Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A6UF34

- PNP_SINMW

UniProt

A6UF34 - PNP_SINMW

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi488 – 4881MagnesiumUniRule annotation
    Metal bindingi494 – 4941MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciSMED366394:GJAL-3505-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:Smed_3446
    OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
    Taxonomic identifieri366394 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001108: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 714714Polyribonucleotide nucleotidyltransferasePRO_0000329855Add
    BLAST

    Proteomic databases

    ProMEXiA6UF34.

    Interactioni

    Protein-protein interaction databases

    STRINGi366394.Smed_3446.

    Structurei

    3D structure databases

    ProteinModelPortaliA6UF34.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini555 – 61460KHUniRule annotationAdd
    BLAST
    Domaini624 – 69269S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218327.
    KOiK00962.
    OMAiHGNFKSN.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6UF34-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFETHKVEIE WAGRPLKLET GKIARQADGA VLATYGETVV LATVVSAKAP    50
    KPGQDFFPLT VNYQEKTYAA GKIPGGYFKR EGRPSENETL VSRLIDRPIR 100
    PLFPEGYKND TQVIITVMQH DLENNPDVVA MVAASAALTL SGVPFMGPVG 150
    GARVGYINGE YVLNPHLDEM DESTLDLVVA GTQDAVLMVE SEAKELSEEI 200
    MLGAVVFGQK GFQPVIDAVI RLAEVAAKEP REFEPDDHSA LENAMLSIAE 250
    EDLRKAYKIT EKAQRYAAVD AVKAKVKEHF LPEGVENPAH TAEEIASVFK 300
    HLQAKIVRWN ILDTKSRIDG RDLVTVRPIV AEVGLLPRTH GSALFTRGET 350
    QAIVVATLGT GEDEQYVDSL TGMYKENFML HYNFPPYSVG ETGRMGSPGR 400
    REIGHGKLAW RAIHPMLPTA EQFPYTLRVV SEITESNGSS SMATVCGTSL 450
    ALMDAGVPLA KPVAGIAMGL IKEDDRFAVL SDILGDEDHL GDMDFKVAGT 500
    EAGVTSLQMD IKIAGITEEI MKVALDQAKH GRVHILGEMA KAISESRGQL 550
    GEFAPRIEVM NIPVDKIREV IGSGGKVIRE IVEKTGAKIN IDDDGTVKIA 600
    SASGKEIEAA RKWIHSIVAE PEVGQVYEGT VVKTADFGAF VNFFGARDGL 650
    VHISQLASER VAKTTDVVKE GDKVWVKLMG FDERGKVRLS MKVVDQATGK 700
    EIAAEKKDGG EAAE 714
    Length:714
    Mass (Da):77,456
    Last modified:August 21, 2007 - v1
    Checksum:iFC29CF5FEEF898FA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000738 Genomic DNA. Translation: ABR62264.1.
    RefSeqiYP_001329099.1. NC_009636.1.

    Genome annotation databases

    EnsemblBacteriaiABR62264; ABR62264; Smed_3446.
    GeneIDi5324332.
    KEGGismd:Smed_3446.
    PATRICi23626171. VBISinMed134228_6696.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000738 Genomic DNA. Translation: ABR62264.1 .
    RefSeqi YP_001329099.1. NC_009636.1.

    3D structure databases

    ProteinModelPortali A6UF34.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 366394.Smed_3446.

    Proteomic databases

    ProMEXi A6UF34.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR62264 ; ABR62264 ; Smed_3446 .
    GeneIDi 5324332.
    KEGGi smd:Smed_3446.
    PATRICi 23626171. VBISinMed134228_6696.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218327.
    KOi K00962.
    OMAi HGNFKSN.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci SMED366394:GJAL-3505-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Sinorhizobium medicae WSM419 chromosome."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WSM419.

    Entry informationi

    Entry nameiPNP_SINMW
    AccessioniPrimary (citable) accession number: A6UF34
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3