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A6UPD9

- ENDA_METVS

UniProt

A6UPD9 - ENDA_METVS

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei114 – 1141UniRule annotation
    Active sitei123 – 1231UniRule annotation
    Active sitei154 – 1541UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciMVAN406327:GI04-459-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:Mevan_0454
    OrganismiMethanococcus vannielii (strain SB / ATCC 35089 / DSM 1224)
    Taxonomic identifieri406327 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
    ProteomesiUP000001107: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 177177tRNA-splicing endonucleasePRO_0000309818Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi406327.Mevan_0454.

    Structurei

    3D structure databases

    ProteinModelPortaliA6UPD9.
    SMRiA6UPD9. Positions 9-176.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000107823.
    KOiK01170.
    OMAiNMGYLRL.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    HAMAPiMF_01833. EndA_short.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR016442. tRNA_splic_arch_short.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005285. tRNA_splic_archaea. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A6UPD9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMAKPKKTIP AKLSDERIVI YDKDGISRLN EKRYGELHEN FLSLSYVEAL    50
    YLVAKDWVLI RDKKNKILSF EELHEIAHVI DKKLCIRYLV YKDLRNRGYT 100
    VRTGLKYGSD FRLYERSNID EVHSKYLVKV FSEEIPCEIS EITGFVRVAH 150
    SVRKELVIAI VDADGSVVYY NMGYLKL 177
    Length:177
    Mass (Da):20,714
    Last modified:August 21, 2007 - v1
    Checksum:i80425F7484E7A95C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000742 Genomic DNA. Translation: ABR54361.1.
    RefSeqiWP_011972264.1. NC_009634.1.
    YP_001322973.1. NC_009634.1.

    Genome annotation databases

    EnsemblBacteriaiABR54361; ABR54361; Mevan_0454.
    GeneIDi5325328.
    KEGGimvn:Mevan_0454.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000742 Genomic DNA. Translation: ABR54361.1 .
    RefSeqi WP_011972264.1. NC_009634.1.
    YP_001322973.1. NC_009634.1.

    3D structure databases

    ProteinModelPortali A6UPD9.
    SMRi A6UPD9. Positions 9-176.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 406327.Mevan_0454.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR54361 ; ABR54361 ; Mevan_0454 .
    GeneIDi 5325328.
    KEGGi mvn:Mevan_0454.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000107823.
    KOi K01170.
    OMAi NMGYLRL.

    Enzyme and pathway databases

    BioCyci MVAN406327:GI04-459-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    HAMAPi MF_01833. EndA_short.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR016442. tRNA_splic_arch_short.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005285. tRNA_splic_archaea. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SB / ATCC 35089 / DSM 1224.

    Entry informationi

    Entry nameiENDA_METVS
    AccessioniPrimary (citable) accession number: A6UPD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3