A6UHL2 (A6UHL2_SINMW) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Smed_4337

Encoded on

Plasmid pSMED01 EMBL ABR63142.1

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 NAD SAAS SAAS003694 HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 EMBL ABR63142.1
Technical term	Complete proteome Plasmid EMBL ABR63142.1
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	47 – 54	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	49	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A6UHL2 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: D8BCBD7A85840AFD	FASTA	335	37,060
	10 20 30 40 50 60 MNIRPDQNRL AFSADPLKID EAAETDRIVA GLRAQLRSLR KRGLVLGLSG GIDSSVSVAL 70 80 90 100 110 120 AVRAVGAKNV FCLFMPENDS DPESLRLGRL IAETFGVEAV VEDIGPTLDA MGCYQRRDAF 130 140 150 160 170 180 IRELVPEYGQ GWASKIVIAN ALEGDGYNIS SLVVQDPDGQ QTKLRMPPSV YLGIVAATNM 190 200 210 220 230 240 KQRTRKQIEY FHADRLNFAV LGTPNRLEYD QGFFVKNGDG AADVKPIAHL YKSQVYALAA 250 260 270 280 290 300 HLGIPEEIRR RPPTTDTYSL EQTQEEFYFS LPYDRMDLCL FGLNSGFGAD EVGRAAGLSS 310 320 330 AQVERVWADI TAKRKATRYL HLGPQLVEPV QEIEH

References

[1]

"Complete sequence of Sinorhizobium medicae WSM419 plasmid pSMED01." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000739 Genomic DNA. Translation: ABR63142.1.
RefSeq	YP_001313075.1. NC_009620.1.
3D structure databases
ProteinModelPortal	A6UHL2.
ModBase	Search...
Protein-protein interaction databases
STRING	A6UHL2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5318095.
GenomeReviews	Gene locus Smed_4337 in contig CP000739_GR.
KEGG	smd:Smed_4337.
PATRIC	23614267. VBISinMed134228_0806.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	AHLYKSQ.
ProtClustDB	PRK00876.
Enzyme and pathway databases
BioCyc	SMED366394:SMED_4337-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A6UHL2_SINMW

Accession

Primary (citable) accession number: A6UHL2

Entry history

Integrated into UniProtKB/TrEMBL:	August 21, 2007
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)