY NMGYLKL 177
Length:177
Mass (Da):20,714
Last modified:August 21, 2007 - v1
Checksum:i80425F7484E7A95C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000742 Genomic DNA. Translation: ABR54361.1.
RefSeqiWP_011972264.1. NC_009634.1.
YP_001322973.1. NC_009634.1.

Genome annotation databases

EnsemblBacteriaiABR54361; ABR54361; Mevan_0454.
GeneIDi5325328.
KEGGimvn:Mevan_0454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000742 Genomic DNA. Translation: ABR54361.1 .
RefSeqi WP_011972264.1. NC_009634.1.
YP_001322973.1. NC_009634.1.

3D structure databases

ProteinModelPortali A6UPD9.
SMRi A6UPD9. Positions 9-176.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 406327.Mevan_0454.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR54361 ; ABR54361 ; Mevan_0454 .
GeneIDi 5325328.
KEGGi mvn:Mevan_0454.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000107823.
KOi K01170.
OMAi NMGYLRL.

Enzyme and pathway databases

BioCyci MVAN406327:GI04-459-MONOMER.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
HAMAPi MF_01833. EndA_short.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMi SSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SB / ATCC 35089 / DSM 1224.

Entry informationi

Entry nameiENDA_METVS
AccessioniPrimary (citable) accession number: A6UPD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: August 21, 2007
Last modified: September 3, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi