A6UEL4 (MASZ_SINMW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Smed_3270

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium medicae

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00641
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Malate synthase G HAMAP-Rule MF_00641		PRO_1000056926
Sites
	Active site	338	1	Proton acceptor By similarity
	Active site	629	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6UEL4 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 72C72B6C6045D76B	FASTA	724	78,836
	10 20 30 40 50 60 MNRVEQDGLK IDAGLHRFLV EEAMPGTGVD PERFFSAFSD LIHDLGPKNR ALLVKRDELQ 70 80 90 100 110 120 AKLDGWYRDH GAPVDMDAYE AFLKEIGYLL PEGSDFSVST ANVDPEIATI AGPQLVVPVM 130 140 150 160 170 180 NARYALNAAN ARWGSLYDAL YGTDAIPDAE GAERGKGYNP KRGGRVIAWA RDFLDASAPL 190 200 210 220 230 240 SSGRWIDATS LAIEGAALTV TLANGAKTAL AMPAQFAGYS GNAAAPSEIV LRKNGLHVAI 250 260 270 280 290 300 VLDPTTPIGK ADAAGIADII LESAITTIMD CEDSVAAVDA EDKVLVYRNW LGLMKGDLEE 310 320 330 340 350 360 QVAKGTTTFI RRLNPDRIYT ASGGDKLTLP GRSLMLVRNV GHLMTNPAIL DREGKEVPEG 370 380 390 400 410 420 LMDAMVTALI ALHDIGRNGR RANSRSGSMY VVKPKMHGPE EVAFACETFT RVEAALGLPA 430 440 450 460 470 480 NAMKMGIMDE ERRTTVNLKE CIRAARERVV FINTGFLDRT GDEIHTSMEA GAMIRKGDMK 490 500 510 520 530 540 QAAWISAYEN WNVDVGLECG LSGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS 550 560 570 580 590 600 PTAATLHATH YHRINVSEIQ NGLRSRSRAK LADILSVPVA ARPNWTEEEI QRELDNNAQG 610 620 630 640 650 660 ILGYVVRWVD HGIGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHGIVS EAQIVETMKR 670 680 690 700 710 720 MAAVVDAQNA GDPNYQPMAS RFDESIAFQA ALDLVLKGRE QPNGYTEPVL HRRRLELKTK QRAA

References

[1]

"Complete sequence of Sinorhizobium medicae WSM419 chromosome." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000738 Genomic DNA. Translation: ABR62094.1.
RefSeq	YP_001328929.1. NC_009636.1.
3D structure databases
ProteinModelPortal	A6UEL4.
SMR	A6UEL4. Positions 9-720.
ModBase	Search...
Protein-protein interaction databases
STRING	366394.Smed_3270.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABR62094; ABR62094; Smed_3270.
GeneID	5324149.
KEGG	smd:Smed_3270.
PATRIC	23625787. VBISinMed134228_6511.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HOG000220740.
KO	K01638.
OMA	VVKPKMH.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	SMED366394:GJAL-3322-MONOMER.
UniPathway	UPA00703; UER00720.
Family and domain databases
Gene3D	2.170.170.11. 2 hits.
HAMAP	MF_00641. Malate_synth_G.
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_SINMW

Accession

Primary (citable) accession number: A6UEL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program