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A6UEL4

- MASZ_SINMW

UniProt

A6UEL4 - MASZ_SINMW

Protein

Malate synthase G

Gene

glcB

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei274 – 2741Acetyl-CoAUniRule annotation
    Binding sitei311 – 3111Acetyl-CoAUniRule annotation
    Active sitei338 – 3381Proton acceptorUniRule annotation
    Binding sitei338 – 3381GlyoxylateUniRule annotation
    Metal bindingi430 – 4301MagnesiumUniRule annotation
    Binding sitei430 – 4301GlyoxylateUniRule annotation
    Metal bindingi458 – 4581MagnesiumUniRule annotation
    Binding sitei539 – 5391Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei629 – 6291Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciSMED366394:GJAL-3322-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Smed_3270
    OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
    Taxonomic identifieri366394 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001108: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 724724Malate synthase GPRO_1000056926Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei615 – 6151Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi366394.Smed_3270.

    Structurei

    3D structure databases

    ProteinModelPortaliA6UEL4.
    SMRiA6UEL4. Positions 9-720.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni123 – 1242Acetyl-CoA bindingUniRule annotation
    Regioni455 – 4584Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A6UEL4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRVEQDGLK IDAGLHRFLV EEAMPGTGVD PERFFSAFSD LIHDLGPKNR    50
    ALLVKRDELQ AKLDGWYRDH GAPVDMDAYE AFLKEIGYLL PEGSDFSVST 100
    ANVDPEIATI AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAIPDAE 150
    GAERGKGYNP KRGGRVIAWA RDFLDASAPL SSGRWIDATS LAIEGAALTV 200
    TLANGAKTAL AMPAQFAGYS GNAAAPSEIV LRKNGLHVAI VLDPTTPIGK 250
    ADAAGIADII LESAITTIMD CEDSVAAVDA EDKVLVYRNW LGLMKGDLEE 300
    QVAKGTTTFI RRLNPDRIYT ASGGDKLTLP GRSLMLVRNV GHLMTNPAIL 350
    DREGKEVPEG LMDAMVTALI ALHDIGRNGR RANSRSGSMY VVKPKMHGPE 400
    EVAFACETFT RVEAALGLPA NAMKMGIMDE ERRTTVNLKE CIRAARERVV 450
    FINTGFLDRT GDEIHTSMEA GAMIRKGDMK QAAWISAYEN WNVDVGLECG 500
    LSGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS PTAATLHATH 550
    YHRINVSEIQ NGLRSRSRAK LADILSVPVA ARPNWTEEEI QRELDNNAQG 600
    ILGYVVRWVD HGIGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHGIVS 650
    EAQIVETMKR MAAVVDAQNA GDPNYQPMAS RFDESIAFQA ALDLVLKGRE 700
    QPNGYTEPVL HRRRLELKTK QRAA 724
    Length:724
    Mass (Da):78,836
    Last modified:August 21, 2007 - v1
    Checksum:i72C72B6C6045D76B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000738 Genomic DNA. Translation: ABR62094.1.
    RefSeqiYP_001328929.1. NC_009636.1.

    Genome annotation databases

    EnsemblBacteriaiABR62094; ABR62094; Smed_3270.
    GeneIDi5324149.
    KEGGismd:Smed_3270.
    PATRICi23625787. VBISinMed134228_6511.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000738 Genomic DNA. Translation: ABR62094.1 .
    RefSeqi YP_001328929.1. NC_009636.1.

    3D structure databases

    ProteinModelPortali A6UEL4.
    SMRi A6UEL4. Positions 9-720.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 366394.Smed_3270.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR62094 ; ABR62094 ; Smed_3270 .
    GeneIDi 5324149.
    KEGGi smd:Smed_3270.
    PATRICi 23625787. VBISinMed134228_6511.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci SMED366394:GJAL-3322-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Sinorhizobium medicae WSM419 chromosome."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WSM419.

    Entry informationi

    Entry nameiMASZ_SINMW
    AccessioniPrimary (citable) accession number: A6UEL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3