Reviewed, UniProtKB/Swiss-Prot A6UN70 (GUAAB_METVS)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit B
    EC=6.3.5.2
Alternative name(s):
    GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	Mevan_0027

Organism

Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) [Complete proteome] [HAMAP]

Taxonomic identifier

406327 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential. HAMAP MF_00345
Sequence similarities	Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_1000048380
Regions
	Domain	33 – 186	154	GMP-binding
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6UN70-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 7F4169988830DC42	FASTA	310	34,735
	10 20 30 40 50 60 MFDAKSFIEE SVEDIKKQIN GRKTIIALSG GVDSSVAAVL TGKAIGDQLL AVYVDTGLMR 70 80 90 100 110 120 KNESNEIWNI FKEQMGLNLK IVEAKDLFLS ELAGIDDPEQ KRKIIGRIFI EVFEKVAKEQ 130 140 150 160 170 180 GEEILVQGTI APDWIESEGQ IKTHHNIALP SGMVLEVVEP LRDLYKDEVR KLATELGLPE 190 200 210 220 230 240 KIAHRQPFPG PGLAVRILGE ITEEKLEICK EANFIVSEEI EKTGLQKELW QYFAAVLDTK 250 260 270 280 290 300 ATGVKGDIRD YNWVVALRFV KSLDAMTAHT PEIPFDLIKR ISKRITSEIP NVTRVVLDVT 310 DKPPATIEFE

References

[1]

"Complete sequence of Methanococcus vannielii SB." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I. , Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000742 Genomic DNA. Translation: ABR53942.1.
RefSeq	YP_001322554.1.
3D structure databases
SMR	A6UN70. Positions 3-310.
ModBase	Search...
Protein-protein interaction databases
STRING	A6UN70.
Genome annotation databases
GeneID	5325732.
GenomeReviews	Gene locus Mevan_0027 in contig CP000742_GR.
KEGG	mvn:Mevan_0027.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04539.
HOGENOM	HBG391775.
OMA	GIKSHHN.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GUAAB_METVS

Accession

Primary (citable) accession number: A6UN70

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	February 9, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)