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A6UEL4

- MASZ_SINMW

UniProt

A6UEL4 - MASZ_SINMW

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Protein
Malate synthase G
Gene
glcB, Smed_3270
Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei274 – 2741Acetyl-CoA By similarity
Binding sitei311 – 3111Acetyl-CoA By similarity
Active sitei338 – 3381Proton acceptor By similarity
Binding sitei338 – 3381Glyoxylate By similarity
Metal bindingi430 – 4301Magnesium By similarity
Binding sitei430 – 4301Glyoxylate By similarity
Metal bindingi458 – 4581Magnesium By similarity
Binding sitei539 – 5391Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei629 – 6291Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSMED366394:GJAL-3322-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Ordered Locus Names:Smed_3270
OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
Taxonomic identifieri366394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001108: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 724724Malate synthase GUniRule annotation
PRO_1000056926Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei615 – 6151Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi366394.Smed_3270.

Structurei

3D structure databases

ProteinModelPortaliA6UEL4.
SMRiA6UEL4. Positions 9-720.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242Acetyl-CoA binding By similarity
Regioni455 – 4584Glyoxylate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A6UEL4-1 [UniParc]FASTAAdd to Basket

« Hide

MNRVEQDGLK IDAGLHRFLV EEAMPGTGVD PERFFSAFSD LIHDLGPKNR    50
ALLVKRDELQ AKLDGWYRDH GAPVDMDAYE AFLKEIGYLL PEGSDFSVST 100
ANVDPEIATI AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAIPDAE 150
GAERGKGYNP KRGGRVIAWA RDFLDASAPL SSGRWIDATS LAIEGAALTV 200
TLANGAKTAL AMPAQFAGYS GNAAAPSEIV LRKNGLHVAI VLDPTTPIGK 250
ADAAGIADII LESAITTIMD CEDSVAAVDA EDKVLVYRNW LGLMKGDLEE 300
QVAKGTTTFI RRLNPDRIYT ASGGDKLTLP GRSLMLVRNV GHLMTNPAIL 350
DREGKEVPEG LMDAMVTALI ALHDIGRNGR RANSRSGSMY VVKPKMHGPE 400
EVAFACETFT RVEAALGLPA NAMKMGIMDE ERRTTVNLKE CIRAARERVV 450
FINTGFLDRT GDEIHTSMEA GAMIRKGDMK QAAWISAYEN WNVDVGLECG 500
LSGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS PTAATLHATH 550
YHRINVSEIQ NGLRSRSRAK LADILSVPVA ARPNWTEEEI QRELDNNAQG 600
ILGYVVRWVD HGIGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHGIVS 650
EAQIVETMKR MAAVVDAQNA GDPNYQPMAS RFDESIAFQA ALDLVLKGRE 700
QPNGYTEPVL HRRRLELKTK QRAA 724
Length:724
Mass (Da):78,836
Last modified:August 21, 2007 - v1
Checksum:i72C72B6C6045D76B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000738 Genomic DNA. Translation: ABR62094.1.
RefSeqiYP_001328929.1. NC_009636.1.

Genome annotation databases

EnsemblBacteriaiABR62094; ABR62094; Smed_3270.
GeneIDi5324149.
KEGGismd:Smed_3270.
PATRICi23625787. VBISinMed134228_6511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000738 Genomic DNA. Translation: ABR62094.1 .
RefSeqi YP_001328929.1. NC_009636.1.

3D structure databases

ProteinModelPortali A6UEL4.
SMRi A6UEL4. Positions 9-720.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 366394.Smed_3270.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR62094 ; ABR62094 ; Smed_3270 .
GeneIDi 5324149.
KEGGi smd:Smed_3270.
PATRICi 23625787. VBISinMed134228_6511.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci SMED366394:GJAL-3322-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Sinorhizobium medicae WSM419 chromosome."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WSM419.

Entry informationi

Entry nameiMASZ_SINMW
AccessioniPrimary (citable) accession number: A6UEL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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