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Protein

Malate synthase G

Gene

glcB

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway:iglyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei274 – 2741Acetyl-CoAUniRule annotation
Binding sitei311 – 3111Acetyl-CoAUniRule annotation
Active sitei338 – 3381Proton acceptorUniRule annotation
Binding sitei338 – 3381GlyoxylateUniRule annotation
Metal bindingi430 – 4301MagnesiumUniRule annotation
Binding sitei430 – 4301GlyoxylateUniRule annotation
Metal bindingi458 – 4581MagnesiumUniRule annotation
Binding sitei539 – 5391Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei629 – 6291Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSMED366394:GJAL-3322-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Smed_3270
OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
Taxonomic identifieri366394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001108 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 724724Malate synthase GPRO_1000056926Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei615 – 6151Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi366394.Smed_3270.

Structurei

3D structure databases

ProteinModelPortaliA6UEL4.
SMRiA6UEL4. Positions 9-720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242Acetyl-CoA bindingUniRule annotation
Regioni455 – 4584Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A6UEL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRVEQDGLK IDAGLHRFLV EEAMPGTGVD PERFFSAFSD LIHDLGPKNR
60 70 80 90 100
ALLVKRDELQ AKLDGWYRDH GAPVDMDAYE AFLKEIGYLL PEGSDFSVST
110 120 130 140 150
ANVDPEIATI AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAIPDAE
160 170 180 190 200
GAERGKGYNP KRGGRVIAWA RDFLDASAPL SSGRWIDATS LAIEGAALTV
210 220 230 240 250
TLANGAKTAL AMPAQFAGYS GNAAAPSEIV LRKNGLHVAI VLDPTTPIGK
260 270 280 290 300
ADAAGIADII LESAITTIMD CEDSVAAVDA EDKVLVYRNW LGLMKGDLEE
310 320 330 340 350
QVAKGTTTFI RRLNPDRIYT ASGGDKLTLP GRSLMLVRNV GHLMTNPAIL
360 370 380 390 400
DREGKEVPEG LMDAMVTALI ALHDIGRNGR RANSRSGSMY VVKPKMHGPE
410 420 430 440 450
EVAFACETFT RVEAALGLPA NAMKMGIMDE ERRTTVNLKE CIRAARERVV
460 470 480 490 500
FINTGFLDRT GDEIHTSMEA GAMIRKGDMK QAAWISAYEN WNVDVGLECG
510 520 530 540 550
LSGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS PTAATLHATH
560 570 580 590 600
YHRINVSEIQ NGLRSRSRAK LADILSVPVA ARPNWTEEEI QRELDNNAQG
610 620 630 640 650
ILGYVVRWVD HGIGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHGIVS
660 670 680 690 700
EAQIVETMKR MAAVVDAQNA GDPNYQPMAS RFDESIAFQA ALDLVLKGRE
710 720
QPNGYTEPVL HRRRLELKTK QRAA
Length:724
Mass (Da):78,836
Last modified:August 21, 2007 - v1
Checksum:i72C72B6C6045D76B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000738 Genomic DNA. Translation: ABR62094.1.
RefSeqiWP_012067475.1. NC_009636.1.
YP_001328929.1. NC_009636.1.

Genome annotation databases

EnsemblBacteriaiABR62094; ABR62094; Smed_3270.
GeneIDi5324149.
KEGGismd:Smed_3270.
PATRICi23625787. VBISinMed134228_6511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000738 Genomic DNA. Translation: ABR62094.1.
RefSeqiWP_012067475.1. NC_009636.1.
YP_001328929.1. NC_009636.1.

3D structure databases

ProteinModelPortaliA6UEL4.
SMRiA6UEL4. Positions 9-720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi366394.Smed_3270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR62094; ABR62094; Smed_3270.
GeneIDi5324149.
KEGGismd:Smed_3270.
PATRICi23625787. VBISinMed134228_6511.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciSMED366394:GJAL-3322-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Sinorhizobium medicae WSM419 chromosome."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WSM419.

Entry informationi

Entry nameiMASZ_SINMW
AccessioniPrimary (citable) accession number: A6UEL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: May 27, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.