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Protein

Catalase-peroxidase

Gene

katG

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei94 – 941Transition state stabilizerUniRule annotation
Active sitei98 – 981Proton acceptorUniRule annotation
Metal bindingi260 – 2601Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciSMED366394:GJAL-5393-MONOMER.

Protein family/group databases

PeroxiBasei5441. SmedCP01.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:Smed_5331
Encoded oniPlasmid pSMED020 Publication
OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
Taxonomic identifieri366394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001108 Componenti: Plasmid pSMED02

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Catalase-peroxidasePRO_0000354935Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki97 ↔ 219Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-245)UniRule annotation
Cross-linki219 ↔ 245Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiA6UKC3.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6UKC3.
SMRiA6UKC3. Positions 22-728.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6UKC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMDQKTDNA GKCPVAHTAP RGRSNRDWWP DQLDVQVLHR HSDLSDPMGK
60 70 80 90 100
SFNYAEEFRK LDLDALKRDL HALMTDSQDW WPADFGHYGG LFIRMAWHSA
110 120 130 140 150
GTYRITDGRG GAGQGQQRFA PLNSWPDNAN LDKARRLLWP IKQKYGNRIS
160 170 180 190 200
WADLLILTGN VALESMGFKT FGFAGGRTDV WEPEELFWGP EGTWLGDERY
210 220 230 240 250
SGERQLSEPL AAVQMGLIYV NPEGPNGNPD PVAAAHDIRE TFARMAMNDE
260 270 280 290 300
ETVALIAGGH TFGKTHGAGD PSFIGADPEG GAIEDQGLGW KSTFGTGVGK
310 320 330 340 350
DAITGGPEVT WSQTPTRWSN YFFENLFNFE WELSKSPAGA HQWKAKNAEA
360 370 380 390 400
SVPDAYDATR KHVPTMLTTD LSLRFDPVYE KISRRFLENP DQFADAFARA
410 420 430 440 450
WFKLTHRDMG PKVRYLGPEV PAEDLIWQDV IPPVDHELVD DADVAGLKAK
460 470 480 490 500
ILASSLSVQE LVSTAWDSAS TFRGSDKRGG ANGARIRLAP QKDWEVNQPA
510 520 530 540 550
QLARVLSVLE GIQRDFNASQ AGGKKISLAD LIVLAGNAGV EKAARAAGQE
560 570 580 590 600
IIVPFTPGRM DASEAQTDAA SFAALEPRAD GFRNYVNSSR LQFMKPEEAL
610 620 630 640 650
VDRAQLLTLT APEMTVLVGG LRVLKAGQPE HGVFTSRPEA LTNDFFVNLL
660 670 680 690 700
DMGTQWSPVE GKEGVYEGRD RKTGAARWTG TRVDLIFGSH SQLRAFAEVY
710 720
AQTDAREKFV KDFVAAWTKV MNADRFDLV
Length:729
Mass (Da):80,563
Last modified:August 21, 2007 - v1
Checksum:iA1C98DF2CE362C49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000740 Genomic DNA. Translation: ABR64103.1.
RefSeqiWP_011970212.1. NC_009621.1.
YP_001314036.1. NC_009621.1.

Genome annotation databases

EnsemblBacteriaiABR64103; ABR64103; Smed_5331.
GeneIDi5319633.
KEGGismd:Smed_5331.
PATRICi23616302. VBISinMed134228_1821.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000740 Genomic DNA. Translation: ABR64103.1.
RefSeqiWP_011970212.1. NC_009621.1.
YP_001314036.1. NC_009621.1.

3D structure databases

ProteinModelPortaliA6UKC3.
SMRiA6UKC3. Positions 22-728.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

PeroxiBasei5441. SmedCP01.

Proteomic databases

PRIDEiA6UKC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR64103; ABR64103; Smed_5331.
GeneIDi5319633.
KEGGismd:Smed_5331.
PATRICi23616302. VBISinMed134228_1821.

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.
OrthoDBiPOG091H05R1.

Enzyme and pathway databases

BioCyciSMED366394:GJAL-5393-MONOMER.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG_SINMW
AccessioniPrimary (citable) accession number: A6UKC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: August 21, 2007
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.